Abstract:
:As a part of our continuing efforts to develop gene therapy for acute myelogenous leukemia (AML), this study was undertaken to evaluate the possibility of using autologous bone marrow stromal fibroblasts (BMSFs) as a target cell population. Autologous BMSFs in AML were isolated from the stromal layers of long-term bone marrow culture (LTBMC) using immunomagnetic beads. BMSFs exhibited rapid proliferation even in the absence of growth factors. Cultures stimulated with bFGF produced significantly increased numbers of BMSFs than cultures without added growth factors. Using LNC/LacZ retroviral vector, the transduction efficiency of BMSFs was 13+/-4% at a 5 multiplicity of infection (MOI). LNC/interleukin-2 (IL-2)-transduced BMSFs produced between 1200 and 4800pg of IL-2/10(6) cells per 24h. Using adenoviral vector AdV/LacZ, the transduction efficiency was 84+/-10% at 100, and 92+/-8% at a MOI of 1000. Although the addition of basic fibroblast growth factor, epidermal growth factor, or platelet-derived growth factor did not affect the transduction efficiency, they increased the numbers of transduced cells significantly (P<0.01). AdV/IL-2-treated BMSFs produced high levels of IL-2 over the course of 7 days between 9820 and 22,700pg of IL-2/10(6) cells per 24h. Our finding that the genetically engineered autologous BMSFs of AML could be successfully established in vitro implies that BMSFs obtained from LTBMC might be considered as a target cell population for certain types of clinical gene therapy in AML.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Min YH,Li GX,Jang JH,Suh HC,Kim JS,Cheong JW,Lee ST,Hahn JS,Ko YWdoi
10.1016/s0145-2126(01)00134-5keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
369-76issue
4eissn
0145-2126issn
1873-5835pii
S0145212601001345journal_volume
26pub_type
杂志文章abstract::Leukemic cell lines have become important tools for studies of disease providing a monoclonal cell population that can be extensively expanded in vitro while preserving leukemic cellular characteristics. However, studies of chronic lymphocytic leukemia (CLL) have been impeded in part by the lack of continuous human ce...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2015.10.008
更新日期:2016-01-01 00:00:00
abstract::Recent studies indicate that lipid metabolic changes affect the survival of multiple myeloma (MM) cells. Time-of-flight secondary ion mass spectrometry (TOF-SIMS), an imaging mass spectrometry technique, is used to visualize the subcellular distribution of biomolecules including lipids. We therefore applied this metho...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2015.02.011
更新日期:2015-06-01 00:00:00
abstract::The effect of prostaglandin E2 (PGE2) on the reduction of c-myc expression during the differentiation of the human leukemic cell line, HL-60, was examined. PGE2, a potent inducer of intracellular cyclic AMP (cAMP) in HL-60 cells, augmented monocyte-associated cell surface antigens induced by human gamma-interferon (IF...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(88)90090-2
更新日期:1988-01-01 00:00:00
abstract::Leukotrienes and lipoxins are bioactive lipoxygenase products formed by leukocytes alone or in collaboration with other cells. While the physiological role of lipoxins remains to be clarified, accumulating evidence shows that leukotrienes are important mediators in asthma and inflammation. Consequently, recent clinica...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/0145-2126(94)90053-1
更新日期:1994-10-01 00:00:00
abstract::Despite high rates of hematologic and cytogenetic responses to imatinib therapy, the emergence of resistance to imatinib has been recognized as a major problem in the treatment of Ph-positive leukemia. The high frequency of BCR-ABL mutations and amplifications represents the high degree of heterogeneity in patients wi...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2003.10.007
更新日期:2004-05-01 00:00:00
abstract::Rhodamine 123 is transported by the transmembrane efflux pump P glycoprotein (Pgp). We used this fluorescent dye to study multidrug resistance (MDR) activity in normal and leukemic CD34+ cells. These immature cells had a high degree of MDR activity. Among leukemic cells, CD34+ leukemias had significantly higher MDR ac...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(93)90159-i
更新日期:1993-12-01 00:00:00
abstract::We evaluated the molecular mechanism of telomerase activation by erythropoietin (EPO) in human erythroleukemic JAS-REN-A cells. Telomerase activity increased 3-4 fold after 3-24h of culture with EPO and was associated with increases in c-myc mRNA after 1-3h, of c-Myc protein after 3-6h, and of human telomerase reverse...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.11.002
