Abstract：
:Despite the high effectiveness of various P-glycoprotein (P-gp) modulating substances in vitro their clinical value e.g. for combination treatment of acute myelogenous leukemias (AML) remains still unclear. This might be explainable by recent findings that other factors than P-gp (e.g. the multidrug resistance associated protein (MRP)) may also be involved in clinical occurring drug resistance. To study P-gp and MRP mediated MDR in AML blasts from patients with relapses at the functional level we measured rhodamine 123 (RHO) efflux in combination with a P-gp specific (SDZ PSC 833) or a MRP specific (MK571) modulator, respectively. Furthermore, direct antineoplastic drug action was monitored by determination of damaged cell fraction of a blast population using flow cytometry. We generally found strongly modulated RHO efflux by SDZ PSC 833 but slight RHO-efflux modulation by MK571 in blasts from relapsed states of AML expressing MDR1 or MRP mRNA at various levels. We could not demonstrate, though, significant PSC 833 or MK571 mediated modulation of the cytotoxic effects of etoposide. The results point to the possibility that combination of etoposide and a modulator might not improve responses to chemotherapy by targeting P-gp or MRP exclusively.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Brügger D,Herbart H,Gekeler V,Seitz G,Liu C,Klingebiel T,Orlikowsky T,Einsele H,Denzlinger C,Bader P,Niethammer D,Beck JFdoi
10.1016/s0145-2126(98)00192-1keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
467-75issue
5eissn
0145-2126issn
1873-5835pii
S0145212698001921journal_volume
23pub_type
杂志文章abstract：:The expression of various proto-oncogenes in primary culture of lymphocytes from peripheral blood of bovine with chronic lymphocytic leukemia (CLL) was studied. Cellular proto-oncogenes encode proteins that propagate growth, differentiation or apoptosis signals from cell membrane to nucleus. The proliferation and diff...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(97)00128-8
更新日期：1998-02-01 00:00:00
abstract：:T-cell leukemias were induced in adult BDF1 mice by a single i.v. injection of methylnitrosourea (MNU). Leukemogenesis was delayed by a single or repeated injections of hydrocortisone (HC) after MNU and also when HC was given one day before MNU. Enhancement of leukemogenesis was seen in experiments with 10 and 14 days...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(85)90143-2
更新日期：1985-01-01 00:00:00
abstract：:Allogeneic hematopoietic stem cell transplantation provides curative therapy for some patients with advanced hematologic malignancies. Disease response after allogeneic transplant is, at least in part, mediated by donor immune cells. In this report we describe a cellular therapy using haploidentical peripheral blood s...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2008.05.015
更新日期：2008-12-01 00:00:00
abstract：:Stromal cells from pediatric myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) associated with MDS (MDS-AML) present high expression of leukemia inhibitor factor (LIF). We demonstrated using mitogen-activated protein kinase (MAPK) inhibitors that in stromal cells from pediatric MDS and MDS-AML, p38MAPK ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.10.012
更新日期：2010-04-01 00:00:00
abstract：:We evaluated the effect of SCF on myeloid differentiation by correlating clonogenic potential (as CFU-GM), bone marrow (BM) plasma SCF levels and CD34/c-kit expression in 57 MDS samples. There was a significant correlation between low SCF levels and 'leukemic' in vitro growth, the number of clusters and the colony/clu...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(98)00175-1
更新日期：1999-03-01 00:00:00
abstract：:In a patient affected with chronic lymphocytic leukemia with lymphocyte surface mu and kappa determinants and vacuolated bone marrow plasma cells, the serum contained polymers of a truncated mu chain and normal-sized kappa chains. These light chains were present as monomers and covalent dimers in studies performed und...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(93)90129-9
更新日期：1993-06-01 00:00:00
abstract：:Ten patients with acute nonlymphocytic leukemia (ANLL) received bromodeoxyuridine (BrdU) at 100 mg/M2 intravenously over 1 h. BrdU is incorporated into the DNA by S-phase cells and was detected by using a monoclonal anti-BrdU antibody in the bone marrow aspirate (BM) and biopsy specimens obtained at the end of the inf...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90160-3
更新日期：1987-01-01 00:00:00
