Abstract:
:We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.
journal_name
Nat Methodsjournal_title
Nature methodsauthors
Chang BJ,Kittisopikul M,Dean KM,Roudot P,Welf ES,Fiolka Rdoi
10.1038/s41592-019-0327-9subject
Has Abstractpub_date
2019-03-01 00:00:00pages
235-238issue
3eissn
1548-7091issn
1548-7105pii
10.1038/s41592-019-0327-9journal_volume
16pub_type
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