Abstract：
:Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm3 at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as ~15 µm and at depths up to 360 µm can be discriminated.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Skocek O,Nöbauer T,Weilguny L,Martínez Traub F,Xia CN,Molodtsov MI,Grama A,Yamagata M,Aharoni D,Cox DD,Golshani P,Vaziri Adoi
10.1038/s41592-018-0008-0subject
Has Abstractpub_date
2018-06-01 00:00:00pages
429-432issue
6eissn
1548-7091issn
1548-7105pii
10.1038/s41592-018-0008-0journal_volume
15pub_type
杂志文章相关文献
NATURE METHODS文献大全abstract：:Accurately modeling cellular response to perturbations is a central goal of computational biology. While such modeling has been based on statistical, mechanistic and machine learning models in specific settings, no generalization of predictions to phenomena absent from training data (out-of-sample) has yet been demons...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-019-0494-8
更新日期：2019-08-01 00:00:00
abstract：:We describe a general approach for refining protein structure models on the basis of cryo-electron microscopy maps with near-atomic resolution. The method integrates Monte Carlo sampling with local density-guided optimization, Rosetta all-atom refinement and real-space B-factor fitting. In tests on experimental maps o...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.3286
更新日期：2015-04-01 00:00:00
abstract：:Precisely defining the roles of specific cell types is an intriguing frontier in the study of intact biological systems and has stimulated the rapid development of genetically encoded tools for observation and control. However, targeting these tools with adequate specificity remains challenging: most cell types are be...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.2996
更新日期：2014-07-01 00:00:00
abstract：:Using a line-scanning method during functional magnetic resonance imaging (fMRI), we obtained high temporal (50-ms) and spatial (50-μm) resolution information along the cortical thickness and showed that the laminar position of fMRI onset coincides with distinct neural inputs in rat somatosensory and motor cortices. T...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.2730
更新日期：2014-01-01 00:00:00
abstract：:Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in l...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.3257
更新日期：2015-03-01 00:00:00
abstract：:Electron cryotomography is currently the only method capable of visualizing cells in three dimensions at nanometer resolutions. While modern instruments produce massive amounts of tomography data containing extremely rich structural information, data processing is very labor intensive and the results are often limited...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-019-0591-8
更新日期：2019-11-01 00:00:00
abstract：:We developed a general approach that allows unnatural amino acids with diverse physicochemical and biological properties to be genetically encoded in mammalian cells. A mutant Escherichia coli aminoacyl-tRNA synthetase (aaRS) is first evolved in yeast to selectively aminoacylate its tRNA with the unnatural amino acid ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth1016
更新日期：2007-03-01 00:00:00
abstract：:We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unk...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.3337
更新日期：2015-05-01 00:00:00
abstract：:The reconstruction of neuronal populations, a key step in understanding neural circuits, remains a challenge in the presence of densely packed neurites. Here we achieved automatic reconstruction of neuronal populations by partially mimicking human strategies to separate individual neurons. For populations not resolvab...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.3662
更新日期：2016-01-01 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature methods
pub_type： 已发布勘误
doi：10.1038/s41592-019-0588-3
更新日期：2019-10-01 00:00:00
abstract：:Protein complexes are key macromolecular machines of the cell, but their description remains incomplete. We and others previously reported an experimental strategy for global characterization of native protein assemblies based on chromatographic fractionation of biological extracts coupled to precision mass spectromet...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-019-0461-4
更新日期：2019-08-01 00:00:00
abstract：:Cells adjust to changes in environmental conditions using complex regulatory programs. These cellular programs are the result of an intricate interplay between gene expression, cellular growth and protein degradation. Technologies that enable simultaneous and time-resolved measurements of these variables are necessary...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.2879
更新日期：2014-04-01 00:00:00
abstract：:The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing a...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.4144
更新日期：2017-03-01 00:00:00
abstract：:Researchers designed an enzyme to carry out the Diels-Alder reaction, an activity not found in nature. ...