更新日期:2011-03-01 00:00:00
abstract::We have recently established the MV4-11 acute myelogenous leukemia (AML) subline, designated as MV4-11 TP53 R248W, which possesses a missense mutation (CGG→TGG; R248W) in the TP53 gene, leading to inactivation of this transcription factor. DNA methyltransferase (DNMT) inhibitor 5-Aza-2'-deoxycytidine (5-AzadC) induced...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2011.04.004
更新日期:2011-07-01 00:00:00
abstract::The efficiency of gene transfer into human leukemia cell lines by electroporation was investigated. For both transient expression (beta-galactosidase gene) and stable transformation (neomycin resistance gene), the transfer efficiency into leukemia cell lines using a square wave pulse was superior to that using an expo...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(91)90062-x
更新日期:1991-01-01 00:00:00
abstract::Early T-cell precursor-ALL (ETP-ALL) is a subtype of T-ALL with a poor prognosis in children. We analyzed ETP-ALL compared to conventional T-ALL/LBL in both adults and children to determine any differences in clinical outcomes, based on the following parameters: induction failure, relapse, and survival. Patients with ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.06.010
更新日期:2013-09-01 00:00:00
abstract::We report single institution outcome of brief, intensive ara-C-based chemotherapy using bone marrow transplantation as primary intensification for untreated adult patients with acute lymphoblastic leukemia (ALL). Overall disease-free and overall survival were inferior to those reported with prolonged chemotherapy mode...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00175-8
更新日期:2002-05-01 00:00:00
abstract:OBJECTIVE:Bortezomib-dexamethasone-thalidomide has been reported to be effective in newly-diagnosed multiple myeloma (MM) with an overall response rate of 92% and a CR rate of 18% (Alexanian et al, Hematology 2007;12(3):235-9), but this regimen has not been tested in the Chinese patients. We report here our results tes...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.04.006
更新日期:2009-12-01 00:00:00
abstract::To clarify whether expression of the programmed cell death 5 (PDCD5) gene in leukemic cells is abnormal, real-time quantitative reverse transcription polymerase chain reaction (RQ-RT-PCR) was used to examine its expression in marrow cells from leukemia patients. We found lower PDCD5 in both AML and CML marrow cells th...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.12.028
更新日期:2006-09-01 00:00:00
abstract::Nasal-type natural killer/T-cell (NK/T-cell) lymphomas are subtypes of non-Hodgkin's lymphoma (NHL), which are typically more clinically aggressive. There is, however relatively little understanding of nasal-type NK/T-cell lymphoma molecular pathogenesis. Thus, in this study we applied RNA sequencing to systematically...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2015.10.001
更新日期:2015-12-01 00:00:00
abstract::Primary cardiac lymphoma (PCL) is a rare and usually fatal neoplasm, which may cause syncope, arrhythmia, heart failure and pericardial effusion as presenting clinical complaints. A case of PCL in a 72-year-old man with moderate aortic stenosis is presented. The patient was investigated because of pericardial effusion...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/s0145-2126(01)00092-3
更新日期:2002-01-01 00:00:00
abstract::Peripheral blood and/or bone marrow lymphoblasts from 25 patients with acute lymphoblastic leukemia (ALL) were tested with a panel of monoclonal antibodies (MoAbs) and conventional hematopoietic markers by three different laboratories. The results were analysed to evaluate the reproducibility of ALL phenotype determin...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(86)90083-4
更新日期:1986-01-01 00:00:00
abstract::Migration of eosinophil (Eo) into tissues is a hallmark of chronic eosinophilic leukaemia (CEL), but the exact mechanism involved in cell migration is unknown. We report on a patient with CEL who presented high expressions of VLA-4, LFA-1 and Mac-1 integrins on the Eo surface, increased chemotaxis of Eo to eotaxin, de...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.07.025
更新日期:2007-05-01 00:00:00
abstract::We examined the antineoplastic effects of the iron chelators, deferasirox and deferoxamine in multiple myeloma cell lines as well as primary myeloma cells. These iron chelators showed marked antiproliferative activity as well as cytotoxicity toward myeloma cell lines and deferasirox was cytotoxic to bone marrow plasma...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2014.06.005
更新日期:2014-08-01 00:00:00
