Abstract:
:These are really exciting times in the field of Parkinson's disease research. Although the etiology of sporadic disease still remains a mystery, many of the proteins associated with hereditary disease (5-10% of all disease) have now been identified. Only time will tell whether proteins associated with hereditary disease are involved in the development of sporadic disease. The most valuable proteomic studies performed to date are, and continue to be, those aimed at identifying endogenous binding partners, substrates, post-translational modifications and cellular pathways affected by these proteins. Similar to global proteomic approaches, even these approaches have surprisingly often been characterized by the production of very long lists of proteins. Consequently, the parallel development of more refined protein-protein interactions maps has aided the chance of identifying those protein complexes and/or cellular pathways, which, when disrupted, lead to the development of disease. The knowledge gained from these studies is essential, as targeting the activities of these proteins, or the pathways they operate in, currently offers the best opportunity to develop new therapeutic strategies to treat the disease. They may include agents to modulators of kinase activities (e.g., PINK1 and LRRK2), modulators of the activity of the ubiquitin-protein ligase, Parkin, proteostasis agents to block alpha-synuclein filament assembly and toxicity, or promote the refolding of mutant proteins, modulators of alpha-synuclein transfer between cells, reagents to regulate cargo dynamics along axonal microtubule networks, stimulators of autophagy and/or modulators of cellular stress pathways. The second major challenge will be to identify biomarkers to enable population screening to identify those with asymptomatic early-stage disease. Whether the analysis of blood or urine samples will yield such a marker, remains to be determined. Success or failure will be highly dependent on adopting strict standard operating procedures for the collection, processing and storage of samples, combined with the need for the identification of the most robust methods of prefractionation of samples to remove the most abundant proteins prior to proteomic screening.
journal_name
Expert Rev Proteomicsjournal_title
Expert review of proteomicsauthors
Robinson PAdoi
10.1586/epr.10.40subject
Has Abstractpub_date
2010-08-01 00:00:00pages
565-78issue
4eissn
1478-9450issn
1744-8387journal_volume
7pub_type
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journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
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abstract::Protein profiling using high-throughput tandem mass spectrometry has become a powerful method for analyzing changes in global protein expression patterns in cells and tissues as a function of developmental, physiologic and disease processes. This review summarizes the utility and practical application of multidimensio...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2.1.27
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abstract::Proteomics is performed in microgravity research in order to determine protein alterations occurring qualitatively and quantitatively, when single cells or whole organisms are exposed to real or simulated microgravity. To this purpose, antibody-dependent (Western blotting, flow cytometry, Luminex(®) technology) and an...
journal_title:Expert review of proteomics
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doi:10.1586/14789450.2014.926221
更新日期:2014-08-01 00:00:00
abstract::It is well known that chronic, excessive consumption of alcohol can cause brain damage/structural changes in the regions important for neurocognitive function. Some of the damages are permanent, while others are reversible. Molecular mechanisms underlying alcohol-induced and/or -related brain damage are largely unknow...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.2.321
更新日期:2008-04-01 00:00:00
abstract::Tissue microarrays are a high-throughput method for the investigation of biomarkers in multiple tissue specimens at once. This technique allows for the analysis of up to 500 tissue samples in a single experiment using immunohistochemistry and in situ hybridization. Recently, cell lines and xenografts have been reduced...
journal_title:Expert review of proteomics
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doi:10.1586/14789450.2.3.325
更新日期:2005-06-01 00:00:00
abstract::Ovarian cancer is the fifth leading cause of cancer deaths among North American women. Regrettably, there is currently no reliable circulating biomarker that can detect ovarian cancer in its early stages. The CA125 biomarker is very useful for treatment response monitoring, but its sensitivity is very low for early de...
journal_title:Expert review of proteomics
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doi:10.1586/14789450.4.1.121
更新日期:2007-02-01 00:00:00
abstract::As a complementary approach to 2D-PAGE, multidimensional liquid chromatography (MDLC) separation methods have been widely applied in all kinds of biological sample investigations. MDLC coupled with mass spectrometry is playing an important role in proteome research owing to its high speed, high resolution and high sen...
