Abstract：
:Recently, we identified a novel chromosomal rearrangement in Hodgkin lymphoma (HL), t(4;8)(q27;q24), which targets homeobox gene ZHX2 at the recurrent breakpoint 8q24. This aberration deletes the far upstream region of ZHX2 and results in silenced transcription pinpointing loss of activatory elements. Here, we have looked for potential binding sites within this deleted region to analyze the transcriptional deregulation of this tumor suppressor gene in B-cell malignancies. SiRNA-mediated knockdown and reporter gene analyses identified two transcription factors, homeodomain protein MSX1 and bZIP protein XBP1, directly regulating ZHX2 expression. Furthermore, MSX1-cofactor histone H1C mediated repression of ZHX2 and showed enhanced expression levels in cell line L-1236. As demonstrated by fluorescence in situ hybridization and genomic array analysis, the gene loci of MSX1 at 4p16 and H1C at 6p22 were rearranged in several HL cell lines, correlating with their altered expression activity. The expression of XBP1 was reduced in 6/7 HL cell lines as compared to primary hematopoietic cells. Taken together, our results demonstrate multiple mechanisms decreasing expression of tumor suppressor gene ZHX2 in HL cell lines: loss of enhancing binding sites, reduced expression of activators MSX1 and XBP1, and overexpression of MSX1-corepressor H1C. Moreover, chromosomal deregulations of genes involved in this regulative network highlight their role in development and malignancy of B-cells.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Nagel S,Schneider B,Meyer C,Kaufmann M,Drexler HG,Macleod RAdoi
10.1016/j.leukres.2011.10.019subject
Has Abstractpub_date
2012-05-01 00:00:00pages
646-55issue
5eissn
0145-2126issn
1873-5835pii
S0145-2126(11)00516-9journal_volume
36pub_type
杂志文章abstract：:Feulgen-DNA cytophotometry and 3H-thymidine autoradiography were used to evaluate therapeutic cytapheresis effects on cell cycle distributions of peripheral blood leukemic cells in 15 acute leukemia, 5 CML and 8 CLL patients and of bone marrow erythroblasts in 18 PV patients. Both individual cytapheresis procedures an...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90186-x
更新日期：1987-01-01 00:00:00
abstract：:A permanent lymphoblastoid cell line was established from the peripheral blood of a child with acute lymphoblastic leukemia. The cell line, designated SDK, grows in a stationary suspension culture, forming aggregates, in RPMI medium supplemented with 10% FCS, with a doubling time of 50-60 h. Immunologic markers and cy...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(85)90025-6
更新日期：1985-01-01 00:00:00
abstract：:Zerumbone (ZER) is a potential anticancer natural compound, isolated from Zingiber zerumbet Smith. In this investigation, the anticancer properties of ZER were evaluated on cancer cells of T-acute lymphoblastic leukemia, CEM-ss. The results showed that ZER has cytotoxic effect against CEM-ss cells with an IC(50) of 8....
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2010.07.025
更新日期：2011-02-01 00:00:00
abstract：:Normal human peripheral blood polymorphonuclear leukocytes (PMNs) and cells from a human acute promyelocytic leukemia line (HL-60) were tested for cytotoxic potential against two human glioma and one normal fibroblast line. Both the PMNs and HL-60 exhibited significant cytotoxicity against the tumor targets while spar...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(84)90101-2
更新日期：1984-01-01 00:00:00
abstract：:Cases of leukemia associated with Turner syndrome (TS) are rare. Here we report three TS patients with leukemia including one case of T-large granular lymphocyte leukemia (T-LGL), one rare case of coexistence of chronic lymphocytic leukemia (CLL) and idiopathic myelofibrosis (IMF) and one case of a patient with AML-M2...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2007.06.004
更新日期：2008-03-01 00:00:00
abstract：:Increased DNA damage and alteration of the DNA damage response (DDR) are critical features of genetic instability presumably implicated in pathogenesis of myelodysplastic syndromes (MDS) and acute myeloid leukemias (AML). We used immunofluorescence staining of γH2AX and 53BP1 for analyzing DNA double-strand breaks (DS...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2017.03.011
更新日期：2017-06-01 00:00:00
abstract：BACKGROUND:Little is known on racial differences in patients with diffuse large B-cell lymphoma (DLBCL). The aim of this retrospective study is to compare characteristics, prognostic factors and outcomes of Asian and Western patients with DLBCL treated with rituximab, cyclophosphamide, doxorubicin, vincristine and pred...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.01.002
更新日期：2013-04-01 00:00:00