abstract：:A synthetic pentadecapeptide preparation, env 406-420, with an amino acid sequence deduced from the envelope glycoprotein gene of human T cell leukemia virus type I (HTLV I), was used as the antigen in an enzyme immunoassay for immunoglobulin G antibodies, exploring its usefulness for seroepidemiological purposes. The...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(85)90100-6
更新日期：1985-01-01 00:00:00
abstract：:Serial samples of peripheral blood were obtained from 75 children with acute lymphoblastic leukemia in remission. An immunofluorescence assay was used to quantitate TdT-containing (terminal deoxynucleotidyl transferase-containing) cells in the mononuclear leukocyte fraction of these specimens. Nine relapses in 8 patie...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90089-0
更新日期：1987-01-01 00:00:00
abstract：:Limited information is available on the relationship between expression of some additional aberrant phenotypic features and outcome of acute promyelocytic leukemia (APL) patients. Here, we set out to assess the frequency of CD15 and CD56 expression, and their prognostic value in a large series of APL patients. One hun...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.11.008
更新日期：2014-02-01 00:00:00
abstract：:Recently, we identified a novel chromosomal rearrangement in Hodgkin lymphoma (HL), t(4;8)(q27;q24), which targets homeobox gene ZHX2 at the recurrent breakpoint 8q24. This aberration deletes the far upstream region of ZHX2 and results in silenced transcription pinpointing loss of activatory elements. Here, we have lo...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.10.019
更新日期：2012-05-01 00:00:00
abstract：:The direct effect of the feline leukaemia virus (FeLV) on erythroid colony formation in vitro was investigated. Bone marrow mononuclear cells (BMMC) from FeLV-naïve, specific-pathogen-free (SPF), adult cats were inoculated with FeLVs of characterized strains and biologically cloned subgroups and the subsequent develop...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(86)90237-7
更新日期：1986-01-01 00:00:00
abstract：:The PRAME gene encodes an antigen recognized by autologous T lymphocytes and is expressed in trophoblasts, testis and frequently in human solid cancers and acute leukemias, making it a candidate for immunotherapy and for detecting MRD. We demonstrate expression of PRAME by RT-PCR in the peripheral blood or bone marrow...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(02)00217-5
更新日期：2003-05-01 00:00:00
abstract：:Up to 30% of patients with acute myeloid leukemia (AML) and abnormal cytogenetics have persistent cytogenetic abnormalities (pCytAbnl) at morphologic complete remission (mCR). We hypothesized that the prognostic significance of pCytAbnl in patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) i...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2014.11.007
更新日期：2015-01-01 00:00:00
abstract：:An insertion (10;11)(p11;q23q24) was found in bone marrow metaphase cells from two children with acute monocytic leukemia (AMoL-M5b). This rearrangement involves a small chromosomal segment of 11q and may be misinterpreted as a deletion of 11q. Insertion (10;11) may represent a new recurring abnormality involving 11q ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(85)90140-7
更新日期：1985-01-01 00:00:00
abstract：:The methylation inhibitor decitabine (DAC) has great therapeutic value for myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). However, DAC monotherapy is associated with relatively low rates of overall response and complete remission. Previous studies have shown promising results for combination treatmen...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2016.02.002
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVES:Follicular lymphoma (FL) is generally considered an indolent disorder but a significant subset of patients shows a worse outcome. Aim of this study was to validate the FLIPI score in an independent series of follicular lymphoma patients and to correlate prognostic categories with the period of diagnosis and ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.07.006
更新日期：2006-03-01 00:00:00
abstract：:The aim of this study is to detect the biologic and/or prognostic significance of survivin (S) and effector protease receptor 1: EPR-1 (E) expression in acute leukemias (34 ALL and 40 AML) by using RT-PCR. S and E expressions were found in 83.8 and 20.3% of the cases, respectively. S was detected in 90%, 76.5% and E w...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.01.005
更新日期：2007-11-01 00:00:00
abstract：:Ml cells were labeled in vitro with [3H]thymidine and injected into the peritoneal cavity of syngeneic mice. After several days the peritoneal cells were harvested and [3H]labeled cells were determined by autoradiography. The inoculated isotope-labeled Ml cells differentiated in the peritoneal cavity and lipopolysacch...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(82)90050-9
