Abstract：
:The study of protein-protein interactions by mass spectrometry is an increasingly important part of post-genomics strategies to understand protein function. A variety of mass spectrometry-based approaches allow characterization of cellular protein assemblies under near-physiological conditions and subsequent assignment of individual proteins to specific molecular machines, pathways and networks, according to an increasing level of organizational complexity. An appropriate analytical strategy can be individually tailored--from an in-depth analysis of single complexes to a large-scale characterization of entire molecular pathways or even an analysis of the molecular organization of entire expressed proteomes. Here we review different options regarding protein-complex purification strategies, mass spectrometry analysis and bioinformatic methods according to the specific question that is being addressed.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Köcher T,Superti-Furga Gdoi
10.1038/nmeth1093subject
Has Abstractpub_date
2007-10-01 00:00:00pages
807-15issue
10eissn
1548-7091issn
1548-7105pii
nmeth1093journal_volume
4pub_type
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