Abstract:
INTRODUCTION:High-content protein microarrays in principle enable the functional interrogation of the human proteome in a broad range of applications, including biomarker discovery, profiling of immune responses, identification of enzyme substrates, and quantifying protein-small molecule, protein-protein and protein-DNA/RNA interactions. As with other microarrays, the underlying proteomic platforms are under active technological development and a range of different protein microarrays are now commercially available. However, deciphering the differences between these platforms to identify the most suitable protein microarray for the specific research question is not always straightforward. Areas covered: This review provides an overview of the technological basis, applications and limitations of some of the most commonly used full-length, recombinant protein and protein fragment microarray platforms, including ProtoArray Human Protein Microarrays, HuProt Human Proteome Microarrays, Human Protein Atlas Protein Fragment Arrays, Nucleic Acid Programmable Arrays and Immunome Protein Arrays. Expert commentary: The choice of appropriate protein microarray platform depends on the specific biological application in hand, with both more focused, lower density and higher density arrays having distinct advantages. Full-length protein arrays offer advantages in biomarker discovery profiling applications, although care is required in ensuring that the protein production and array fabrication methodology is compatible with the required downstream functionality.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Duarte JG,Blackburn JMdoi
10.1080/14789450.2017.1347042subject
Has Abstractpub_date
2017-07-01 00:00:00pages
627-641issue
7eissn
1478-9450issn
1744-8387journal_volume
14pub_type
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journal_title:Expert review of proteomics
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更新日期:2016-11-01 00:00:00
abstract::As a complementary approach to 2D-PAGE, multidimensional liquid chromatography (MDLC) separation methods have been widely applied in all kinds of biological sample investigations. MDLC coupled with mass spectrometry is playing an important role in proteome research owing to its high speed, high resolution and high sen...
journal_title:Expert review of proteomics
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abstract::Protein profiling using high-throughput tandem mass spectrometry has become a powerful method for analyzing changes in global protein expression patterns in cells and tissues as a function of developmental, physiologic and disease processes. This review summarizes the utility and practical application of multidimensio...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2.1.27
更新日期:2005-01-01 00:00:00
abstract::This review addresses the challenges of neuroproteomics and recent progress in biomarkers and tests for neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis. The review will discuss how the application of quantitative 2D gel electrophoresis, combined with app...
journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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更新日期:2005-04-01 00:00:00
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更新日期:2019-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2015.1069188
更新日期:2015-01-01 00:00:00
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更新日期:2020-02-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2015.1099436
更新日期:2015-01-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.1.121
更新日期:2007-02-01 00:00:00
abstract::The Central and Eastern European Proteomic Conference (CEEPC) proudly celebrated its 10th Anniversary with an exciting scientific program inclusive of proteome, proteomics and systems biology in Budapest, Hungary. Since 2007, CEEPC has represented 'state-of the-art' proteomics in and around Central and Eastern Europe ...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2017.1339604
更新日期:2017-07-01 00:00:00
abstract::The emergence of advanced liquid chromatography mass spectrometry technologies for characterizing very complex mixtures of proteins has greatly propelled the field of proteomics, the goal of which is the simultaneous examination of all the proteins expressed by an organism. This research area represents a paradigm shi...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.1.4.433
更新日期:2004-12-01 00:00:00
abstract::The isolation of neural stem cells from fetal and adult mammalian CNS and the demonstration of functional neurogenesis in adult CNS have offered perspectives for treatment of many devastating hereditary and acquired neurological diseases. Due to this enormous potential, neural stem cells are a subject of extensive mol...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.2.175
更新日期:2008-04-01 00:00:00
abstract::Proteomics and the study of protein-protein interactions are becoming increasingly important in our effort to understand human diseases on a system-wide level. Thanks to the development and curation of protein-interaction databases, up-to-date information on these interaction networks is accessible and publicly availa...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.09.86
更新日期:2009-12-01 00:00:00
abstract::Introduction: Duchenne muscular dystrophy is a neuromuscular disorder, which is caused by abnormalities in the DMD gene that encodes the membrane cytoskeletal protein dystrophin. Besides progressive skeletal muscle wasting, dystrophinopathy also affects non-skeletal muscle tissues, including cells in the cardio-respir...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1732214
更新日期:2020-02-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1345633
更新日期:2017-07-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.09.12
更新日期:2009-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.3.425
更新日期:2008-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2019.1691919
更新日期:2019-01-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2013.858021
更新日期:2013-12-01 00:00:00
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journal_title:Expert review of proteomics
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更新日期:2020-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1374860
更新日期:2017-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.4.603
更新日期:2008-08-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2010-12-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2018.1469982
更新日期:2018-05-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2013-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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更新日期:2018-02-01 00:00:00