Abstract：
:Resistance to chronic myeloid leukemia (CML) drug STI571 has been associated with point mutations in the kinase domain of BCR-ABL. For example, the mutation T315I (g. 68721C>T) and, to a lesser extent, Y253F (g. 58796A>T) appear in a significant proportion of patients resistant to treatment. Mutations appear intimately related to the development of resistance, and they may pre-exist in a small percentage (<1%) of tumor cells at the time of treatment initiation. Most mutation detection methods, including sequencing, are unable to detect such a small percentage of mutations in a background of wild type sequences. We describe the simplification and modification of a recently developed enhanced PCR-RFLP method, and its application to the detection of T315I and Y253F mutations. The method is quantitative, can be used in agarose gel or real time PCR formats, and reliably detects 1 mutation-containing cell in a background of almost 1000 non-mutated cells. The increased sensitivity offered by this assay will allow detection of these mutations at an early stage during treatment and will be useful in rational treatment modification and in studies which address the association between these mutations and drug resistance.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Liu WH,Makrigiorgos GMdoi
10.1016/s0145-2126(03)00066-3keywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
979-82issue
11eissn
0145-2126issn
1873-5835pii
S0145212603000663journal_volume
27pub_type
杂志文章abstract：:Cell line PER-423 was derived from the cells of a patient with an immature acute T-lymphoblastic leukaemia and the growth of this human cell line is strictly dependent on interleukin-2 (IL-2). PER-423 cells express the p75 (beta) subunit of the IL-2 receptor (IL-2R beta), while the p55 chain (IL-2R alpha) is not detec...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(93)90141-7
更新日期：1993-01-01 00:00:00
abstract：:A 53-year-old male patient was admitted to our hospital with painful splenomegaly. He was diagnosed as having chronic myelomonocytic leukemia (CMML) with leukocytosis, monocytosis, increased lysozyme concentrations in serum und urine, and lack of the Philadelphia chromosome. The clinical course of the disease was char...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(96)00117-8
更新日期：1997-03-01 00:00:00
abstract：:We have investigated the production of metalloproteinases (MMP-1, MMP-2, MMP-9) and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) in bone marrow stromal cells (BMSCs) of patients with multiple myeloma (MM) and a healthy control. The new findings of this paper is that BMSCs of the MM patients exhibited in...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.04.001
更新日期：2008-11-01 00:00:00
abstract：:Mantle cell lymphoma (MCL) is an aggressive malignancy and new treatment modalities must be established to increase patient survival time. In the search for new therapeutic targets, reliable and well-characterised in vitro models are essential. In this study, we have characterised three MCL cell lines (SP53, Granta 51...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2004.06.009
更新日期：2005-02-01 00:00:00
abstract：:An overview is given of the effects of X-irradiation, ovariohysterectomy and estradiol-17 beta administration on mammary tumorigenesis in females of 3 rat strains, viz. the WAG/Rij, BN/BiRij and SD. The 3 rat strains differed significantly in their spontaneous mammary tumor incidence. Female SD rats had the highest in...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(86)90292-4
更新日期：1986-01-01 00:00:00
abstract：:Prospective data are needed to ascertain the impact of iron chelation therapy in patients with myelodysplastic syndromes. The present 5-year prospective registry analysis was conducted to compare clinical outcomes between chelated and nonchelated patients with lower-risk myelodysplastic syndromes and transfusional iro...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2017.01.033
更新日期：2017-05-01 00:00:00
abstract：:Occupational and industrial exposures have been implicated in the etiology of leukemia, yet uncertainty remains regarding potential high risk occupations. We examined the associations between self-reported occupations and hobbies and acute leukemia risk using data from 811 cases and 637 controls participating in a cas...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2005.03.002
更新日期：2005-10-01 00:00:00
