Rapid, optimized interactomic screening.

Abstract:

:We must reliably map the interactomes of cellular macromolecular complexes in order to fully explore and understand biological systems. However, there are no methods to accurately predict how to capture a given macromolecular complex with its physiological binding partners. Here, we present a screening method that comprehensively explores the parameters affecting the stability of interactions in affinity-captured complexes, enabling the discovery of physiological binding partners in unparalleled detail. We have implemented this screen on several macromolecular complexes from a variety of organisms, revealing novel profiles for even well-studied proteins. Our approach is robust, economical and automatable, providing inroads to the rigorous, systematic dissection of cellular interactomes.

journal_name

Nat Methods

journal_title

Nature methods

authors

Hakhverdyan Z,Domanski M,Hough LE,Oroskar AA,Oroskar AR,Keegan S,Dilworth DJ,Molloy KR,Sherman V,Aitchison JD,Fenyö D,Chait BT,Jensen TH,Rout MP,LaCava J

doi

10.1038/nmeth.3395

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

553-60

issue

6

eissn

1548-7091

issn

1548-7105

pii

nmeth.3395

journal_volume

12

pub_type

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