Abstract：
:To enhance the repertoire of molecular tools for studying malaria parasite biology, we adapted a ligand-regulatable FKBP protein destabilization domain (ddFKBP) for use in P. falciparum. We destabilized the reporter yellow fluorescent protein (YFP) and the P. falciparum protease falcipain-2 in a ligand-reversible manner by tagging with ddFKBP. The swollen food vacuole phenotype of falcipain-2 knockout parasites could be rescued in a Shld1 ligand-dependent fashion by falcipain-2-ddFKBP expression.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Armstrong CM,Goldberg DEdoi
10.1038/nmeth1132subject
Has Abstractpub_date
2007-12-01 00:00:00pages
1007-9issue
12eissn
1548-7091issn
1548-7105pii
nmeth1132journal_volume
4pub_type
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