Abstract:
:We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Kantevari S,Matsuzaki M,Kanemoto Y,Kasai H,Ellis-Davies GCdoi
10.1038/nmeth.1413subject
Has Abstractpub_date
2010-02-01 00:00:00pages
123-5issue
2eissn
1548-7091issn
1548-7105pii
nmeth.1413journal_volume
7pub_type
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