Abstract:
:The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Riabinina O,Luginbuhl D,Marr E,Liu S,Wu MN,Luo L,Potter CJdoi
10.1038/nmeth.3250subject
Has Abstractpub_date
2015-03-01 00:00:00pages
219-22, 5 p following 222issue
3eissn
1548-7091issn
1548-7105pii
nmeth.3250journal_volume
12pub_type
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