Abstract:
:Introduction: The last decade has yielded significant developments in the field of proteomics, especially in mass spectrometry (MS) and data analysis tools. In particular, a shift from gel-based to MS-based proteomics has been observed, thereby providing a platform with which to construct proteome atlases for all life forms. Nevertheless, the analysis of plant proteomes, especially those of samples that contain high-abundance proteins (HAPs), such as soybean seeds, remains challenging. Areas covered: Here, we review recent progress in soybean seed proteomics and highlight advances in HAPs depletion methods and peptide pre-fractionation, identification, and quantification methods. We also suggest a pipeline for future proteomic analysis, in order to increase the dynamic coverage of the soybean seed proteome. Expert opinion: Because HAPs limit the dynamic resolution of the soybean seed proteome, the depletion of HAPs is a prerequisite of high-throughput proteome analysis, and owing to the use of two-dimensional gel electrophoresis-based proteomic approaches, few soybean seed proteins have been identified or characterized. Recent advances in proteomic technologies, which have significantly increased the proteome coverage of other plants, could be used to overcome the current complexity and limitation of soybean seed proteomics.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Min CW,Gupta R,Agrawal GK,Rakwal R,Kim STdoi
10.1080/14789450.2019.1654860subject
Has Abstractpub_date
2019-09-01 00:00:00pages
795-804issue
9eissn
1478-9450issn
1744-8387journal_volume
16pub_type
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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journal_title:Expert review of proteomics
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