Update on: proteome analysis in thyroid pathology - part II: overview of technical and clinical enhancement of proteomic investigation of the thyroid lesions.


INTRODUCTION:An accurate diagnostic classification of thyroid lesions remains an important clinical aspect that needs to be addressed in order to avoid 'diagnostic' thyroidectomies. Among the several 'omics' techniques, proteomics is playing a pivotal role in the search for diagnostic markers. In recent years, different approaches have been used, taking advantage of the technical improvements related to mass spectrometry that have occurred. Areas covered: The review provides an update of the recent findings in diagnostic classification, in genetic definition and in the investigation of thyroid lesions based on different proteomics approaches and on different type of specimens: cytological, surgical and biofluid samples. A brief section will discuss how these findings can be integrated with those obtained by metabolomics investigations. Expert commentary: Among the several proteomics approaches able to deepen our knowledge of the molecular alterations of the different thyroid lesions, MALDI-MSI is strongly emerging above all. In fact, MS-imaging has also been demonstrated to be capable of distinguishing thyroid lesions, based on their different molecular signatures, using cytological specimens. The possibility to use the material obtained by the fine needle aspiration makes MALDI-MSI a highly promising technology that could be implemented into the clinical and pathological units.


Expert Rev Proteomics


Piga I,Casano S,Smith A,Tettamanti S,Leni D,Capitoli G,Pincelli AI,Scardilli M,Galimberti S,Magni F,Pagni F




Has Abstract


2018-11-01 00:00:00












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    abstract:INTRODUCTION:Frailty is consequent to age-dependent deregulation of several biological pathways and systems, encompassing namely sarcopenia, age-associated hormonal derangements, inflammation, and nutritional or metabolic deficiencies. Although the prevalence of frailty is usually between 10% and 20% in the general eld...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Danese E,Montagnana M,Lippi G

    更新日期:2018-08-01 00:00:00

  • Investigation of therapy resistance mechanisms in myeloid leukemia by protein profiling of bone marrow extracellular fluid.

    abstract::In spite of the effective chronic myeloid leukemia (CML) therapy, a small percentage will fail on therapy and develop acute myeloid leukemia-like blast crisis. Understanding the underlying biology of therapy resistance is probably needed to develop better blood cancer therapy, and CML may be an ideal disease model for...

    journal_title:Expert review of proteomics

    pub_type: 评论,杂志文章


    authors: Gjertsen BT,Wiig H

    更新日期:2012-12-01 00:00:00

  • Skin proteomics - analysis of the extracellular matrix in health and disease.

    abstract:INTRODUCTION:The skin protects the human body from external insults and regulates water and temperature homeostasis. A highly developed extracellular matrix (ECM) supports the skin and instructs its cell functions. Reduced functionality of the ECM is often associated with skin diseases that cause physical impairment an...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Dengjel J,Bruckner-Tuderman L,Nyström A

    更新日期:2020-05-01 00:00:00

  • Complementary methods to assist subcellular fractionation in organellar proteomics.

    abstract::Organellar proteomics aims to describe the full complement of proteins of subcellular structures and organelles. When compared with whole-cell or whole-tissue proteomes, the more focused results from subcellular proteomic studies have yielded relatively simpler datasets from which biologically relevant information can...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Gauthier DJ,Lazure C

    更新日期:2008-08-01 00:00:00

  • Effects of preanalytical variables on peptide and protein measurements in human serum and plasma: implications for clinical proteomics.

    abstract::There is a wealth of knowledge in the field of in vitro diagnostics with regard to preanalytical variables and their impact on the determination of peptide and protein analytes in human serum and plasma. This information is applicable to clinical proteomics investigations, which utilize the same sample types. Studies ...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Rai AJ,Vitzthum F

    更新日期:2006-08-01 00:00:00

  • Targeted proteomics of solid cancers: from quantification of known biomarkers towards reading the digital proteome maps.

    abstract::The concept of personalized medicine includes novel protein biomarkers that are expected to improve the early detection, diagnosis and therapy monitoring of malignant diseases. Tissues, biofluids, cell lines and xenograft models are the common sources of biomarker candidates that require verification of clinical value...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Pernikářová V,Bouchal P

    更新日期:2015-01-01 00:00:00

  • Detecting peptidic drugs, drug candidates and analogs in sports doping: current status and future directions.

