Abstract:
:We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Andenmatten N,Egarter S,Jackson AJ,Jullien N,Herman JP,Meissner Mdoi
10.1038/nmeth.2301subject
Has Abstractpub_date
2013-02-01 00:00:00pages
125-7issue
2eissn
1548-7091issn
1548-7105pii
nmeth.2301journal_volume
10pub_type
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