Abstract:
:Typhoid fever is a persistent infection caused by host-adapted Salmonella strains adept at circumventing immune-mediated host defences. Given the importance of T cells in protection, the culling of activated CD4+ T cells after primary infection has been proposed as a potential immune evasion strategy used by this pathogen. We demonstrate that the purging of activated antigen-specific CD4+ T cells after virulent Salmonella infection requires SPI-2 encoded virulence determinants, and is not restricted only to cells with specificity to Salmonella-expressed antigens, but extends to CD4+ T cells primed to expand by co-infection with recombinant Listeria monocytogenes. Unexpectedly, however, the loss of activated CD4+ T cells during Salmonella infection demonstrated using a monoclonal population of adoptively transferred CD4+ T cells was not reproduced among the endogenous repertoire of antigen-specific CD4+ T cells identified with MHC class II tetramer. Analysis of T-cell receptor variable segment usage revealed the selective loss and reciprocal enrichment of defined CD4+ T-cell subsets after Salmonella co-infection that is associated with the purging of antigen-specific cells with the highest intensity of tetramer staining. Hence, virulent Salmonella triggers the selective culling of high avidity activated CD4+ T-cell subsets, which re-shapes the repertoire of antigen-specific T cells that persist later after infection.
journal_name
Immunologyjournal_title
Immunologyauthors
Ertelt JM,Johanns TM,Mysz MA,Nanton MR,Rowe JH,Aguilera MN,Way SSdoi
10.1111/j.1365-2567.2011.03510.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
487-97issue
4eissn
0019-2805issn
1365-2567journal_volume
134pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Thoracic duct lymphocytes (TDL) from (AS2 x AS)F1 rats previously injected with human serum albumin (HSA) were transferred to 900 r irradiated syngeneic recipients which were challenged with various doses of soluble HSA (s-HSA). TDL from partially tolerant rats, which were deficient in high avidity B cells, produced a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-10-01 00:00:00
abstract::A technique is reported here for the quantitative extraction of live cells from the lung interstitium; it involves the incubation of slices of perfused lung in a mixture containing optimal concentrations of collagenase, DNAse, and fetal calf serum, followed by the simultaneous recovery and fractionation of cells relea...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Cells of the female reproductive tract (FRT) can present antigen to naive and memory T cells. However, the effects of oestrogen, known to modulate immune responses, on antigen presentation in the FRT remain undefined. In the present study, DO11.10 T-cell antigen receptor transgenic mice specific for the class II MHC-r...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03512.x
更新日期:2012-01-01 00:00:00
abstract::Anti-Gal is the most abundant natural antibody in humans, constituting ~ 1% of immunoglobulins. Anti-Gal is naturally produced also in apes and Old World monkeys. The ligand of anti-Gal is a carbohydrate antigen called the 'α-gal epitope' with the structure Galα1-3Galβ1-4GlcNAc-R. The α-gal epitope is present as a maj...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12110
更新日期:2013-09-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium (S. typhimurium) causes a localized enteric infection and its elimination is dependent on a T helper type 1 immune response. However, the mechanism of the protective immune response against the pathogen in gut-associated lymphoid tissue (GALT) at an early stage of the infection ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03310.x
更新日期:2010-11-01 00:00:00
abstract::A plasmid that contained the cytomegalovirus (CMV)-promoter-driven lacZ reporter gene (pCMV-lacZ) remained in the epaxial muscle of five of eight goldfish as covalently closed circles, the most functional form of plasmid, for at least 70 days at 22 degrees. It was not present in the gills or elsewhere by polymerase ch...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00688.x
更新日期:1999-02-01 00:00:00
abstract::Expression of the lymph node homing and CC-chemokine receptor 7 (CCR7), with L-selectin (CD62L), has been shown to divide human memory T cells into two functionally distinct subsets. We generated a polyclonal antibody against murine CCR7 and used this antibody to study CCR7 expression on murine T-cell subsets. Using f...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01727.x
更新日期:2003-10-01 00:00:00
abstract::Mouse lymph node cells cultured with Concanavalin A (Con A) in serum-free medium containing 59Fe-transferrin took up 59Fe more rapidly than cells cultured without Con-A. Uptake of iron commenced rapidly and preceded the onset of DNA synthesis in stimulated cells. Total uptake of transferrin during culture was much low...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::The non-obese diabetic (NOD) mouse is an animal model for insulin-dependent diabetes that has many similarities to the human disease. NOD mice transgenic for the Ea gene, allowing expression of the E molecule, are protected from diabetes and rarely develop insulitis. An Ea transgene mutated in the promoter region, (De...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1998.00580.x
更新日期:1998-09-01 00:00:00
abstract::The CTLL-2 bioassay is used frequently to determine interleukin-2 (IL-2) concentrations in experimental samples, including samples that contain reagents which affect the CD28-B7 interaction. We therefore evaluated whether the CD28-B7 pathway plays a role in the growth of CTLL-2 cells. Flow cytometry demonstrated that ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.461532.x
更新日期:1996-02-01 00:00:00
abstract::Nod-like receptors are a family of innate immune receptors that link cytosolic sensing of microbial and danger stimuli to the activation of immune responses. Two Nod-like receptor family members, Nod1 and Nod2, recognize bacterial peptidoglycan and activate immune responses via nuclear factor-κB (NF-κB) and mitogen-ac...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12307
更新日期:2014-10-01 00:00:00
abstract::Animal models of rheumatoid arthritis (RA) have provided substantial insights into basic pathogenic mechanisms of chronic inflammatory arthritis and autoimmune disease in general. Of the variety of models reported, collagen-induced arthritis (CIA) has been the most characterized in terms of both its pathogenesis and i...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2567.2001.01267.x
更新日期:2001-08-01 00:00:00