abstract：:We report on four cases of trisomy 14 as the sole anomaly. Three cases were myelodysplastic syndromes and one was a non-Hodgkin's lymphoma. This anomaly is mainly in myeloid disorders and still remains to be well documented. On the other hand, we show this anomaly to be also a non-random anomaly in lymphoproliferative...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(92)90181-6
更新日期：1992-01-01 00:00:00
abstract：:Brazil is a wide country with huge contrasts. Its peculiarities can highlight environmental factors that could influence the frequencies of different cancers. The standard treatment and results achieved from several different areas of the country may not be found in others. The establishment of a national cooperative ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(01)00187-4
更新日期：2002-07-01 00:00:00
abstract：:This study uses single cell network profiling (SCNP) to characterize biological pathways associated with in vitro resistance or sensitivity to chemotherapeutics commonly used in acute myeloid leukemia (AML) (i.e. cytarabine/daunorubicin, gemtuzumab ozogamicin (GO), decitabine, azacitidine, clofarabine). Simultaneous m...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.02.022
更新日期：2012-07-01 00:00:00
abstract：:Despite initial remissions, most patients with Ph chromosome positive (Ph(+)) acute leukemia (AL) become refractory to tyrosine kinase inhibitors (TKIs) such as imatinib and dasatinib. This study was designed to determine if targeting the interleukin-3 receptor (IL-3R) with a diphtheria toxin fusion protein (DT(388)IL...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.12.008
更新日期：2010-08-01 00:00:00
abstract：:Promoter hypermethylation occurs early in leukemogenesis and seems to be associated with poor prognosis in acute lymphoblastic leukemia (ALL). The methylation status of the promoter region of six genes was analyzed in 71 children with ALL using methylation specific PCR (MSP). Calcitonin (CALCA) and E-cadherin (CDH1) w...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.11.016
更新日期：2006-07-01 00:00:00
abstract：:The involvement of histone lysine methyltransferases (HMT) in carcinogenesis is not well understood. Here, we describe a dose-dependent growth and survival inhibitory effects of BIX-01294, a specific inhibitor of euchromatic HMT2, in promyelocytic leukemia HL-60 and NB4 cells. BIX-01294 combined with all-trans retinoi...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.04.003
更新日期：2014-07-01 00:00:00
abstract：:Concern has been expressed recently about apparent increases in the incidence of leukaemia amongst young people living in certain geographical areas. We analysed the incidence of childhood leukaemia in South-East Scotland (excluding North-East Fife) from 1970 to 1984. There was a significant geographical variation in ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(86)90091-3
更新日期：1986-01-01 00:00:00
abstract：:During a 4-yr period, 292 patients with acute leukaemia were studied using morphology, cytochemistry and immunologic reagents to determine the cell lineage of the leukaemia. One hundred and sixty-three cases were shown to be acute lymphoblastic leukaemia (ALL), 127 acute myeloblastic leukaemia (AML) and two cases (0.6...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(88)90002-1
更新日期：1988-01-01 00:00:00
abstract：:The effect of serum on the proliferation of human bone marrow pluripotent progenitor cells (CFU-GEMM), was compared to that of fresh frozen plasma (FFP). Serum significantly increased the number of mixed erythroid-granulocytic-megakaryocytic colonies (CFU-GEMM) and erythrocytic bursts (BFU-E) in this assay system (p l...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(85)90062-1
更新日期：1985-01-01 00:00:00
abstract：:The sum of endogenous and food-derived antioxidants provides an estimate of the total antioxidant capacity (TAC) of the extracellular fluids, while corrected TAC (cTAC) is an estimation of the exogenously provided antioxidants. Similar values for TAC and cTAC were observed between cancer free children and children wit...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2004.04.017
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:A consecutive series of acute myeloid leukemias (AML) patients was analyzed in conditions which reduce the inter-assay variations (the same flow cytometer, the same observers and the same panel of monoclonal antibodies) in order to investigate the prognostic information provided by flow cytome...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2005.02.015
更新日期：2005-10-01 00:00:00
