Abstract:
:Expression of the high-affinity receptor for IgE (FcεRI) provides mast cells with the ability to react in a proinflammatory manner to antigens (Ags). In particular, the immediate secretion of preformed mediators from secretory lysosomes (degranulation) is typical for FcεRI-mediated mast cell activation. In addition to the FcεRI, the stem cell factor receptor, KIT, is expressed at high levels on the surface of mast cells. KIT activation controls mast cell differentiation and survival in vivo and potently stimulates the chemotaxis of these cells. Although FcεRI and KIT initiate many of the same early signaling events in mast cells, FcεRI activation results in potent degranulation and a poor chemotactic response while KIT activation triggers very little degranulation and a strong chemotactic response. Novel data published in this issue of the European Journal of Immunology [Smrž et al. Eur. J. Immunol. 2013. 43: 1873-1882] demonstrate that actin de- and repolymerization, involved in both degranulation and chemotaxis, make all the difference: Pharmacological suppression of F-actin formation converts activated KIT into a strong degranulator. The possible implications for mast cell physiology and pathophysiology are discussed in this Commentary.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Huber Mdoi
10.1002/eji.201343706subject
Has Abstractpub_date
2013-07-01 00:00:00pages
1698-701issue
7eissn
0014-2980issn
1521-4141journal_volume
43pub_type
评论,杂志文章abstract::Staphylococcus aureus is the main pathogen associated with septic arthritis. Upon infection, neutrophils are quickly recruited to the joint by different chemoattractants, especially CXCR1/2 binding chemokines. Although their excessive accumulation is associated with intense pain and permanent articular damage, neutrop...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747198
更新日期:2018-03-01 00:00:00
abstract::Biopsy specimens were obtained from the bronchial or the nasal mucosa of three patients with grass pollen-induced bronchial asthma or rhinitis 48 h after positive bronchial or nasal provocation test with grass pollen extract. T cell clones (TCC), derived from these and control specimens, were then assessed for their p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230707
更新日期:1993-07-01 00:00:00
abstract::The regulation of neutrophil half-life by members of the coagulation cascade is critical for the resolution of the inflammatory response. We have demonstrated that soluble fibrinogen (sFbg) delays human neutrophil (PMN) apoptosis through a mechanism that involves CD11b interactions, and phosphorylation of focal adhesi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323512
更新日期:2003-05-01 00:00:00
abstract::The phosphodiesterase inhibitor oxpentifylline (OXP) has a number of potentially important immunomodulatory actions which include a selective inhibition of the Th1 subset of CD4+ cells in vitro and inhibition of tumor necrosis factor (TNF)-alpha mRNA transcription. In vivo, it has a dramatic protective effect against ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251029
更新日期:1995-10-01 00:00:00
abstract::Interleukin-12 (IL-12) has been shown to be produced by monocytes by the ligation of CD40. In the present experiments, IL-12 is shown to be produced by murine spleen antigen-presenting cells (APC) by interaction with T helper 1 (Th1) clones through CD40-CD40 ligand (CD40L) interaction, but not with Th2 clones. The IL-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260318
更新日期:1996-03-01 00:00:00
abstract::The aim of this study was to investigate malignant lymphomas of donor origin induced in F1 mice undergoing a chronic graft-versus-host reaction (GVHR) after injection of parental strain spleen cells. A total of 3 X 10(8) or 4 X 10(8) C57BL/10 spleen cells were administered to 7-8-week-old H-2 incompatible (C57BL/10 X ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050610
更新日期:1976-06-01 00:00:00
abstract::Memory T cells can now be defined with various monoclonal antibodies but little is known about the functional properties of these cells as compared to virgin T cells. We have studied Ca2+ mobilization and proliferation of memory and virgin cells in both the CD4 and CD8 subsets in response to phytohemagglutin-in. Using...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200913
更新日期:1990-09-01 00:00:00
