Abstract:
:In acute lymphoblastic leukemia (ALL) patients, automated fluorescence fragment analysis (ALF) has been reported to improve the monoclonality detection rate of immunoglobulin heavy chain genes (IgH) polymerase chain reaction (PCR) analysis. This study performed complementary determining region (CDR) I and III PCR on samples from 135 patients with B-cell neoplasias and 25 healthy controls. The value of ALF was investigated in comparison to the widely used ethidium bromide (ETB)-stained agarose gels (AGGE). ETB-stained AGGE detected monoclonal CDR III PCR products in 53/72 ALL, in 22/34 non-Hodgkin's lymphoma (NHL), 13/22 multiple myeloma (MM), and 2/7 monoclonal gammopathies (MGUS). ALF identified monoclonal CDR III amplificates in 55/72 ALL, 23/34 B-NHL, 14/22 MM, and 2/7 MGUS. AGGE achieved clonal CDR I PCR results in 30/64 samples, while ALF detected 34 clonal CDR I product patterns. Taking together, ETB-stained AGGE revealed monoclonality in 120/199 PCR products versus 129/199 by ALF. Compared with AGGE and ETB-staining, ALF offers a slightly increased sensitivity and can be recommended for the evaluation of difficult samples.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Gleissner B,Maurer J,Sindram A,Reinhard R,Thiel Edoi
10.1016/s0145-2126(01)00025-xkeywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
769-74issue
9eissn
0145-2126issn
1873-5835pii
S0145-2126(01)00025-Xjournal_volume
25pub_type
杂志文章abstract::Early T-cell precursor-ALL (ETP-ALL) is a subtype of T-ALL with a poor prognosis in children. We analyzed ETP-ALL compared to conventional T-ALL/LBL in both adults and children to determine any differences in clinical outcomes, based on the following parameters: induction failure, relapse, and survival. Patients with ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.06.010
更新日期:2013-09-01 00:00:00
abstract::Septins are proteins associated with crucial steps in cell division and cellular integrity. In humans, 14 septin genes have been identified, of which five (SEPT2, SEPT5, SEPT6, SEPT9, and SEPT11) are known to participate in reciprocal translocations with the MLL gene in myeloid neoplasias. We have recently shown a sig...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.08.018
更新日期:2010-05-01 00:00:00
abstract::Information about the expression of some myelomonocytic markers in lymphocytes of patients with B-CLL is scarce. We studied the CD13, CD14, CD11c and CD68 surface antigens in 42 controls and in 38 patients with B-CLL to detect their possible reactivity. Eighty-nine percent of B-CLL expressed very strongly the CD14 ant...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(92)90177-9
更新日期:1992-01-01 00:00:00
abstract::A case of acute monocytic leukemia (AMoL) with t(5;11)(q31;q23) and a case of acute myelomonocytic leukemia (AMMoL) with t(5;11)(q35;q13.1) are reported. The translocation between the long arm of chromosome 11q and that of chromosome 5q with leukemia have been rarely reported. Though breakpoint of both cases were subt...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(99)00033-8
更新日期:1999-07-01 00:00:00
abstract::Discovering genetic predictors of childhood acute lymphoblastic leukemia (ALL) necessitates the evaluation of novel factors including maternal genetic effects, which are a proxy for the intrauterine environment, and robust epidemiologic study designs. Therefore, we evaluated five maternal and offspring xenobiotic meta...
