Structural role of the terminal disulfide bond in the sweetness of brazzein.

Abstract:

:Brazzein, a 54 residue sweet-tasting protein, is thought to participate in a multipoint binding interaction with the sweet taste receptor. Proposed sites for interaction with the receptor include 2 surface loops and the disulfide bond that connects the N- and C-termini. However, the importance of each site is not well understood. To characterize the structural role of the termini in the sweetness of brazzein, the position of the disulfide bond connecting the N- and C-termini was shifted by substituting K3-C4-K5 with C3-K4-R5. The apparent affinity and V(max) of the C3-K4-R5-brazzein (CKR-brazzein) variant were only modestly decreased compared with the wild-type (WT) brazzein. We determined a high-resolution structure of CKR-brazzein by nuclear magnetic resonance spectroscopy (backbone root mean square deviation of 0.39 Å). Comparing the structure of CKR-brazzein with that of WT-brazzein revealed that the terminal β-strands of the variant display extended β-structure and increased dynamics relative to WT-brazzein. These results support previous mutagenesis studies and further suggest that, whereas interactions involving the termini are necessary for full function of brazzein, the termini do not constitute the primary site of interaction between brazzein and the sweet taste receptor.

journal_name

Chem Senses

journal_title

Chemical senses

authors

Dittli SM,Rao H,Tonelli M,Quijada J,Markley JL,Max M,Assadi-Porter F,Maillet E

doi

10.1093/chemse/bjr057

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

821-30

issue

9

eissn

0379-864X

issn

1464-3553

pii

bjr057

journal_volume

36

pub_type

杂志文章
  • Study of the olfactory epithelium in the developing sturgeon. Characterization of the crypt cells.

    abstract::In acipenserids, crypt cells (CCs) have only been observed in juvenile specimens, and it has not been clarified whether they differentiate along with olfactory receptor neurons (ORNs) during the lecithotrophic stage or during later development stages. Furthermore, no detailed optical microscopy (OM) or electron micros...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjp091

    authors: Camacho S,Ostos-Garrido MV,Domezain A,Carmona R

    更新日期:2010-02-01 00:00:00

  • Preference for sucralose predicts behavioral responses to sweet and bittersweet tastants.

    abstract::Rats can be classified as either sucralose avoiders (SA) or sucralose preferrers (SP) based on their behavioral responses in 2-bottle preference, 1-bottle intake, and brief-access licking tests. The present study demonstrates that this robust phenotypic variation in the preference for sucralose predicts acceptance of ...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjr126

    authors: Loney GC,Torregrossa AM,Carballo C,Eckel LA

    更新日期:2012-06-01 00:00:00

  • Chemosensory cross-modal stroop effects: congruent odors facilitate taste identification.

    abstract::In order to explore the cross-modal cognitive associations between smell and taste, a chemosensory analogue of the Stroop task (Stroop 1935) was developed. Fourteen participants were presented with an odorant and a tastant and asked to identify the tastant as "sweet" or "sour" by pressing 1 of 2 buttons as quickly as ...

    journal_title:Chemical senses

    pub_type: 杂志文章,随机对照试验

    doi:10.1093/chemse/bjm001

    authors: White TL,Prescott J

    更新日期:2007-05-01 00:00:00

  • Odor-evoked autobiographical memories: age and gender differences along the life span.

    abstract::Odors are powerful in bringing back old and vivid memories bearing emotional content. This inherent hedonic property of olfactory stimuli makes this sensory modality particularly suitable for studying autobiographical memory. In the present work, adolescents (first experiment), young adults (second experiment), and el...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjr089

    authors: Zucco GM,Aiello L,Turuani L,Köster E

    更新日期:2012-02-01 00:00:00

  • Olfactory cocainization is not an effective long-term treatment for phantosmia.

    abstract::Phantosmia, the perception of an odor when there are no odorants in the environment, can be a very debilitating symptom. In the 1960s, Zilstorff reported olfactory distortions could be treated by the topical application of a cocaine solution to the olfactory epithelium. In evaluating this treatment, we observed no lon...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjt047

    authors: Leopold DA,Hornung DE

    更新日期:2013-11-01 00:00:00

  • Nestmate recognition cues in the honey bee: differential importance of cuticular alkanes and alkenes.

    abstract::In social insects, recognition of nestmates from aliens is based on olfactory cues, and many studies have demonstrated that such cues are contained within the lipid layer covering the insect cuticle. These lipids are usually a complex mixture of tens of compounds in which aliphatic hydrocarbons are generally the major...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bji040

    authors: Dani FR,Jones GR,Corsi S,Beard R,Pradella D,Turillazzi S

    更新日期:2005-07-01 00:00:00

  • A sexually dimorphic group of atypical glomeruli in the mouse olfactory bulb.

