Abstract:
:Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Merzlyak EM,Goedhart J,Shcherbo D,Bulina ME,Shcheglov AS,Fradkov AF,Gaintzeva A,Lukyanov KA,Lukyanov S,Gadella TW,Chudakov DMdoi
10.1038/nmeth1062subject
Has Abstractpub_date
2007-07-01 00:00:00pages
555-7issue
7eissn
1548-7091issn
1548-7105pii
nmeth1062journal_volume
4pub_type
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