Abstract：
:Mantle cell lymphoma (MCL) is an aggressive malignancy and new treatment modalities must be established to increase patient survival time. In the search for new therapeutic targets, reliable and well-characterised in vitro models are essential. In this study, we have characterised three MCL cell lines (SP53, Granta 519 and NCEB1) in comparison with primary tumours from MCL, follicular lymphomas (FL), a FL cell line (RL), a Burkitt lymphoma cell line (RAJI) and five different B cell populations from healthy individuals. Expression profiling was used to determine the relative expression of >12000 transcripts in these samples, and flow cytometry analysis was performed to establish a phenotypic signature for each of the cell lines. In addition, the cell lines were sequenced, and the frequency of somatic mutations and immunoglobulin (Ig) variable heavy chain (VH) usage were determined. We show by hierarchical clustering that the cell lines retain a genetic signature similar to primary MCL, which readily separated the MCL samples from the other lymphoma cell lines and the FL tumours. Furthermore, the MCL cell lines showed differences in the frequency of VH somatic mutations (0-2.1%). The increased number of mutations in NCEB1, compared to the other MCL cell lines, was in agreement with a decreased expression of CD31, CD44, CXCR5, CCR7 and CCR6. Taken together, our data show that the cell lines are clearly derived from MCL tumours and expressed similar genetic and phenotypic signatures compared to primary tumours, which confirmed their usefulness as in vitro models.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Ek S,Ortega E,Borrebaeck CAdoi
10.1016/j.leukres.2004.06.009keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
205-13issue
2eissn
0145-2126issn
1873-5835pii
S0145-2126(04)00239-5journal_volume
29pub_type
杂志文章abstract：:During monocyte-macrophage differentiation of HL-60 cells by 12-O-tetradecanoyl phorbol 13-acetate, the intracellular globular(G)-actin and polymerized(F)-actin increased 3-fold and 1.7-fold, respectively. Time course studies showed that these changes of actin levels were nearly coincident with the development of macr...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(89)90121-5
更新日期：1989-01-01 00:00:00
abstract：:Fractionation of mononuclear cells from human fetal liver provides a cell population at early stages of myeloid differentiation which, when cultured, generates neutrophils and macrophages for up to a month. These studies describe the further purification of an undifferentiated myeloid blast cell population by rosette ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(90)90114-o
更新日期：1990-01-01 00:00:00
abstract：:When cells were incubated in the presence of both interferon-gamma (IFN-gamma) and all-trans retinoic acid (ATRA), the concentration of IFN-gamma required to induce apoptosis of B-cell lymphoma cells was much lower than that required for myeloid or erythroid cell lines. The concentration of IFN-gamma that effectively ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(01)00202-8
更新日期：2002-08-01 00:00:00
abstract：:High CD23 expression is a hallmark of B-CLL cells. It is lost during in vitro culture and can be reinduced by IL-4, albeit to a lower extent than in normal B cells. To elucidate the events controlling CD23 expression in B-CLL cells, the IL-4 mediated induction of STAT6 was investigated. Western-blot analysis demonstra...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(99)00191-5
更新日期：2000-04-01 00:00:00
abstract：:Despite the high effectiveness of various P-glycoprotein (P-gp) modulating substances in vitro their clinical value e.g. for combination treatment of acute myelogenous leukemias (AML) remains still unclear. This might be explainable by recent findings that other factors than P-gp (e.g. the multidrug resistance associa...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(98)00192-1
更新日期：1999-05-01 00:00:00
abstract：:Older patients with acute myelogenous leukemia (AML) have overexpression of P-glycoprotein (Pgp+), and this has been shown to correlate quantitatively with therapeutic outcome. Since Pgp-mediated efflux of cytotoxic drugs can be inhibited by the cyclosporine analogue, PSC 833, we investigated the use of this agent wit...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/s0145-2126(00)00024-2
更新日期：2000-07-01 00:00:00
abstract：:Although verapamil, cyclosporin A. cremophor EL and PSC-833 are active as multidrug resistance modulators, there has been limited study of these compounds as possible chemotherapy enhancing agents against drug-sensitive tumors. We compared these agents as modifiers of VP-16 cytotoxicity in vitro and modifiers of VP-16...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(95)00029-n
更新日期：1995-08-01 00:00:00