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Thevis M,Thomas A,Schänzer W

    更新日期:2014-12-01 00:00:00

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: D'Alessandro A,Zolla L

    更新日期:2011-06-01 00:00:00

  • Human saliva proteome analysis and disease biomarker discovery.

    abstract::Human saliva is an attractive body fluid for disease diagnosis and prognosis because saliva testing is simple, safe, low-cost and noninvasive. Comprehensive analysis and identification of the proteomic content in human whole and ductal saliva will not only contribute to the understanding of oral health and disease pat...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Hu S,Loo JA,Wong DT

    更新日期:2007-08-01 00:00:00

  • Sperm phosphoproteomics: historical perspectives and current methodologies.

    abstract::Mammalian sperm are differentiated germ cells that transfer genetic material from the male to the female. Owing to this essential role in the reproductive process, an understanding of the complex mechanisms that underlie sperm function has implications ranging from the development of novel contraceptives to the treatm...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Porambo JR,Salicioni AM,Visconti PE,Platt MD

    更新日期:2012-10-01 00:00:00

  • Transcriptomic profiling in Cutaneous Leishmaniasis patients.

    abstract:INTRODUCTION:Cutaneous leishmaniasis (CL), caused by different Leishmania parasite species, is associated with parasite-induced immune-mediated skin inflammation and ulceration. Whereas many CL studies focus on gene expression signatures in mouse models, the transcriptional response driving human patients in the field ...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Masoudzadeh N,Mizbani A,Rafati S

    更新日期:2020-01-01 00:00:00

  • Mass spectrometric approaches for characterizing bacterial proteomes.

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: VerBerkmoes NC,Connelly HM,Pan C,Hettich RL

    更新日期:2004-12-01 00:00:00

  • Shining a light on defective autophagy by proteomics approaches: implications for neurodegenerative illnesses.

    abstract::Introduction: Autophagy is one of the most conserved clearance systems through which eukaryotes manage to handle dysfunctional and excess organelles and macromolecules. This catabolic process has not only a role in the maintenance of basal turnover of cellular components, but it is also essential in cells adaptation t...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Di Domenico F,Zuliani I,Tramutola A

    更新日期:2019-01-01 00:00:00

  • Proteomics technologies in endometriosis.

    abstract::Endometriosis is a common disorder that is associated with infertility and pelvic pain. Diagnosis is based on the visualization of endometriotic lesions during surgery as no reliable serum marker is currently available. The etiology of endometriosis is largely unknown. Over the last 20 years, several proteomics techno...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Ferrero S,Gillott DJ,Remorgida V,Ragni N,Venturini PL,Grudzinskas JG

    更新日期:2008-10-01 00:00:00

  • Urinary biomarkers of physical activity: candidates and clinical utility.

    abstract::Chronic physical inactivity is a major risk factor for a number of important lifestyle diseases, while inappropriate exposure to high physical demands is a risk factor for musculoskeletal injury and fatigue. Proteomic and metabolomic investigations of the physical activity continuum - extreme sedentariness to extremes...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Sampson DL,Broadbent JA,Parker AW,Upton Z,Parker TJ

    更新日期:2014-02-01 00:00:00

  • CSF analysis for protein biomarker identification in patients with leptomeningeal metastases from CNS lymphoma.

    abstract:INTRODUCTION:Leptomeningeal metastases (LM) from lymphoma remain a difficult complication for oncologist due to the high incidence in morbidity and mortality. Early diagnostic and initiation of treatment are essential to prevent neurological deterioration. Areas covered: In this review, several proteomic approaches are...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Galicia N,Dégano R,Díez P,González-González M,Góngora R,Ibarrola N,Fuentes M

    更新日期:2017-04-01 00:00:00

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Gallien S,Domon B

    更新日期:2015-01-01 00:00:00

  • Helicobacter pylori immunoproteomics in gastric cancer and gastritis of the carcinoma phenotype.