abstract：:The monoclonal antibody AGF2.3 identifies a nuclear envelope protein expressed at high levels in most transformed cell lines and weakly expressed in or absent from normal tissues and primary cell cultures. Here we report that expression of AGF2.3 antigen relates to the maturation status of cells. When HL60 cells were ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(86)90235-3
更新日期：1986-01-01 00:00:00
abstract：:A case of acute monocytic leukemia (AMoL) with t(5;11)(q31;q23) and a case of acute myelomonocytic leukemia (AMMoL) with t(5;11)(q35;q13.1) are reported. The translocation between the long arm of chromosome 11q and that of chromosome 5q with leukemia have been rarely reported. Though breakpoint of both cases were subt...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(99)00033-8
更新日期：1999-07-01 00:00:00
abstract：:To improve the recovery rate of high-risk patients with acute promyelocytic leukemia (APL), we used all-trans retinoic acid (ATRA)/arsenic trioxide (ATO)/daunorubicin combination in remission induction, daunorubicin and cytarabine in consolidation, and ATRA/ATO/methotrexate ± 6-mercaptopurine in maintenance treatment ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.03.027
更新日期：2012-07-01 00:00:00
abstract：:We analyzed the function of a SEPT9-ABL1 fusion identified in a case of T-prolymphocytic leukemia with tyrosine kinase inhibitor (TKI) resistance. Five isoforms with different N-termini, including SEPT9a-ABL1, SEPT9b-ABL1, SEPT9d-ABL1, SEPT9e-ABL1 and SEPT9f-ABL1, were detected in the leukemic cells. All isoforms exce...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.08.015
更新日期：2014-12-01 00:00:00
abstract：:To examine the relationship between viability of acute myelogenous leukemia (AML) blasts in liquid suspension culture and the occurrence of myeloid maturation in culture, total and differential cell counts and viability as determined by trypan blue dye exclusion were followed over 21 days of culture. A significant pos...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(88)90098-7
更新日期：1988-01-01 00:00:00
abstract：BACKGROUND:The skin is the common extranasal sites in extranodal natural killer (NK) / T - cell lymphoma, (ENKTL). However, data regarding cutaneous ENKTL are relatively scarce. Thus, we performed a large retrospective study to analyze clinical features and treatment outcomes of cutaneous ENKTL. PATIENTS AND METHODS:S...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2019.106284
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:Chronic Myeloid Leukemia (CML) is characterized by the overproduction of BCR-ABL, a tyrosine kinase with constitutive activity, in which the majority of CML patients have e13a2 or e14a2 transcripts. Reckoned the possible associations between the hematologic and molecular features of the disease, a profound u...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2021.106512
更新日期：2021-01-19 00:00:00
abstract：:As a part of our continuing efforts to develop gene therapy for acute myelogenous leukemia (AML), this study was undertaken to evaluate the possibility of using autologous bone marrow stromal fibroblasts (BMSFs) as a target cell population. Autologous BMSFs in AML were isolated from the stromal layers of long-term bon...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(01)00134-5
更新日期：2002-04-01 00:00:00
abstract：:Twenty-three patients with acute myelogenous leukemia (AML) in first relapse were treated with high-dose cytosine-arabinoside (Ara-C) and amsacrine or idarubicin. To prime the cells, the patients were given rhGM-CSF. We studied the influence of 48-h infusion of rhGM-CSF on proliferation and Ara-C sensitivity of leukem...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/0145-2126(96)00005-7
更新日期：1996-06-01 00:00:00
abstract：:During a 4-yr period, 292 patients with acute leukaemia were studied using morphology, cytochemistry and immunologic reagents to determine the cell lineage of the leukaemia. One hundred and sixty-three cases were shown to be acute lymphoblastic leukaemia (ALL), 127 acute myeloblastic leukaemia (AML) and two cases (0.6...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(88)90002-1
更新日期：1988-01-01 00:00:00
abstract：:Resistance to chronic myeloid leukemia (CML) drug STI571 has been associated with point mutations in the kinase domain of BCR-ABL. For example, the mutation T315I (g. 68721C>T) and, to a lesser extent, Y253F (g. 58796A>T) appear in a significant proportion of patients resistant to treatment. Mutations appear intimatel...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(03)00066-3
更新日期：2003-11-01 00:00:00
abstract：:Ten patients with acute nonlymphocytic leukemia (ANLL) received bromodeoxyuridine (BrdU) at 100 mg/M2 intravenously over 1 h. BrdU is incorporated into the DNA by S-phase cells and was detected by using a monoclonal anti-BrdU antibody in the bone marrow aspirate (BM) and biopsy specimens obtained at the end of the inf...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90160-3
