Improved and expanded Q-system reagents for genetic manipulations.

Abstract:

:The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.

journal_name

Nat Methods

journal_title

Nature methods

authors

Riabinina O,Luginbuhl D,Marr E,Liu S,Wu MN,Luo L,Potter CJ

doi

10.1038/nmeth.3250

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

219-22, 5 p following 222

issue

3

eissn

1548-7091

issn

1548-7105

pii

nmeth.3250

journal_volume

12

pub_type

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