更新日期：1982-01-01 00:00:00
abstract：:The human lymphoblastoid leukemic cell line (CCRF-CEM) was induced to differentiate with phorbol 12-myristate 13-acetate (PMA). During differentiation, assessed by monitoring the cluster of differentiation (CD) profile, the prosome (proteasomes, multi-catalytic proteinase) distribution and composition were studied by ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(97)00091-x
更新日期：1997-11-01 00:00:00
abstract：:Because of the teratogenicity data in rats, it is recommended that women treated with imatinib should be aware of the potential teratogenicity of imatinib and effective contraception should be used during imatinib therapy to prevent pregnancy. We describe successful pregnancy and delivery, without any congenital anoma...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.01.009
更新日期：2005-08-01 00:00:00
abstract：:To further understand the role of XIAP in acute myeloid leukemia (AML), we suppressed XIAP expression by antisense oligonucleotides and determined the effect on gene expression profiles and biological pathways. XIAP inhibition upregulated expression of proteasome genes in a manner similar to the proteasome inhibitor b...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.04.018
更新日期：2013-08-01 00:00:00
abstract：:We have previously shown that forced expression of the transcription factor Scl in WEHI-3B D(+) cells prevents ATRA-induced cell differentiation. We now find that the overexpression of Rbtn2 also interferes with induction of differentiation by ATRA. Addition of LiCl to ATRA treatment restored the capacity of both Scl-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2003.08.011
更新日期：2004-04-01 00:00:00
abstract：:We investigated Syk as a potential marker of CML progression. We observed a significant over-expression of Syk mRNA and constitutive phosphorylation of Syk Y348 in blast cells from six AP or BP-CML, but not in 15 CML in chronic phase. We could follow in vivo the recurrence of pSyk(348) throughout blast cell escape, de...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.11.014
更新日期：2015-03-01 00:00:00
abstract：:The functional behavior of hematopoietic stem cell (HSC) and progenitors in chronic myelomonocytic leukemia (CMML) and on disease progression is little known. We performed cell proliferation, apoptosis, hematopoietic colony forming/replating and differentiation potential studies in the purified subpopulations of Lin(-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.02.011
更新日期：2008-09-01 00:00:00
abstract：:Although clinical experience and in vitro data provide evidence of an anti-leukemic activity of T cells, there are few examples of recognition of leukemic cells by tumor-specific T cells in vitro. Tumor antigens encoded by the MAGE genes are useful tools to study this recognition. We tested the sensitivity to recognit...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(00)00053-9
更新日期：2000-10-01 00:00:00
abstract：:Leukemic blasts of two patients with acute leukemia exhibited similar characteristics. They were heterogeneous in size with a diameter of 14-30 microns in smears and unclassifiable by morphological, cytochemical, immunophenotypic and ultrastructural examinations. Cytogenetic examinations of both revealed a near-tetrap...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90087-6
更新日期：1994-07-01 00:00:00
abstract：:Stem cell factor (SCF) is a growth factor that promotes the survival, proliferation, and differentiation of hematopoietic cells. SCF and its receptor, Kit, are normally present in both cell surface and soluble forms. Both forms of Kit can bind SCF. However, the function of soluble Kit is unknown. In order to determine...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(00)00122-3
更新日期：2001-05-01 00:00:00
abstract：:In 2008 the first evidence-based Canadian consensus guideline addressing the diagnosis, monitoring and management of transfusional iron overload in patients with myelodysplastic syndromes (MDS) was published. The Canadian Consortium on MDS, comprised of hematologists from across Canada with a clinical and academic int...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2018.09.005
更新日期：2018-11-01 00:00:00
abstract：:We compared the growth-inhibitory effects and inhibition profile of the SRC family kinases (SFKs) of imatinib, dasatinib, nilotinib and INNO-406. Dasatinib exhibited the strongest potency against BCR-ABL with little selectivity over SFKs. Nilotinib exhibited a weaker affinity than the other inhibitors, but was highly ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.11.008
更新日期：2008-06-01 00:00:00
