Two-color, two-photon uncaging of glutamate and GABA.

Abstract:

:We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and block action potentials from rat hippocampal CA1 neurons in brain slices with 720-nm and 830-nm light, respectively. Our method should be generalizable to other chemical messenger pairs.

journal_name

Nat Methods

journal_title

Nature methods

authors

Kantevari S,Matsuzaki M,Kanemoto Y,Kasai H,Ellis-Davies GC

doi

10.1038/nmeth.1413

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

123-5

issue

2

eissn

1548-7091

issn

1548-7105

pii

nmeth.1413

journal_volume

7

pub_type

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