Abstract:
:Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Gu H,Bock C,Mikkelsen TS,Jäger N,Smith ZD,Tomazou E,Gnirke A,Lander ES,Meissner Adoi
10.1038/nmeth.1414subject
Has Abstractpub_date
2010-02-01 00:00:00pages
133-6issue
2eissn
1548-7091issn
1548-7105pii
nmeth.1414journal_volume
7pub_type
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