Abstract：
:To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Matsumura H,Tada M,Otsuji T,Yasuchika K,Nakatsuji N,Surani A,Tada Tdoi
10.1038/nmeth973subject
Has Abstractpub_date
2007-01-01 00:00:00pages
23-5issue
1eissn
1548-7091issn
1548-7105pii
nmeth973journal_volume
4pub_type
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