    abstract::Atypical glomeruli (AtG) are clearly distinguishable from typical ones because of their strong cholinergic innervation. AtG are located in defined positions in the caudal half of the main olfactory bulb of rodents. The AtG partially overlap with other specialized olfactory subsystems, such as the modified glomerular c...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/26.1.7

    authors: Weruaga E,Briñón JG,Porteros A,Arévalo R,Aijón J,Alonso JR

    更新日期:2001-01-01 00:00:00

  • The distribution of sugar chains on the vomeronasal epithelium observed with an atomic force microscope.

    abstract::The distribution of sugar chains on tissue sections of the rat vomeronasal epithelium, and the adhesive force between the sugar and its specific lectin were examined with an atomic force microscope (AFM). AFM tips were modified with a lectin, Vicia villosa agglutinin, which recognizes terminal N-acetyl-D-galactosamine...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/24.1.1

    authors: Osada T,Takezawa S,Itoh A,Arakawa H,Ichikawa M,Ikai A

    更新日期:1999-02-01 00:00:00

  • Methyl salicylate, identified as primary odorant of a specific receptor neuron type, inhibits oviposition by the moth Mamestra brassicae L. (Lepidoptera, noctuidae).

    abstract::The cabbage moth, Mamestra brassicae L. (Lepidoptera, Noctuidae), is a polyphagous species that is often choosing plants of Brassica as hosts for oviposition. In the search for biologically relevant odorants used by these moths, gas chromatography linked to electrophysiological recordings from single receptor neurons ...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjm061

    authors: Ulland S,Ian E,Mozuraitis R,Borg-Karlson AK,Meadow R,Mustaparta H

    更新日期:2008-01-01 00:00:00

  • The influence of sodium salts on binary mixtures of bitter-tasting compounds.

    abstract::In order to study potential mixture interactions among bitter compounds, selected sodium salts were added to five compounds presented either alone or as binary bitter-compound mixtures. Each compound was tested at a concentration that elicited 'weak' perceived bitterness. The bitter compounds were mixed at these conce...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjh045

    authors: Keast RS,Canty TM,Breslin PA

    更新日期:2004-06-01 00:00:00

  • Arecoline Alters Taste Bud Cell Morphology, Reduces Body Weight, and Induces Behavioral Preference Changes in Gustatory Discrimination in C57BL/6 Mice.

    abstract::Arecoline, a major alkaloid in areca nuts, is involved in the pathogenesis of oral diseases. Mammalian taste buds are the structural unit for detecting taste stimuli in the oral cavity. The effects of arecoline on taste bud morphology are poorly understood. Arecoline was injected intraperitoneally (IP) into C57BL/6 mi...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjv059

    authors: Peng WH,Chau YP,Lu KS,Kung HN

    更新日期:2016-01-01 00:00:00

  • A flux capacitor for moth pheromones.

    abstract::In this issue of Chemical Senses, Baker et al. propose a provocative and intriguing explanation for a commonly observed phenomenon in moth chemocommunication. Sex pheromones in moths typically consist of mixtures of long-chain unsaturated compounds in specific ratios. These ratios are correspondingly detected by male ...

    journal_title:Chemical senses

    pub_type: 评论,杂志文章

    doi:10.1093/chemse/bjs007

    authors: Olsson SB,Hansson BS

    更新日期:2012-05-01 00:00:00

  • Histochemical identification of carbohydrate moieties in the accessory olfactory bulb of the mouse using a panel of lectins.

    abstract::Lectin binding patterns in the olfactory bulb of the mouse were investigated using 12 biotinylated lectins. Three, with specificities for galactose, N-acetylgalactosamine and L-fucose, stained only the nervous and glomerular layers of the accessory olfactory bulb; four, with specificities for galactose or N-acetylgluc...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/26.6.645

    authors: Salazar I,Sánchez Quinteiro P,Lombardero M,Cifuentes JM

    更新日期:2001-07-01 00:00:00

  • Experience Informs Consummatory Choices for Congruent and Incongruent Odor-Taste Mixtures in Rats.

    abstract::Experience is an essential factor informing food choice. Eating food generates enduring odor-taste associations that link an odor with a taste's quality and hedonic value (pleasantness/unpleasantness) and creates the perception of a congruent odor-taste combination. Previous human psychophysical experiments demonstrat...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjaa025

    authors: McQueen KA,Fredericksen KE,Samuelsen CL

    更新日期:2020-05-29 00:00:00

  • Temperature Affects Human Sweet Taste via At Least Two Mechanisms.