journal_title:Leukemia research
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.leukres.2013.01.020
更新日期:2013-05-01 00:00:00
abstract::We investigated the transcript levels of genes STAT1, STAT3, STAT5A and STAT5B in the diagnostic samples of childhood ALL patients and compared them to those of healthy controls in order to characterize STAT gene expression in childhood ALL. As compared to controls, ALL patients exhibit markedly decreased transcript l...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2015.09.004
更新日期:2015-09-11 00:00:00
abstract::We report a unique case of de novo acute promyelocytic leukemia (APL) with cryptic 15;17 rearrangements. Cytogenetically, structural rearrangements of the 6p23 region has been reported mainly in secondary leukemia. This patient had a karyotype of 46, XY, del(6)(p23) and no additional chromosomal abnormalities. Molecul...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(99)00139-3
更新日期:2000-01-01 00:00:00
abstract::We investigated the expression of fos oncogene proteins in lymphoproliferative disorders, using a monoclonal antibody (FO-120) that was prepared against a synthetic oligopeptide of fos protein (amino acid sequence from 127 to 152). Although peripheral blood leukocytes were rarely positive for FO-120, they were transie...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90010-6
更新日期:1989-01-01 00:00:00
abstract::The capacity of human thymocytes and of differentiated lymphocytes circulating in peripheral blood to perform unscheduled DNA synthesis (a measure of nucleotide excision repair) after UV irradiation was measured by radioautographic analysis. Only 4% of immature T lymphocytes, but 68% of circulating lymphocytes exhibit...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(85)90006-2
更新日期:1985-01-01 00:00:00
abstract::Imatinib is a highly effective drug for the treatment of chronic myeloid leukemia (CML) that targets the BCR-ABL kinase. However, a number of patients have CML that is resistant to Imatinib treatment. In this report, we developed AGM130 as a potential therapeutic drug for Imatinib-resistant CML treatment. The AGM130 c...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.12.017
更新日期:2013-04-01 00:00:00
abstract::Cases of leukemia associated with Turner syndrome (TS) are rare. Here we report three TS patients with leukemia including one case of T-large granular lymphocyte leukemia (T-LGL), one rare case of coexistence of chronic lymphocytic leukemia (CLL) and idiopathic myelofibrosis (IMF) and one case of a patient with AML-M2...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2007.06.004
更新日期:2008-03-01 00:00:00
abstract::The peripheral blood lymphocyte to monocyte ratio (LMR) at diagnosis has been used to predict survival in diffuse large B-cell lymphoma (DLBCL) patients, but its prognostic significance with respect to different cell-of-origin (COO) subtypes remains unknown. We retrospectively analyzed 168 de novo DLBCL patients in th...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2014.03.013
更新日期:2014-06-01 00:00:00
abstract::We evaluated the effect of SCF on myeloid differentiation by correlating clonogenic potential (as CFU-GM), bone marrow (BM) plasma SCF levels and CD34/c-kit expression in 57 MDS samples. There was a significant correlation between low SCF levels and 'leukemic' in vitro growth, the number of clusters and the colony/clu...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(98)00175-1
更新日期:1999-03-01 00:00:00
abstract::GNAS1 gene is located at the long arm of chromosome 20 (q13.32). GNAS1 gene deletion has never been investigated in MDS. A GNAS1 activating mutation (R201) was recently found in MDS. We applied FISH and DHPLC plus sequencing to investigate GNAS1 gene in MDS cases with and without del(20q) at karyotype. ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2014.03.017
更新日期:2014-07-01 00:00:00
abstract::The Philadelphia (Ph1) chromosome is a specific structural abnormality in which the abl oncogene is activated due to the formation of the novel chimeric gene, bcr/abl. To investigate the clinicopathological role of bcr/abl in Ph1-positive chronic myelogenous leukaemia (CML), we studied the clonal origin of haematopoie...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(92)90139-x
更新日期:1992-01-01 00:00:00
abstract::This study was conducted to determine the frequency of the most common fusion genes in Mexican pediatric patients with acute lymphoblastic leukemia (ALL). Molecular analysis using RT-PCR was carried out in 53-blood samples: 52 patients with de novo ALL and one with relapsed ALL. The ETV6-RUNX1 fusion was found in 7 ca...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.03.021
更新日期:2008-10-01 00:00:00
abstract::We investigated the effects of two sex steroids (17beta estradiol and testosterone) on five human leukemia cell lines. We observed a statistically significant inhibition of proliferation, dose and time dependent, of the human monoblastic leukemia cell line U937. This inhibition was associated with a dose dependent dec...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(98)00101-5
更新日期:1998-11-01 00:00:00
abstract::Leukemic cells from 85 children with newly diagnosed precursor B-lineage ALL were tested for in vitro drug sensitivity to a panel of anti-cancer drugs. Minimal residual disease (MRD) was measured by RQ-PCR. There was a significant correlation between MRD day 29 and in vitro sensitivity to prednisolone (p<0.001) and do...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.06.012
更新日期:2009-01-01 00:00:00
abstract::This study investigated radioresistance mechanisms in the doxorubicin-resistant acute myelogenous leukemia (AML)-2/DX100. AML-2/DX100 also showed resistance to radiation. AML-2/DX100 characterized by down-regulated catalase expression was supersensitive to exogenous hydrogen peroxide whereas they increased defense mec...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.11.006
更新日期:2007-09-01 00:00:00
abstract::Chromosomal changes are necessary in determining the classification, prognosis and using the appropriate therapeutic regimen in acute leukemia. Isochromosomes are uncommon chromosome aberations in childhood acute lymphoblastic leukemia (ALL) and the effect of i(9q) is not well established. We present an 8-year-old mal...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.02.018
更新日期:2006-11-01 00:00:00
abstract::Acute myeloid leukemia (AML) is a heterogeneous disease. Until recently, treatment for patients with AML was limited to induction chemotherapy with cytarabine and anthracycline or hypomethylating agents, and, in some instances, allogeneic hematopoietic stem cell transplant. With the recent approval of new therapies-i....
