Abstract:
:Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
de la Cruz MJ,Hattne J,Shi D,Seidler P,Rodriguez J,Reyes FE,Sawaya MR,Cascio D,Weiss SC,Kim SK,Hinck CS,Hinck AP,Calero G,Eisenberg D,Gonen Tdoi
10.1038/nmeth.4178subject
Has Abstractpub_date
2017-02-13 00:00:00pages
399-402issue
4eissn
1548-7091issn
1548-7105pii
nmeth.4178journal_volume
14pub_type
杂志文章相关文献
NATURE METHODS文献大全abstract::Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sam...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1871
更新日期:2012-02-05 00:00:00
abstract::Researchers designed an enzyme to carry out the Diels-Alder reaction, an activity not found in nature. ...
journal_title:Nature methods
pub_type: 评论,杂志文章
doi:10.1038/nmeth0910-671
更新日期:2010-09-01 00:00:00
abstract::MicroRNA depletion by sequestration produces flexible hypomorphs that mimic mutants obtained by microRNA gene targeting. ...
journal_title:Nature methods
pub_type: 评论,杂志文章
doi:10.1038/nmeth1209-873
更新日期:2009-12-01 00:00:00
abstract::We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unk...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3337
更新日期:2015-05-01 00:00:00
abstract::We have developed a single-plasmid system for the efficient bacterial expression of mutant proteins containing unnatural amino acids at specific sites designated by amber nonsense codons. In this system, multiple copies of a gene encoding an amber suppressor tRNA derived from a Methanocaldococcus jannaschii tyrosyl-tR...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth864
更新日期:2006-04-01 00:00:00
abstract::CRISPR-based genetic screens are accelerating biological discovery, but current methods have inherent limitations. Widely used pooled screens are restricted to simple readouts including cell proliferation and sortable marker proteins. Arrayed screens allow for comprehensive molecular readouts such as transcriptome pro...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4177
更新日期:2017-03-01 00:00:00
abstract::We developed a microarray hybridization-based method, 'comparative genome sequencing' (CGS), to find mutations in bacterial genomes and used it to study metronidazole resistance in H. pylori. CGS identified mutations in several genes, most likely affecting metronidazole activation, and produced no false positives in a...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth805
更新日期:2005-12-01 00:00:00
abstract::We present a noninvasive approach to track activation of ATP-gated P2X receptors and potentially other transmitter-gated cation channels that show calcium fluxes. We genetically engineered rat P2X receptors to carry calcium sensors near the channel pore and tested this as a reporter for P2X(2) receptor opening. The me...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth1144
更新日期:2008-01-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 评论,杂志文章
doi:10.1038/s41592-018-0248-z
更新日期:2018-12-01 00:00:00
abstract::Understanding how cells maintain genome integrity when challenged with DNA double-strand breaks (DSBs) is of major importance, particularly since the discovery of multiple links of DSBs with genome instability and cancer-predisposition disorders. Ionizing radiation is the agent of choice to produce DSBs in cells; howe...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.f.206
更新日期:2008-03-01 00:00:00
abstract::We developed a general approach that allows unnatural amino acids with diverse physicochemical and biological properties to be genetically encoded in mammalian cells. A mutant Escherichia coli aminoacyl-tRNA synthetase (aaRS) is first evolved in yeast to selectively aminoacylate its tRNA with the unnatural amino acid ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth1016
更新日期:2007-03-01 00:00:00
abstract::We developed genetically encoded RNA probes for characterizing localization and dynamics of mitochondrial RNA (mtRNA) in single living cells. The probes consist of two RNA-binding domains of PUMILIO1, each connected with split fragments of a fluorescent protein capable of reconstituting upon binding to a target RNA. W...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth1030
更新日期:2007-05-01 00:00:00
abstract::Combining high-mass-accuracy mass spectrometry, isobaric tagging and software for multiplexed, large-scale protein quantification, we report deep proteomic coverage of four human embryonic stem cell and four induced pluripotent stem cell lines in biological triplicate. This 24-sample comparison resulted in a very larg...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1699
更新日期:2011-09-11 00:00:00
abstract::Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. Other eukaryotes lack the auxin response but share the SCF degradation pathway, allowing us to transplant the auxi...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1401
更新日期:2009-12-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 社论
doi:10.1038/s41592-018-0257-y
更新日期:2018-12-01 00:00:00
abstract::An outstanding challenge of epigenome-wide association studies (EWASs) performed in complex tissues is the identification of the specific cell type(s) responsible for the observed differential DNA methylation. Here we present a statistical algorithm called CellDMC ( https://github.com/sjczheng/EpiDISH ), which can ide...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0213-x
更新日期:2018-12-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-019-0311-4
更新日期:2019-02-01 00:00:00
abstract::Phenotype-based small-molecule screening is a powerful method to identify molecules that regulate cellular functions. However, such screens are generally performed in vitro under conditions that do not necessarily model complex physiological conditions or disease states. Here, we use molecular cell barcoding to enable...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3992
更新日期:2016-10-01 00:00:00
abstract::Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibrobl...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1295
更新日期:2009-02-01 00:00:00
abstract::Researchers show that to reliably establish protein interaction networks from microarray data it is necessary to measure saturation binding curves for each arrayed protein and its potential binding partners. ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth1206-966
更新日期:2006-12-01 00:00:00
abstract::Recent efforts in neuroscience research have been aimed at obtaining detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of Caenorhabditis elegans has been known for more than 25 years,...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.2637
更新日期:2013-10-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 评论,新闻
doi:10.1038/s41592-018-0192-y
更新日期:2018-11-01 00:00:00
abstract::High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4183
更新日期:2017-04-01 00:00:00
abstract::We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomple...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.2301
更新日期:2013-02-01 00:00:00
abstract::Achieving information content of satisfactory breadth and depth remains a formidable challenge for proteomics. This problem is particularly relevant to the study of primary human specimens, such as tumor biopsies, which are heterogeneous and of finite quantity. Here we present a functional proteomics strategy that uni...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth778
更新日期:2005-09-01 00:00:00
abstract::The complexity of the angiogenic cascade limits cellular approaches to studying angiogenic endothelial cells (ECs). In turn, in vivo assays do not allow the analysis of the distinct cellular behavior of ECs during angiogenesis. Here we show that ECs can be grafted as spheroids into a matrix to give rise to a complex t...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.1198
更新日期:2008-05-01 00:00:00
abstract::The biomedical application of human embryonic stem (hES) cells will increasingly depend on the availability of technologies for highly controlled genetic modification. In mouse genetics, conditional mutagenesis using site-specific recombinases has become an invaluable tool for gain- and loss-of-function studies. Here ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth884
更新日期:2006-06-01 00:00:00
abstract::Electron cryotomography is currently the only method capable of visualizing cells in three dimensions at nanometer resolutions. While modern instruments produce massive amounts of tomography data containing extremely rich structural information, data processing is very labor intensive and the results are often limited...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-019-0591-8
更新日期:2019-11-01 00:00:00
abstract::Preprocessing of functional magnetic resonance imaging (fMRI) involves numerous steps to clean and standardize the data before statistical analysis. Generally, researchers create ad hoc preprocessing workflows for each dataset, building upon a large inventory of available tools. The complexity of these workflows has s...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0235-4
更新日期:2019-01-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 评论,杂志文章
doi:10.1038/s41592-018-0283-9
更新日期:2019-01-01 00:00:00