Abstract：
:We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Xu L,Brito IL,Alm EJ,Blainey PCdoi
10.1038/nmeth.3955subject
Has Abstractpub_date
2016-09-01 00:00:00pages
759-62issue
9eissn
1548-7091issn
1548-7105pii
nmeth.3955journal_volume
13pub_type
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