Abstract：
:With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. This process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. Here we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brain-mapping studies.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Chung K,Deisseroth Kdoi
10.1038/nmeth.2481subject
Has Abstractpub_date
2013-06-01 00:00:00pages
508-13issue
6eissn
1548-7091issn
1548-7105pii
nmeth.2481journal_volume
10pub_type
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