Abstract:
:Mammalian sperm are differentiated germ cells that transfer genetic material from the male to the female. Owing to this essential role in the reproductive process, an understanding of the complex mechanisms that underlie sperm function has implications ranging from the development of novel contraceptives to the treatment of male infertility. While the importance of phosphorylation in sperm differentiation, maturation and fertilization has been well established, the ability to directly determine the sites of phosphorylation within sperm proteins and to quantitate the extent of phosphorylation at these sites is a recent development that has relied almost exclusively on advances in the field of proteomics. This review will summarize the work that has been carried out to date on sperm phosphoproteomics and discuss how the resulting qualitative and quantitative information has been used to provide insight into the manner in which protein phosphorylation events modulate sperm function. The authors also present the proteomics process as it is most often utilized for the elucidation of protein expression, with a particular emphasis on the way in which the process has been modified for the analysis of protein phosphorylation in sperm.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Porambo JR,Salicioni AM,Visconti PE,Platt MDdoi
10.1586/epr.12.41subject
Has Abstractpub_date
2012-10-01 00:00:00pages
533-48issue
5eissn
1478-9450issn
1744-8387journal_volume
9pub_type
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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abstract:INTRODUCTION:An accurate diagnostic classification of thyroid lesions remains an important clinical aspect that needs to be addressed in order to avoid 'diagnostic' thyroidectomies. Among the several 'omics' techniques, proteomics is playing a pivotal role in the search for diagnostic markers. In recent years, differen...
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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pub_type: 杂志文章,评审
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journal_title:Expert review of proteomics
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pub_type: 杂志文章,评审
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
pub_type: 杂志文章
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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