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Lahner E,Bernardini G,Santucci A,Annibale B

    更新日期:2010-04-01 00:00:00

  • Clinical proteomics in obstetrics and neonatology.

    abstract::Clinical proteomics has been applied to the identification of biomarkers of obstetric and neonatal disease. We will discuss a number of encouraging studies that have led to potentially valid biomarkers in the context of Down's syndrome, preterm birth, amniotic infections, preeclampsia, intrauterine growth restriction ...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Klein J,Buffin-Meyer B,Mullen W,Carty DM,Delles C,Vlahou A,Mischak H,Decramer S,Bascands JL,Schanstra JP

    更新日期:2014-02-01 00:00:00

  • Potential blood biomarkers for stroke.

    abstract::Stroke is one of the most common causes of death worldwide and a major cause of acquired disability in adults. Despite advances in research during the last decade, prevention and treatment strategies still suffer from significant limitations, and therefore new theoretical and technical approaches are required. Technol...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Laborde CM,Mourino-Alvarez L,Akerstrom F,Padial LR,Vivanco F,Gil-Dones F,Barderas MG

    更新日期:2012-08-01 00:00:00

  • Interactomics and tick vaccine development: new directions for the control of tick-borne diseases.

    abstract:INTRODUCTION:Ticks are obligate hematophagous arthropod ectoparasites that transmit pathogens responsible for a growing number of tick-borne diseases (TBDs) throughout the world. Vaccines have been shown to be the most efficient, cost-effective, and environmentally friendly approach for the control of ticks and the pre...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Artigas-Jerónimo S,De La Fuente J,Villar M

    更新日期:2018-08-01 00:00:00

  • A decade of proteomics accomplished! Central and Eastern European Proteomic Conference (CEEPC) celebrates its 10th Anniversary in Budapest, Hungary.

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Gadher SJ,Drahos L,Vékey K,Kovarova H

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Matsumoto H,Matsumoto I

    更新日期:2008-04-01 00:00:00

  • Development of multidimensional liquid chromatography and application in proteomic analysis.

    abstract::As a complementary approach to 2D-PAGE, multidimensional liquid chromatography (MDLC) separation methods have been widely applied in all kinds of biological sample investigations. MDLC coupled with mass spectrometry is playing an important role in proteome research owing to its high speed, high resolution and high sen...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Gao M,Qi D,Zhang P,Deng C,Zhang X

    更新日期:2010-10-01 00:00:00

  • The human urinary exosome as a potential metabolic effector cargo.

    abstract::Exosomes are nanovesicles, derived from the endocytic pathway, released by most cell types and found in many body fluids, including urine. A variety of exosomal functions have been reported, including transfer of RNA, cell communication, control of apoptosis and protein lifespan. Exosomes from mesenchymal stem cells c...

    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Bruschi M,Ravera S,Santucci L,Candiano G,Bartolucci M,Calzia D,Lavarello C,Inglese E,Petretto A,Ghiggeri G,Panfoli I

    更新日期:2015-08-01 00:00:00

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章


    authors: Javitt A,Merbl Y

    更新日期:2019-07-19 00:00:00

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Skalnikova H,Vodicka P,Gadher SJ,Kovarova H

    更新日期:2008-04-01 00:00:00

  • Proteomics in studying cancer stem cell biology.

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Kranenburg O,Emmink BL,Knol J,van Houdt WJ,Rinkes IH,Jimenez CR

    更新日期:2012-06-01 00:00:00

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Boggio KJ,Obasuyi E,Sugino K,Nelson SB,Agar NY,Agar JN

    更新日期:2011-10-01 00:00:00

  • The role of mass spectrometry in the characterization of biologic protein products.

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    journal_title:Expert review of proteomics

    pub_type: 杂志文章,评审


    authors: Rathore D,Faustino A,Schiel J,Pang E,Boyne M,Rogstad S

    更新日期:2018-05-01 00:00:00