journal_title:Expert review of proteomics
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doi:10.1586/epr.10.49
更新日期:2010-10-01 00:00:00
abstract::Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that are implicated in a variety of diseases. Upon stress, they stabilize unfolding proteins and prevent them from aggregating. However, under physiological conditions without severe stress, some sHsps interact with other proteins. In a perspective ...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2015.1039993
更新日期:2015-06-01 00:00:00
abstract::Owing to its extensive use in the human diet, wheat is among the most common causes of food-related allergies and intolerances. Allergies to wheat are provoked by ingestion, inhalation or contact with either the soluble or the insoluble gluten proteins in wheat. Gluten proteins, and particularly the gliadin fraction, ...
journal_title:Expert review of proteomics
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doi:10.1586/epr.10.98
更新日期:2011-02-01 00:00:00
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journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.12.33
更新日期:2012-08-01 00:00:00
abstract::Endometriosis is a common disorder that is associated with infertility and pelvic pain. Diagnosis is based on the visualization of endometriotic lesions during surgery as no reliable serum marker is currently available. The etiology of endometriosis is largely unknown. Over the last 20 years, several proteomics techno...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.5.5.705
更新日期:2008-10-01 00:00:00
abstract::With the growing availability of mature systems and strategies in biotechnology and the continuously expanding knowledge of cellular processes and involved biomolecules, human sports drug testing has become a considerably complex field in the arena of analytical chemistry. Proving the exogenous origin of peptidic drug...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2014.965159
更新日期:2014-12-01 00:00:00
abstract:INTRODUCTION:Heart diseases are a leading cause of morbidity and mortality for both men and women worldwide, and impose significant economic burdens on the healthcare systems. Despite substantial effort over the last several decades, the molecular mechanisms underlying diseases of the heart remain poorly understood. A...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2016.1209414
更新日期:2016-08-01 00:00:00
abstract::Numerous efforts have been made to understand fundamental biology of diseases based on gene expression. However, the relationship between gene expression and onset of disease often remains obscure. The great advances in protein microarrays allow us to investigate this unclear question through protein profiles, which a...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.12.67
更新日期:2013-02-01 00:00:00
abstract::Protein phosphorylation events are key regulators of cellular signaling processes. In the era of functional genomics, rational drug design programs demand large-scale high-throughput analysis of signal transduction cascades. Significant improvements in the area of mass spectrometry-based proteomics have provided excit...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.1.3.343
更新日期:2004-10-01 00:00:00
abstract::Globally, colorectal cancer (CRC) is the third most common malignant neoplasm. However, highly sensitive, specific, noninvasive tests that allow CRC diagnosis at an early stage are still needed. As circulatory blood reflects the physiological status of an individual and/or the disease status for several disorders, eff...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2014.905743
更新日期:2014-08-01 00:00:00
abstract::Evaluation of: Di Girolamo F, Boschetti E, Chung MC, Guadagni F, Righetti PG. 'Proteomineering' or not? The debate on biomarker discovery in sera continues. J. Proteomics 74(5), 589-594 (2011). The combinatorial peptide ligand library in association with mass spectrometry can greatly enhance the dynamic range of the a...
journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
doi:10.1586/epr.11.43
更新日期:2011-08-01 00:00:00
abstract::Human saliva is an attractive body fluid for disease diagnosis and prognosis because saliva testing is simple, safe, low-cost and noninvasive. Comprehensive analysis and identification of the proteomic content in human whole and ductal saliva will not only contribute to the understanding of oral health and disease pat...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.4.4.531
更新日期:2007-08-01 00:00:00
abstract::This review addresses the challenges of neuroproteomics and recent progress in biomarkers and tests for neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis. The review will discuss how the application of quantitative 2D gel electrophoresis, combined with app...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.3.1.45
更新日期:2006-02-01 00:00:00
abstract::Although genomics techniques such as DNA microarrays have been widely used in virology, much more limited use has been made of proteomics. Although difficult, proteomics can greatly contribute to an understanding of virus-cell interactions, including the ternary structure of viral receptors at the cell surface, post-t...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2.4.577
更新日期:2005-08-01 00:00:00
abstract::Huntington's disease (HD) is caused by expansion of a polyglutamine repeat in the N-terminal region of huntingtin (htt), a large protein that has been found to interact with a variety of proteins. It remains to be determined how the interactions of htt with other proteins are involved in the pathogenesis of HD. A rece...