abstract::Stimulated T lymphocytes and certain T-cell hybridomas secrete molecules capable of inducing B-lymphocyte proliferation and differentiation. It has been shown recently that one such B-cell stimulatory factor is associated with chondroitin sulphate proteoglycan (CSPG) and was designated T-cell proteoglycan fraction, or...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::The influence of the thymus and the possible requirement for surface immunoglobulin expression for the development of K and NK activity were assessed in rats. This species was chosen in preference to mice as they show good levels of K-cell activity. Studies were carried out in athymic (rnu/rnu) rats some of which were...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::Large doses of hydrocortisone, cyclophosphamide, and methotrexate injected subcutaneously, and whole-body irradiation (500 rads) caused a reduction in the number of peritoneal cells (PE cells) obtained after intraperitoneal injection of the treated mice with proteose-peptone. The same dose of cyclophosphamide and irra...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::The anti-C6 monoclonal antibody WU 6-4 was shown to be unequivocally native restricted since it neither binds to the terminal complement complex (TCC) nor to C5b6. In addition, it was shown to inhibit TCC formation by interfering with C5b6 generation. Using the pUEX expression system and C6 cDNA the WU 6-4 epitope was...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-06-01 00:00:00
abstract::The response of peripheral blood mononuclear cells to pokeweed mitogen (PWM) stimulation was investigated with combined light (LM) and scanning electron microscopy (SEM). The DNA content of the nucleus of a cell (measured by fluorescence) was compared directly with the diameter and morphological features (by SEM). It ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::Examples suggesting that all or part of the V(H) segment of a rearranged V(H)DJ(H) may be replaced by all or part of another V(H) have been appearing since the 1980s. Evidence has been presented of two rather different types of replacement. One of these has gained acceptance and has now been clearly demonstrated to oc...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2004.02084.x
更新日期:2005-02-01 00:00:00
abstract::Bacillus Calmette-Guérin (BCG) inoculation has been shown to inhibit certain immune functions. To determine whether this inhibition adversely affects host defences against infection, the effect of BCG on Listeria infection in mice was investigated. Mice were injected intravenously (i.v.) with Listeria monocytogenes an...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::The adult cockroach generates an adaptive humoral immune response exhibiting specificity and immunological memory when immunized with soluble proteins. In contrast, the response induced by bacteria in holometabolous insects is non-specific and short term, generally losing activity after 72 hr. We have found that the r...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
abstract::Guinea-pig erythrocytes that had been exposed to influenza A virus or Vibrio cholerae neuraminidase activated the classical complement pathway in autologous serum. Because all viral particles were eluted from the treated cells, activation was not dependent on anti-viral antibodies or on the particles themselves. After...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract::Thymocytes expressing self-reactive T-cell receptors (TCR) are eliminated in the thymus through a TCR-mediated signal. This cell death signal (negative selection) generates nuclear morphological change and DNA fragmentation in thymocytes. However, the pathway leading to DNA fragmentation of thymocytes following TCR en...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01347.x
更新日期:2002-04-01 00:00:00
abstract::Norovirus (NoV) -derived virus-like particles (VLPs) resemble empty shells of the virus and NoV P-particles contain only protruding domains of the NoV capsid. Both NoV-derived subviral particles show similar functionality and antigenicity in vitro and are considered to be potential vaccine candidates against NoV gastr...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03516.x
更新日期:2012-01-01 00:00:00
abstract::Vaccination strategies that can block or limit heterosexual human immunodeficiency virus (HIV) transmissions to local and systemic tissues are the goal of much research effort. Herein, in a mouse model, we aimed to determine whether the enhancement of antibody responses through mucosal and systemic immunizations, prev...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2007.02702.x
更新日期:2008-03-01 00:00:00
abstract::We explored the mechanisms of class B CpG-oligodeoxynucleotide-induced antitumour effects against weakly immunogenic tumours. Treatment with CpG-oligodeoxynucleotide 1826 (CpG) induced similar antitumour effects in B16 melanoma-bearing immunocompetent C57BL/6 mice and T-cell-deficient severe combined immunodeficient (...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02517.x
更新日期:2007-03-01 00:00:00
abstract::Lymphocytes are heterogeneous with respect to their life-span. Typical B cells, bearing on their membranes immunoglobulin receptors, easily detectable by immunofluorescence, belong mainly to the long-lived population: this can be observed using combined autoradiography and immunofluorescence. However, when primed mice...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-05-01 00:00:00
abstract::Enriched CD4+CD8-/CD4-CD8-, CD4-CD8+/CD4-CD8- and CD4-CD8- cell suspensions were prepared from normal peripheral blood by selective immunomagnetic depletion of monoclonal antibody-defined lymphocyte populations. Subsequent examination of these modified cell fractions by two-colour flow cytometry provided a means of de...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::Immunization of mice with cognate cancer-derived heat-shock protein (hsp) preparations leads to protection from cancer growth. As hsp used for vaccination or therapy are derived from autologous cancers, questions of pathological autoimmunity are of immense significance for the ongoing translation of this approach to t...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00127.x
更新日期:2000-11-01 00:00:00
abstract::Using cloned human helper T lymphocytes reactive with a 24 amino acid peptide (p20) of the carboxyl terminal of the HA-1 molecule of influenza haemagglutinin we have investigated the influence of antigen structure on the activation and in the induction of antigen specific unresponsiveness of T cells. For this analysis...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::Murine embryonic stem (ES) cell-derived haematopoietic progenitor cells (HPCs) engraft and populate lymphoid organs. In vivo, HPCs engraft across MHC barriers protecting donor-type allografts from rejection. However, the underlying phenomenon remains elusive. Here, we sought to determine the mechanism by which ES cell...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12268
更新日期:2014-07-01 00:00:00