abstract：:In this study we investigated the Ara-CTP-forming capacity of leukemic cells in different phases of the cell cycle. Cells from two leukemic cell lines and leukemic bone marrow cells from patients and rats (BNML model) with acute myelocytic leukemia were separated according to cell cycle phase by means of an albumin de...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(90)90164-5
更新日期：1990-01-01 00:00:00
abstract：:Selective inhibition of the BCR/ABL tyrosine kinase by imatinib (STI571, Glivec/Gleevec) is the therapeutic strategy in patients with chronic myelogenous leukemia (CML). Despite significant hematologic and cytogenetic responses with imatinib, mainly due to the mutations in the Abl kinase domain, resistance occurs in p...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.11.007
更新日期：2008-06-01 00:00:00
abstract：:To highlight dasatinib role in the elderly, 125 unselected patients with CP-CML aged >60 years resistant/intolerant to imatinib were retrospectively evaluated. Grade 3-4 haematological and extra-haematological toxicities were reported in 39 (31.2%) and 34 (27.2%) patients; grade 3-4 haematological toxicity was higher ...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2011.05.015
更新日期：2011-09-01 00:00:00
abstract：:Two white European males are reported with adult T-cell leukemia (ATL), a disease first described in Japan, but recently also in the U.K. and U.S.A. Both patients presented with lymphadenopathy, but without a mediastinal mass. In addition, one patient had skin infiltrates and the other had hepatosplenomegaly. Morpholo...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(83)90055-3
更新日期：1983-01-01 00:00:00
abstract：:Nitric oxide (NO) has been shown to be cytotoxic for normal and transformed cell lines. One of the intracellular targets for NO action is glutathione (GSH). GSH determinates cellular redox potential and modulates several biological events. During oxidative and nitrosative stress, glutathione system imbalance is associ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.06.021
更新日期：2006-02-01 00:00:00
abstract：:Flow cytometry is commonly used to establish the diagnosis of chronic lymphocytic leukemia (CLL). A defined combination of antibodies discriminates between normal B cells and CLL cells (coexpression of CD5, CD19, and CD23). The receptor tyrosine-like orphan receptor one (ROR1) is an embryonic glycoprotein involved in ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.04.006
更新日期：2011-10-01 00:00:00
abstract：:We studied monosomy and deletions of chromosome 7 in 170 patients with myeloid disorders and we identified a minimal region of loss in 7q31.1 spanning between the D7S2554 and D7S2460 markers. The closest gene to our most deleted microsatellite, D7S2554, is the human I-mfa domain containing (HIC) gene, alias MyoD famil...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.09.007
更新日期：2007-04-01 00:00:00
abstract：:In AML, prevention of GvHD leads to better tolerance of myeloablative therapy. 66 individuals with AML in CR underwent myeloablative conditioning and transplantation with allogeneic PBPC grafts. Median presentation age was 44.5 years. Karyotyping was intermediate in 48% and of unfavourable risk in 36%. For GvHD prophy...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2011.06.039
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND/AIMS:Multiple myeloma (MM) is caused by proliferation of clonal plasma cells (cPCs) in bone marrow (BM), associated with numerical and functional defects in immune subsets. An impairment of B cell compartment is involved in onset/progression of the disease. METHODS:By flow cytometry, we studied distribution...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2016.08.008
更新日期：2016-10-01 00:00:00
abstract：:MDS is primarily a disease of the elderly. Cases who give a history of exposure to X-rays, cytotoxic drugs or leukaemogenic chemicals may be younger. Many cases of MDS present because of an incidental blood count. The most prominent clinical features are those of anaemia, neutropenia, thrombocytopenia. Because haemopo...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(92)90106-h
更新日期：1992-01-01 00:00:00
abstract：:Hematopoiesis is regulated by several cytokines with pleiotropic activity. Several evidences have clearly demonstrated that these molecules, formerly regarded as specific for the hematopoietic system, also affect certain endothelial cell functions and that hematopoietic factors clearly influence angiogenesis. This rev...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2012.02.003
更新日期：2012-05-01 00:00:00
abstract：:To further understand the role of XIAP in acute myeloid leukemia (AML), we suppressed XIAP expression by antisense oligonucleotides and determined the effect on gene expression profiles and biological pathways. XIAP inhibition upregulated expression of proteasome genes in a manner similar to the proteasome inhibitor b...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.04.018
