Abstract:
:Introduction: Metaproteomics is an established method to obtain a comprehensive taxonomic and functional view of microbial communities. After more than a decade, we are now able to describe the promise, reality, and perspectives of metaproteomics and provide useful information about the choice of method, applications, and potential improvement strategies.Areas covered: In this article, we will discuss current challenges of species and proteome coverage, and also highlight functional aspects of metaproteomics analysis of microbial communities with different levels of complexity. To do this, we re-analyzed data from microbial communities with low to high complexity (8, 72, 200 and >300 species). High species diversity leads to a reduced number of protein group identifications in a complex community, and thus the number of species resolved is underestimated. Ultimately, low abundance species remain undiscovered in complex communities. However, we observed that the main functional categories were better represented within complex microbiomes when compared to species coverage.Expert opinion: Our findings showed that even with low species coverage, metaproteomics has the potential to reveal habitat-specific functional features. Finally, we exploit this information to highlight future research avenues that are urgently needed to enhance our understanding of taxonomic composition and functions of complex microbiomes.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Lohmann P,Schäpe SS,Haange SB,Oliphant K,Allen-Vercoe E,Jehmlich N,Von Bergen Mdoi
10.1080/14789450.2020.1738931subject
Has Abstractpub_date
2020-02-01 00:00:00pages
163-173issue
2eissn
1478-9450issn
1744-8387journal_volume
17pub_type
杂志文章abstract::Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. However, very little is known about the regulation of SC maintenance pathways in cancer and ho...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.12.24
更新日期:2012-06-01 00:00:00
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journal_title:Expert review of proteomics
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doi:10.1586/14789450.2015.1094381
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.3.363
更新日期:2007-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.09.12
更新日期:2009-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2011-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1304215
更新日期:2017-04-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1392244
更新日期:2017-12-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2019.1645603
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1345633
更新日期:2017-07-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1803743
更新日期:2020-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
doi:10.1586/epr.12.55
更新日期:2012-12-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1732214
更新日期:2020-02-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2014.926221
更新日期:2014-08-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.3.4.409
更新日期:2006-08-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2014.971116
更新日期:2014-12-01 00:00:00
abstract:INTRODUCTION:Heart diseases are a leading cause of morbidity and mortality for both men and women worldwide, and impose significant economic burdens on the healthcare systems. Despite substantial effort over the last several decades, the molecular mechanisms underlying diseases of the heart remain poorly understood. A...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2016.1209414
更新日期:2016-08-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2019.1654860
更新日期:2019-09-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1307106
更新日期:2017-04-01 00:00:00
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2013.858021
更新日期:2013-12-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2004-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.10.10
更新日期:2010-06-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.10.49
更新日期:2010-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1708199
更新日期:2020-01-01 00:00:00
abstract:INTRODUCTION:Skeletal muscle is the major site of insulin-stimulated glucose uptake and imparts the beneficial effects of exercise, and hence is an important site of insulin resistance in obesity and type 2 diabetes (T2D). Despite extensive molecular biology-oriented research the molecular mechanisms underlying insulin...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2018.1528147
更新日期:2018-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2013.837663
更新日期:2013-10-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
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更新日期:2018-08-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.5.705
更新日期:2008-10-01 00:00:00