Abstract:
:The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Chirichella M,Lisi S,Fantini M,Goracci M,Calvello M,Brandi R,Arisi I,D'Onofrio M,Di Primio C,Cattaneo Adoi
10.1038/nmeth.4144subject
Has Abstractpub_date
2017-03-01 00:00:00pages
279-282issue
3eissn
1548-7091issn
1548-7105pii
nmeth.4144journal_volume
14pub_type
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