Abstract:
:Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Diep D,Plongthongkum N,Gore A,Fung HL,Shoemaker R,Zhang Kdoi
10.1038/nmeth.1871subject
Has Abstractpub_date
2012-02-05 00:00:00pages
270-2issue
3eissn
1548-7091issn
1548-7105pii
nmeth.1871journal_volume
9pub_type
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