abstract::To elucidate the mechanism of autoimmune disease in neonatally thymectomized (NTX) mice, we have investigated the responsiveness of the self-reactive T cells which have not undergone clonal deletion in such animals. Consistent with a recent report (Yuuki et al., Eur. J. Immunol. 1990. 20: 1475), T cells bearing V beta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210310
更新日期:1991-03-01 00:00:00
abstract::Indirect immunofluorescence revealed that 13% of BALB/c and 33% of CBA spleen cells of B type carry specific binding sites at their surface for double-stranded poly(I).poly(C). Pretreatment of BALB/c spleen cells with anti-mouse immunoglobulin serum increased the number of B cells capable of binding poly(I).poly(C) in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081213
更新日期:1978-12-01 00:00:00
abstract::Phenotypic classification of human CD8(+) T cells using three cell surface markers, CD27, CD28 and CD45RA, was recently suggested to be useful for identification of naive, memory and effector CD8(+) T cells. However, it still remains unclear whether such classification precisely reflects functional classification of C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324478
更新日期:2004-04-01 00:00:00
abstract::Naturally occurring CD4+CD25(high) regulatory T cells (nTreg) are essential for maintaining tolerance. FOXP3 has been established as a molecular marker of nTreg; however, FOXP3 cannot be used as a reliable marker for bona fide human nTreg since effector T cells also up-regulate FOXP3 expression upon activation. Despit...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200940117
更新日期:2009-12-01 00:00:00
abstract::Regulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we character...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747041
更新日期:2017-12-01 00:00:00
abstract::Carbon monoxide (CO) treatment improves pathogenic outcome of autoimmune diseases by promoting tolerance. However, the mechanism behind this protective tolerance is not yet defined. Here, we show in a transgenic mouse model for autoimmune diabetes that ex vivo gaseous CO (gCO)-treated DCs loaded with pancreatic β-cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242684
更新日期:2013-01-01 00:00:00
abstract::Two interleukin 5 (IL5)-specific retroviral expression vectors have been constructed containing the neomycin gene as selectable marker and either the mouse IL5 cDNA region or the rat genomic IL5 gene under the control of the thymidine kinase promoter. High viral titer supernatants derived from the transfected or infec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201226
更新日期:1990-12-01 00:00:00
abstract::Human immunodeficiency virus binds to CD4 T lymphocytes by interaction between its envelope glycoprotein gp120 and the CD4 molecule. The latter is non-covalently associated with a src-related tyrosine kinase, p56lck. CD4 cross-linking increases the activity of p56lck, leading to phosphorylation of several cellular sub...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230303
更新日期:1993-03-01 00:00:00
abstract::Thymectomy was performed on the urodele amphibian Pleurodeles waltlii Michah. from the 5th to the 20th week of larval development. Thymectomized animals were allografted at 7 months of age. The results obtained are as follows: (1) Pleurodeles thymectomized before 10 weeks always tolerated allografts: (2) Pleurodeles t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050215
更新日期:1975-02-01 00:00:00
abstract::IL-7 is a major regulator of lymphocyte homeostasis; however, little is known about the mechanisms that regulate IL-7 production. To study Il7 gene regulation in vivo, we generated a novel IL-7-reporter mouse, which allows the non-invasive quantification of Il7 gene activity in live mice and, additionally, the simulta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040441
更新日期:2010-09-01 00:00:00
abstract::One of the clear paradoxes in tumor immunology is the fact that cross-presentation of cell-associated tumor antigens to CD8(+) T cells is efficient, yet CTL generation is weak, and tumors continue to grow. We examined, for the first time whether this may be due to alterations in the phenotype or function of cross-pres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940153
更新日期:2010-06-01 00:00:00
abstract::Previously, we have shown that the intravenous (i.v.) injection of allogeneic lymphocytes across an H-2 class I-mutant disparity leads to specific skin allograft tolerance caused by irradiation-sensitive donor T cells, which function as veto cells. In the present study, we show that the i.v. injection of H-2 class II-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200232
更新日期:1990-02-01 00:00:00