更新日期：1987-01-01 00:00:00
abstract：:Thirty patients with high-risk myelodysplastic syndrome, defined as > 5% bone marrow blasts, were treated with a combination of oral all-trans-retinoic acid (45 mg/m2 daily) and subcutaneous AraC (10 mg/m2) on days 1-14 of each 28-35 day cycle repeated for 2-6 cycles. Complete remission lasting 9, 12, and 15 months wa...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/s0145-2126(99)00095-8
更新日期：1999-09-01 00:00:00
abstract：:We describe the properties of three monoclonal antibodies (McAbs) (21H73, 37G7 and 49C12) against K562 cell surface antigens correlated with differentiation induced by 12-O-tetradecanoyl phorbol-13-acetate (TPA). Each of the McAbs immunoprecipitated K562 cell surface antigen with molecular weight (MW) of approximately...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90121-9
更新日期：1991-01-01 00:00:00
abstract：:We report the characterization of a novel human T-cell line, HPB-MLp-W, which was established from blastic cells of a lymph node specimen from a patient with non-Hodgkin's lymphoma. They demonstrated the T-cell association antigens, CD2 and CD4, but no CD3, CD8, CD1, CD5, CD7 nor T-cell antigen receptor on their cell ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90014-k
更新日期：1991-01-01 00:00:00
abstract：:The aims were to investigate the feasibility of imatinib mesylate (IM) discontinuation in chronic myeloid leukemia patients who were initially treated with IM and achieved complete molecular response (CMR). Fourteen patients were included. Ten were relapsed within 9.5 months after discontinuation of IM. All 7 patients...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2012.02.011
更新日期：2012-06-01 00:00:00
abstract：:Hyperdiploid and non-hyperdiploid multiple myeloma represents distinct biological entities characterized by different patterns of genetic changes. We sought to determine whether ploidy category (non-hyperdiploid versus hyperdiploid) remains stable over time from diagnosis to progression. Of the 43 patients studied (39...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2005.07.004
更新日期：2006-03-01 00:00:00
abstract：:The frequency of chromosomal alterations was compared among four children groups: those with Down syndrome and acute leukemia (DS/AL), those with acute leukemia (AL), those with only Down syndrome (DS) and healthy children (NC). The frequency of acquired chromosome abnormalities was larger in the AL group, followed by...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.03.015
更新日期：2005-11-01 00:00:00
abstract：:We present our first experiences with determination of minimal residual disease (MRD) based on patient specific Flt3-ITD (internal tandem duplication) mutations. We analysed MRD status of 11 AML patients in a retrospective investigation and its potential impact on the follow up of these patients. In five out of six pa...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2004.12.001
更新日期：2005-07-01 00:00:00
abstract：:Serial samples of peripheral blood were obtained from 75 children with acute lymphoblastic leukemia in remission. An immunofluorescence assay was used to quantitate TdT-containing (terminal deoxynucleotidyl transferase-containing) cells in the mononuclear leukocyte fraction of these specimens. Nine relapses in 8 patie...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90089-0
更新日期：1987-01-01 00:00:00
abstract：:Murine erythroleukemia (MEL) cells undergo erythroid differentiation in vitro when treated with hexamethylene bisacetamide (HMBA). To identify genes involved in the commitment of MEL cells to differentiate, we screened a cDNA library constructed from HMBA-induced cells by differential hybridization and isolated GTPase...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(02)00231-x
更新日期：2003-07-01 00:00:00
abstract：:Like CD19 or CD56, CD22 in non-lymphoid leukemia has been considered an aberrantly expressed antigen. CD22 had been believed to be restricted to B lymphoid, however, it was also found to be expressed in human basophils. Mature basophils are unique granulocytes expressing CD13, CD22 and CD25. To estimate whether the ex...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2003.10.034
更新日期：2004-07-01 00:00:00
abstract：:It is generally accepted that morphologically recognizable bone marrow (BM) cells are derived from progenitor cells committed to a specific line of haematopoietic differentiation [51]. The origin and morphological identity of such progenitor cells is not yet known and the question, whether there is a single pluripoten...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(85)90130-4
更新日期：1985-01-01 00:00:00