journal_title：Nature methods
pub_type： 评论,杂志文章
doi：10.1038/nmeth0910-671
更新日期：2010-09-01 00:00:00
abstract：:A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new re...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.4045
更新日期：2016-12-01 00:00:00
abstract：:Gene expression is extensively regulated at the levels of mRNA stability, localization and translation. However, decoding functional RNA-regulatory features remains a limitation to understanding post-transcriptional regulation in vivo. Here, we developed RNA-element selection assay (RESA), a method that selects RNA el...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.4121
更新日期：2017-02-01 00:00:00
abstract：:We report a technique to selectively and continuously label the proteomes of individual cell types in coculture, named cell type-specific labeling using amino acid precursors (CTAP). Through transgenic expression of exogenous amino acid biosynthesis enzymes, vertebrate cells overcome their dependence on supplemented e...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.2529
更新日期：2013-08-01 00:00:00
abstract：:Despite widespread use of CRISPR, comprehensive data on the frequency and impact of Cas9-mediated off-targets in modified rodents are limited. Here we present deep-sequencing data from 81 genome-editing projects on mouse and rat genomes at 1,423 predicted off-target sites, 32 of which were confirmed, and show that hig...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-018-0011-5
更新日期：2018-07-01 00:00:00
abstract：:Microarrays have been widely used for the analysis of gene expression, but the issue of reproducibility across platforms has yet to be fully resolved. To address this apparent problem, we compared gene expression between two microarray platforms: the short oligonucleotide Affymetrix Mouse Genome 430 2.0 GeneChip and a...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth757
更新日期：2005-05-01 00:00:00
abstract：:We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitat...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.2098
更新日期：2012-07-08 00:00:00
abstract：:Massively parallel single-cell and single-nucleus RNA sequencing has opened the way to systematic tissue atlases in health and disease, but as the scale of data generation is growing, so is the need for computational pipelines for scaled analysis. Here we developed Cumulus-a cloud-based framework for analyzing large-s...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-020-0905-x
更新日期：2020-08-01 00:00:00
abstract：:The complexity of the angiogenic cascade limits cellular approaches to studying angiogenic endothelial cells (ECs). In turn, in vivo assays do not allow the analysis of the distinct cellular behavior of ECs during angiogenesis. Here we show that ECs can be grafted as spheroids into a matrix to give rise to a complex t...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.1198
更新日期：2008-05-01 00:00:00
abstract：:Generation of complex libraries of defined nucleic acid sequences can greatly aid the functional analysis of protein and gene function. Previously, such studies relied either on individually synthesized oligonucleotides or on cellular nucleic acids as the starting material. As each method has disadvantages, we have de...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth724
更新日期：2004-12-01 00:00:00
abstract：:High spatial and temporal resolution of conditional gene expression is typically difficult to achieve in whole tissues or organisms. We synthesized two reversibly inhibited, photoactivatable ('caged') doxycycline derivatives with different membrane permeabilities for precise spatial and temporal light-controlled activ...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.1340
更新日期：2009-07-01 00:00:00
abstract：:Many protein interactions are mediated by small linear motifs interacting specifically with defined families of globular domains. Quantifying the specificity of a motif requires measuring and comparing its binding affinities to all its putative target domains. To this end, we developed the high-throughput holdup assay...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.3438
更新日期：2015-08-01 00:00:00
abstract：:The mechanical properties of the cellular microenvironment and their spatiotemporal variations are thought to play a central role in sculpting embryonic tissues, maintaining organ architecture and controlling cell behavior, including cell differentiation. However, no direct in vivo and in situ measurement of mechanica...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.4101
更新日期：2017-02-01 00:00:00
abstract：:The study of protein-protein interactions by mass spectrometry is an increasingly important part of post-genomics strategies to understand protein function. A variety of mass spectrometry-based approaches allow characterization of cellular protein assemblies under near-physiological conditions and subsequent assignmen...
journal_title：Nature methods
pub_type： 杂志文章,评审
doi：10.1038/nmeth1093
更新日期：2007-10-01 00:00:00
abstract：:The ability to image RNA identity and location with nanoscale precision in intact tissues is of great interest for defining cell types and states in normal and pathological biological settings. Here, we present a strategy for expansion microscopy of RNA. We developed a small-molecule linker that enables RNA to be cova...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.3899
更新日期：2016-08-01 00:00:00
abstract：:In mass-spectrometry-based proteomics, the identification and quantification of peptides and proteins heavily rely on sequence database searching or spectral library matching. The lack of accurate predictive models for fragment ion intensities impairs the realization of the full potential of these approaches. Here, we...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/s41592-019-0426-7
更新日期：2019-06-01 00:00:00
abstract：:We developed a caged GABA (gamma-aminobutyric acid), which, when combined with an appropriate caged glutamate, allows bimodal control of neuronal membrane potential with subcellular resolution using optically independent two-photon uncaging of each neurotransmitter. We used two-color, two-photon uncaging to fire and b...
journal_title：Nature methods
pub_type： 杂志文章
doi：10.1038/nmeth.1413
更新日期：2010-02-01 00:00:00
