Abstract：
:APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is far more active in cells, enabling the use of electron microscopy to resolve the submitochondrial localization of calcium uptake regulatory protein MICU1. APEX2 also permits superior enrichment of endogenous mitochondrial and endoplasmic reticulum membrane proteins.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Lam SS,Martell JD,Kamer KJ,Deerinck TJ,Ellisman MH,Mootha VK,Ting AYdoi
10.1038/nmeth.3179subject
Has Abstractpub_date
2015-01-01 00:00:00pages
51-4issue
1eissn
1548-7091issn
1548-7105pii
nmeth.3179journal_volume
12pub_type
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