abstract::Two different lines of C57BL/6 mice (IgHb) carrying complete rearranged mu chain genes from BALB/c (IgMa) were analyzed for the expression and secretion of endogenous as well as transgenic immunoglobulins at the level of single cells. Quantitation of B cells expressing endogenous IgMb by cytofluorometry, limiting dilu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200507
更新日期:1990-05-01 00:00:00
abstract::Fc gamma receptor I-positive (CD64+) and Fc gamma receptor I-negative (CD64-) monocytes were prepared from highly purified (elutriation-derived) human monocytes by cytofluorograph cell sorting, and a phenotypical and functional dissociation of the isolated CD64+ and CD64- monocyte subsets is demonstrated. Surface anal...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231213
更新日期:1993-12-01 00:00:00
abstract::Antibodies to framework determinants of the VH and V lambda fragments of MOPC 315 and antisera to the VH idiotype determinants of the A 5 A antibody were used to analyze the antigen receptors of mouse T (and B) cells. This was done by using the antibodies as inhibitors in (a) an assay in which the binding of radiolabe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100207
更新日期:1980-02-01 00:00:00
abstract::NK cells display several killer inhibitory receptors (KIR) specific for different alleles of MHC class I molecules. A family of KIR are represented by type I transmembrane proteins belonging to the immunoglobulin superfamily (Ig-SF). Besides cDNA encoding for these KIR, additional cDNA have been identified which encod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199806)28:06<1980::AID-IMM
更新日期:1998-06-01 00:00:00
abstract::The variable region nucleotide sequences of seven (five IgM and two IgG) anti-histone monoclonal antibodies from a single MRL/Mp-lpr/lpr mouse have been determined. These antibodies are not clonally related and used diverse V, D and J genes. However, six of the seven antibodies have VH segments encoded by genes from t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210721
更新日期:1991-07-01 00:00:00
abstract::The mechanisms underlying the relatively slow progression of human immunodeficiency virus type 2 (HIV-2) compared with HIV-1 infection are undefined and could be a result of more effective immune responses. We used HIV-2 and HIV-1 IFN-gamma enzyme-linked immunospot assays to evaluate CD8(+) T cell responses in antiret...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526007
更新日期:2005-05-01 00:00:00
abstract::Antisera were prepared in rabbits against the idiotypic (Id) determinants of antiallergen antibodies. These antibodies were isolated from the plasma of 10 unrelated patients by immunoabsorption. Three major allergens isolated from the house dust mite, Dermatophagoides pteronyssinus (DPT), were used. The anti-Id antise...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160520
更新日期:1986-05-01 00:00:00
abstract::A murine monoclonal IgM antibody recognizes an antigen (Mo3e) found on the surface of human peripheral blood monocytes stimulated with phorbol 12-myristate 13-acetate (PMA), lipopolysaccharide and muramyl dipeptide and blocks the monocyte response to migration inhibitory factor (MIF). We utilized Western blot and immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190423
更新日期:1989-04-01 00:00:00
abstract::A method for infection of lymphocytes with Moloney(Abelson) murine leukemia virus [M(A)-MuLV] is described. Only lymphoblasts obtained after stimulation of normal spleen cells by the B cell mitogen lipopolysaccharide (LPS) were satisfactory targets for virus-specific, secondary cytotoxic T lymphocytes (CTL), whereas s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110316
更新日期:1981-03-01 00:00:00
abstract::Beta1 integrins can provide T cell co-stimulation, but little is known concerning their downstream signaling pathways. We found that Pyk2, a focal adhesion kinase-related tyrosine kinase, is regulated by beta1 integrin signaling in human T cells. Stimulation of Jurkat T cells with the alpha4beta1 integrin ligand VCAM-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3867::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::This study was performed to investigate whether gamma delta T cells could also be divided into subsets, identified by a cytokine profile, as described for alpha beta T helper (Th) cell subsets. Cytokine production was studied in 22 gamma delta T cell clones obtained from the synovial fluid and peripheral blood of one ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240923
更新日期:1994-09-01 00:00:00