abstract::We investigated the accumulation of DNA strand breaks in a human promyelocytic leukemia cell line, HL-60, treated with methotrexate (MTX) and 1-beta-D-arabinofuranosylcytosine (Ara-C). The sequential treatment with MTX then Ara-C had a synergistic effect on the formation of DNA strand breaks, which was dependent on MT...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90139-2
更新日期:1989-01-01 00:00:00
abstract::To evaluate the clinical usefulness of interleukin 2 (IL-2) on myelodysplastic syndromes (MDS), the serum IL-2 level, the effect of IL-2 on the proliferation of blasts, and the cell-mediated cytotoxic effect of IL-2 on blasts were examined in MDS patients. Of 18 patients, 2 patients had an increased serum IL-2 level. ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(93)90058-s
更新日期:1993-02-01 00:00:00
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journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(86)90251-1
更新日期:1986-01-01 00:00:00
abstract::In this study, we estimated the association between the inferred haplotypes in the inflammation, DNA repair, and folate pathways, and developed risk models for Hodgkin lymphoma. The study population consisted of 200 Hodgkin lymphoma cases and 220 controls. A susceptible association was observed on the XPC gene with ha...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.07.014
更新日期:2012-11-01 00:00:00
abstract::The MYBL2 gene encodes a transcription factor implicated in cell proliferation and maturation whose amplification or overexpression has been associated with different human malignancies, suggesting that it could be implicated in tumorigenesis. We analyzed MYBL2 expression and its prognostic value in 291 patients with ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.09.015
更新日期:2013-12-01 00:00:00
abstract::Hodgkin and Sternberg Reed (H and SR) cells, the putative malignant cells of Hodgkin's disease carry regularly T-cell activation antigens, like CD30 and CD25 (low affinity IL-2 receptor). We have investigated the Hodgkin cell line L540, bearing characteristic markers of H and SR cells for its expression of the low aff...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(90)90107-k
更新日期:1990-01-01 00:00:00
abstract::Bone marrow cells from 9 patients with acute myeloid leukemia and 1 patient with a blast crisis of chronic myeloid leukemia were cultured to determine their ability to be induced to differentiate by different chemotherapeutic compounds. Five of these 10 patients showed differentiation to granulocytic and/or monocytic ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(85)90087-6
更新日期:1985-01-01 00:00:00
abstract::The aim of this study is to detect the biologic and/or prognostic significance of survivin (S) and effector protease receptor 1: EPR-1 (E) expression in acute leukemias (34 ALL and 40 AML) by using RT-PCR. S and E expressions were found in 83.8 and 20.3% of the cases, respectively. S was detected in 90%, 76.5% and E w...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.01.005
更新日期:2007-11-01 00:00:00
abstract::Philadelphia-positive chronic myeloid leukemia (Ph+ CML) patients who are resistant to imatinib are commonly treated with second-generation tyrosine kinase inhibitors (TKIs). Limited data exist on the possible effects of these drugs on subsequent allogeneic hematopoietic stem cell transplantation (allo-HSCT).The outco...
journal_title:Leukemia research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.leukres.2009.04.036
更新日期:2010-02-01 00:00:00
abstract::The use of low density lipoprotein (LDL) as drug carrier in acute myeloblastic leukemia chemotherapy is attractive due to high LDL uptake by leukemic cells. Lipid-based formulations, such as liposomes or microemulsions are promising alternatives. In the current study, we incorporated N-trifluoroacetyl-adriamycin-14-va...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.06.012
更新日期:2006-02-01 00:00:00
abstract::Twenty-three patients with acute myelogenous leukemia (AML) in first relapse were treated with high-dose cytosine-arabinoside (Ara-C) and amsacrine or idarubicin. To prime the cells, the patients were given rhGM-CSF. We studied the influence of 48-h infusion of rhGM-CSF on proliferation and Ara-C sensitivity of leukem...
journal_title:Leukemia research
pub_type: 临床试验,杂志文章
doi:10.1016/0145-2126(96)00005-7
更新日期:1996-06-01 00:00:00
abstract::The effects of two recombinant human CSFs (G-CSF and GM-CSF) on the growth of blast progenitors from 36 acute myeloblastic leukemia patients were studied in methylcellulose and suspension cultures. Blast colony formation in methylcellulose and the growth of blast progenitors in suspension were stimulated by G-CSF or G...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(88)90063-x
更新日期:1988-01-01 00:00:00