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVES:Follicular lymphoma (FL) is generally considered an indolent disorder but a significant subset of patients shows a worse outcome. Aim of this study was to validate the FLIPI score in an independent series of follicular lymphoma patients and to correlate prognostic categories with the period of diagnosis and ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.07.006
更新日期：2006-03-01 00:00:00
abstract：:A double monoclonal component (MC) detected in the serum and/or urine represents a very rare occurrence (2-6% of monoclonal gammopathies). In this study, we report 34 patients with double serum MCs, focusing on the associated diseases. The diagnosis was made using high-resolution serum protein electrophoresis and immu...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.05.008
更新日期：2012-10-01 00:00:00
abstract：:We conducted a retrospective analysis to investigate the clinical outcome of combined-modality therapy using multiagent chemotherapy and involved-field radiotherapy in treatment of children with Hodgkin's lymphoma. Fifty eight cases with newly diagnosed Hodgkin's lymphoma were analyzed. The median follow-up duration w...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2010.06.012
更新日期：2010-11-01 00:00:00
abstract：:In vivo infection by HTLV-I is characterized by the lack of virus expression in the host's infected cells and in most cases it is clinically inapparent. The present study shows that exposure of HTLV-I-infected human T-cells to a maximal non-toxic dose of 3-MC remarkably enhances virus expression. This stimulatory effe...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(93)90098-6
更新日期：1993-05-01 00:00:00
abstract：:It has been suggested that acidic isoferritins play a major role in the regulation of human granulocyte-macrophage (CFU-GM) proliferation. Such regulatory isoferritins are said to occur in some human tissues including both leukaemic and normal leucocytes, and certain established cell lines. They are glycosylated and b...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(84)90065-1
更新日期：1984-01-01 00:00:00
abstract：:In acute lymphoblastic leukemia (ALL) patients, automated fluorescence fragment analysis (ALF) has been reported to improve the monoclonality detection rate of immunoglobulin heavy chain genes (IgH) polymerase chain reaction (PCR) analysis. This study performed complementary determining region (CDR) I and III PCR on s...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(01)00025-x
更新日期：2001-09-01 00:00:00
abstract：:Aggressive chemotherapy of myelodysplastic syndromes is rarely feasible because these disorders predominantly occur in elderly patients who often have concurrent illnesses. Alternative treatment modalities must therefore be evaluated. This review summarizes the results that have been obtained with low-dose chemotherap...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(92)90058-f
更新日期：1992-01-01 00:00:00
abstract：:IL-3 stimulated the production of IL-6 from a bone marrow-adherent cell population, macrophages and from hemopoietic supportive stromal cell lines. It also induced IL-6 production from a stem cell-enriched population of bone marrow cells which did not produce IL-6 without stimulation. In contrast, stimulation with IL-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90180-2
更新日期：1991-01-01 00:00:00
abstract：:Thirty patients with high-risk myelodysplastic syndrome, defined as > 5% bone marrow blasts, were treated with a combination of oral all-trans-retinoic acid (45 mg/m2 daily) and subcutaneous AraC (10 mg/m2) on days 1-14 of each 28-35 day cycle repeated for 2-6 cycles. Complete remission lasting 9, 12, and 15 months wa...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/s0145-2126(99)00095-8
更新日期：1999-09-01 00:00:00
abstract：:We have studied 35 adult patients with morphologically undifferentiated peroxidase-negative acute leukemia that failed to meet the criteria for acute lymphoblastic leukemia and compared them to patients with FAB M1-M7 seen by the same physicians. The diagnosis of minimally differentiated acute leukemia (MD-AL) was ass...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(93)90002-3
更新日期：1993-03-01 00:00:00
abstract：:This report describes a unique case of acute myeloid leukemia with hypergranular cytoplasm and t(X;11)(q24;q23). The breakpoint on 11q23 was identified within the MLL gene. The hypergranular cytoplasm of leukemic cells and the associated coagulopathy resembled a characteristic of acute promyelocytic leukemia, despite ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(98)00131-3
更新日期：1999-01-01 00:00:00