journal_title:Expert review of proteomics
pub_type: 评论,杂志文章
doi:10.1586/epr.11.72
更新日期:2012-01-01 00:00:00
abstract:INTRODUCTION:Cutaneous leishmaniasis (CL), caused by different Leishmania parasite species, is associated with parasite-induced immune-mediated skin inflammation and ulceration. Whereas many CL studies focus on gene expression signatures in mouse models, the transcriptional response driving human patients in the field ...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1080/14789450.2020.1812390
更新日期:2020-01-01 00:00:00
abstract:INTRODUCTION:An accurate diagnostic classification of thyroid lesions remains an important clinical aspect that needs to be addressed in order to avoid 'diagnostic' thyroidectomies. Among the several 'omics' techniques, proteomics is playing a pivotal role in the search for diagnostic markers. In recent years, differen...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2018.1532793
更新日期:2018-11-01 00:00:00
abstract::In recent years, MALDI imaging mass spectrometry (MALDI-IMS) has developed as a promising tool to investigate the spatial distribution of biomolecules in intact tissue specimens. Ion densities of various molecules can be displayed as heat maps while preserving anatomical structures. In this short review, an overview o...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/14789450.2013.814939
更新日期:2013-08-01 00:00:00
abstract::A panel of biomarkers for the early detection of bladder cancer has not yet been identified. Many different molecules, including DNA, RNA or proteins have been reported but none have provided adequate sensitivity for a single-tier screening test or a test to replace cystoscopy. Therefore, multimarker panels are discus...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.10.82
更新日期:2010-12-01 00:00:00
abstract:INTRODUCTION:Chromatin remodeling complexes play important roles in the control of genome regulation in both normal and diseased states, and are therefore critical components for the regulation of epigenetic states in cells. Given the role epigenetics plays in cancer, for example, chromatin remodeling complexes are rou...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2017.1374860
更新日期:2017-10-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in females of reproductive age, and its prevalence ranges between 6 and 8%. Associated problems include infertility, menstrual disorders, hirsutism and obesity. In addition, individuals with PCOS may be at increased risk of diabetes, endometrial ca...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1586/epr.09.68
更新日期:2009-10-01 00:00:00
abstract:OBJECTIVE:Hepcidin-25 production is stimulated by systemic inflammation, and it interferes with iron utilization, leading to anemia. This study aimed to investigate the relationships between the plasma levels of hepcidin, interleukin-6 (IL-6), erythropoietin (EPO) and erythroferrone (ERFE) in patients with benign breas...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/14789450.2015.1099436
更新日期:2015-01-01 00:00:00
abstract::Introduction: Autophagy is one of the most conserved clearance systems through which eukaryotes manage to handle dysfunctional and excess organelles and macromolecules. This catabolic process has not only a role in the maintenance of basal turnover of cellular components, but it is also essential in cells adaptation t...
journal_title:Expert review of proteomics
pub_type: 杂志文章,评审
doi:10.1080/14789450.2019.1691919
更新日期:2019-01-01 00:00:00
abstract::Osteoporosis is prevalent among the elderly and is a major cause of bone fracture in this population. Bone integrity is maintained by the dynamic processes of bone resorption and bone formation (bone remodeling). Osteoporosis results when there is an imbalance of the two counteracting processes. Bone mineral density, ...
journal_title:Expert review of proteomics
pub_type: 杂志文章
doi:10.1586/epr.09.90
更新日期:2010-02-01 00:00:00