abstract：:Although clinical experience and in vitro data provide evidence of an anti-leukemic activity of T cells, there are few examples of recognition of leukemic cells by tumor-specific T cells in vitro. Tumor antigens encoded by the MAGE genes are useful tools to study this recognition. We tested the sensitivity to recognit...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(00)00053-9
更新日期：2000-10-01 00:00:00
abstract：:Radiotherapy (RT)is considered the treatment of choice in patients with Extra-nodal marginal zone lymphoma (EMZL) at early stage, but the presence of late toxicities has been limited the acceptance. Recently, low doses of RT LDR) (2 x 2 Gy) and the use of limited fields has been observed that retain the efficacy but e...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2020.106443
更新日期：2020-11-01 00:00:00
abstract：:Heterogeneity for 5'nucleotidase has been demonstrated in chronic lymphocytic leukemia [12]. The enzyme is not detectable in the lymphocytes from the majority of patients with this disorder, but normal and even supranormal levels are found in some cases [17]. In the present studies, the properties of this ecto-nucleot...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(82)90026-1
更新日期：1982-01-01 00:00:00
abstract：:Predisposition to lymphomagenesis is a well-known phenomenon of ataxia-telangiectasia, a recessive disorder caused by germline inactivation of ATM. ATM encodes a protein implicated in the repair of radiation induced double-strand breaks. Biallelic ATM inactivation was described also in sporadic lymphoid malignancies, ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.11.010
更新日期：2006-09-01 00:00:00
abstract：:Acute myeloid leukemia (AML) is frequently treated with induction and consolidation chemotherapy. Consolidation chemotherapy can be delivered on an ambulatory basis, requiring some patients to travel long distances for treatment at specialized centers. We developed a shared care model where patients receive consolidat...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2017.05.023
更新日期：2017-08-01 00:00:00
abstract：:Diagnosis of MDS has changed during the last years because of the 2008 WHO classification. Complete blood cell count (CBC) is a very important tool both for diagnosis of MDS. The aim of this study was to evaluate if it is possible to use the abnormal signals produced by dysplastic cells to produce a flag "dysplasia" a...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.06.015
更新日期：2011-12-01 00:00:00
abstract：:Febrile neutropenia (FN) risk-assessment and granulocyte-colony stimulating factor (G-CSF) prophylaxis use in clinical practice was evaluated in patients with diffuse large B-cell lymphoma receiving R-CHOP-21. More G-CSF primary prophylaxis was used in patients assessed as high FN risk, but R-CHOP-21 was associated wi...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.02.002
更新日期：2012-05-01 00:00:00
abstract：BACKGROUND:Therapy strategies for myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) vary considerably. OBJECTIVE:To review the treatment of Brazilian children who were diagnosed with MDS or JMML in the past decade and reported to the Brazilian Cooperative Group on Pediatric Myelodysplastic Sy...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2004.01.008
更新日期：2004-09-01 00:00:00
abstract：:We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3Ralpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was promi...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.03.047
更新日期：2009-09-01 00:00:00
abstract：:Defective apoptosis is a hallmark of the progression of B chronic lymphocytic leukaemia (B-CLL). Smac-mimetics have been shown to induce apoptosis in several tumours. We describe the in vitro pro-apoptotic activity and regulation of the molecular pathway induced by new Smac-mimetics in B-CLL. The cytotoxic effect was ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.03.016
更新日期：2013-07-01 00:00:00
abstract：:Epstein-Barr virus (EBV) can induce a broad spectrum of hematological diseases, especially in immune deficient patients. We assayed for receptor for EBV (EBVR) using fluoresceinated viral particles on 44 human hematopoietic cell lines derived from patients with T, B, and non-T, non-B acute lymphocytic leukemia (ALL), ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(85)90085-2
更新日期：1985-01-01 00:00:00
abstract：:The ubiquitin-proteasome pathway is implicated in the pathogenesis of many haematologic malignancies, including multiple myeloma. Under conditions of rapid cell turnover and growth rate, proteasomes are returned into circulation. The measurement of their levels or activity could offer a new approach to diagnosis, prog...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.05.008
更新日期：2014-08-01 00:00:00
