Abstract：
:It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Shi Y,Pellarin R,Fridy PC,Fernandez-Martinez J,Thompson MK,Li Y,Wang QJ,Sali A,Rout MP,Chait BTdoi
10.1038/nmeth.3617subject
Has Abstractpub_date
2015-12-01 00:00:00pages
1135-8issue
12eissn
1548-7091issn
1548-7105pii
nmeth.3617journal_volume
12pub_type
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