Abstract：
:The self-renewal and differentiation of human pluripotent stem cells (hPSCs) have typically been studied in flat, two-dimensional (2D) environments. In this Perspective, we argue that 3D model systems may be needed in addition, as they mimic the natural 3D tissue organization more closely. We survey methods that have used 3D biomaterials for expansion of undifferentiated hPSCs, directed differentiation of hPSCs and transplantation of differentiated hPSCs in vivo.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Kraehenbuehl TP,Langer R,Ferreira LSdoi
10.1038/nmeth.1671subject
Has Abstractpub_date
2011-08-30 00:00:00pages
731-6issue
9eissn
1548-7091issn
1548-7105pii
nmeth.1671journal_volume
8pub_type
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