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2018.09.001
更新日期:2018-10-01 00:00:00
abstract::Despite the high effectiveness of various P-glycoprotein (P-gp) modulating substances in vitro their clinical value e.g. for combination treatment of acute myelogenous leukemias (AML) remains still unclear. This might be explainable by recent findings that other factors than P-gp (e.g. the multidrug resistance associa...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(98)00192-1
更新日期:1999-05-01 00:00:00
abstract::Stromal cells from pediatric myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) associated with MDS (MDS-AML) present high expression of leukemia inhibitor factor (LIF). We demonstrated using mitogen-activated protein kinase (MAPK) inhibitors that in stromal cells from pediatric MDS and MDS-AML, p38MAPK ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.10.012
更新日期:2010-04-01 00:00:00
abstract::A prospective case-control study of the aetiologic factors involved in the production of lymphoid malignancies has been conducted within a defined geographical area covering six health districts in the Yorkshire Region. Among the aspects investigated were past medical events, occupations and certain social factors. A ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(84)90016-x
更新日期:1984-01-01 00:00:00
abstract::The effect of mesna on intracellular accumulation of cytosine arabinoside (Ara-C) in murine normal and neoplastic lymphocytes was studied. Simultaneous exposure of cells to mesna at concentrations ranging from 0.25 to 1.0 mM and 3H-Ara-C (40.0 nM) resulted in a strong inhibition of Ara-C uptake in normal lymphocytes. ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(93)90117-4
更新日期:1993-09-01 00:00:00
abstract::A panel of human leukemia cell lines from various lineages (T-cell, pre B- and Non-T/Non-B cell, myelomonocytic and erythroleukemia cell lines) were utilised as model systems of the distant effects of differentiation-inducing activity (DIA) produced by the T-leukemia cell line HUT-102. DIA inhibited cell proliferation...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90108-1
更新日期:1987-01-01 00:00:00
abstract::To study the role of SDF-1/CXCR4 axis in MDS, the expression of SDF-1 and CXCR4, VEGF, MVD and apoptosis were measured in MDS. The results showed that the expression of SDF-1 of the low-grade MDS is higher than that of the high-grade MDS and the control. The high-grade MDS had a significantly higher CXCR4 expression o...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2011.06.017
更新日期:2012-03-01 00:00:00
abstract::A variety of genetic and epigenetic abnormalities were characterized over the last years in Hodgkin and Reed-Sternberg (H-RS) cells of classic Hodgkin Lymphoma (cHL). It was speculated that simultaneous inhibition of multiple signalling pathways might be a promising strategy to target this tumor entity. In the present...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.12.013
更新日期:2009-07-01 00:00:00
abstract::Myelodysplastic syndromes (MDS) and chronic myelomonocytic leukemia (CMML) may be underreported in cancer registries such as the Netherlands Cancer Registry (NCR). Analysis of Dutch medical claims can complement NCR data on MDS and CMML. We analyzed data on 3681 MDS patients and 235 CMML patients aged ≥18 years with i...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2014.11.025
更新日期:2015-02-01 00:00:00
abstract::Delocalized lipophilic cation dequalinium (DQA) selectively accumulates in mitochondria and displays anticancer activity in different malignancies. Our previous studies indicate a DQA-induced cytotoxicity in human acute promyelocytic leukemia NB4 cells by early disturbance in mitochondrial function and oxidative stres...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2014.01.009
更新日期:2014-07-01 00:00:00