    abstract::The reported effects of temperature on sweet taste in humans have generally been small and inconsistent. Here, we describe 3 experiments that follow up a recent finding that cooling from 37 to 21 °C does not reduce the initial sweetness of sucrose but increases sweet taste adaptation. In experiment 1, subjects rated t...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjv021

    authors: Green BG,Nachtigal D

    更新日期:2015-07-01 00:00:00

  • The influence of taste on swallowing apnea, oral preparation time, and duration and amplitude of submental muscle contraction.

    abstract::Prior research has documented a modulating effect of taste on swallowing. We hypothesized that presentation of tastant stimuli would be a significant variable in swallowing-respiratory coordination, duration of oral bolus preparation, and submental muscle contraction. Twenty-three healthy females were presented with 1...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjl037

    authors: Leow LP,Huckabee ML,Sharma S,Tooley TP

    更新日期:2007-02-01 00:00:00

  • Expression patterns of odorant receptors and response properties of olfactory sensory neurons in aged mice.

    abstract::The sense of smell deteriorates in normal aging, but the underling mechanisms are still elusive. Here we investigated age-related alterations in expression patterns of odorant receptor (OR) genes and functional properties of olfactory sensory neurons (OSNs)-2 critical factors that define the odor detection threshold i...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjp056

    authors: Lee AC,Tian H,Grosmaitre X,Ma M

    更新日期:2009-10-01 00:00:00

  • Depression of gustatory receptor potential in frog taste cell by parasympathetic nerve-induced slow hyperpolarizing potential.

    abstract::Parasympathetic nerve (PSN) innervates taste cells of the frog taste disk, and electrical stimulation of PSN elicited a slow hyperpolarizing potential (HP) in taste cells. Here we report that gustatory receptor potentials in frog taste cells are depressed by PSN-induced slow HPs. When PSN was stimulated at 30 Hz durin...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjl028

    authors: Sato T,Nishishita K,Mineda T,Okada Y,Toda K

    更新日期:2007-01-01 00:00:00

  • Decreased perception of bourgeonal may be linked to male idiopathic infertility.

    abstract::Regarding the chemotaxis of sperms, new insights have been gained during the last 20 years. Olfactory receptors are expressed on the flagellar midpiece of human spermatogenic cells. One of them, OR1D2, is also expressed in the olfactory epithelium. This receptor has been suggested to play a role in sperm chemotaxis an...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjt009

    authors: Sinding C,Kemper E,Spornraft-Ragaller P,Hummel T

    更新日期:2013-06-01 00:00:00

  • Olfactory Coding in Antennal Neurons of the Malaria Mosquito, Anopheles gambiae.

    abstract::Olfactory receptor neurons (ORNs) in the antenna of insects serve to encode odors in action potential activity conducted to the olfactory lobe of the deuterocerebrum. We performed an analysis of the electrophysiological responses of olfactory neurons in the antennae of the female malaria mosquito Anopheles gambiae s.s...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjl027

    authors: Qiu YT,van Loon JJ,Takken W,Meijerink J,Smid HM

    更新日期:2006-11-01 00:00:00

  • Olfactory signal modulation by molluscan cardioexcitatory tetrapeptide (FMRFamide) in axolotls (Ambystoma mexicanum).

    abstract::The terminal nerve, which innervates the nasal epithelia of most jawed vertebrates, is believed to release neuropeptides that modulate activity of sensory receptor neurons. The terminal nerve usually contains gonadotropin-releasing hormone as well as at least one other peptide that has not been characterized, but whic...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/28.4.339

    authors: Park D,Zawacki SR,Eisthen HL

    更新日期:2003-05-01 00:00:00

  • Bitter Fruit: Inverse Associations Between PTC and Antidesma bunius Perception.

    abstract::Ability to perceive the bitter compound phenylthiocarbamide (PTC) is inherited via a dominant "taster" allele of the TAS2R38 gene, whereas inability is inherited via a recessive "non-taster" allele. This raises a question: Is the non-taster allele functionless, or does it mediate perception of compounds other than PTC...

    journal_title:Chemical senses

    pub_type: 评论,杂志文章

    doi:10.1093/chemse/bjy044

    authors: Wooding SP

    更新日期:2018-08-24 00:00:00

  • Perception of everyday odors--correlation between intensity, familiarity and strength of hedonic judgement.

    abstract::In this study, 40 Japanese, 44 German and 39 Mexican women were presented with 18 everyday odorants. They were asked to rate them for intensity on a six-point scale from not detectable to very strong, for pleasantness on an 11-point scale from -5, to neutral at 0, to +5, and for familiarity on a six-point scale from c...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/24.2.191

    authors: Distel H,Ayabe-Kanamura S,Martínez-Gómez M,Schicker I,Kobayakawa T,Saito S,Hudson R

    更新日期:1999-04-01 00:00:00

  • The plant sesquiterpene germacrene D specifically activates a major type of antennal receptor neuron of the tobacco budworm moth Heliothis virescens.

    abstract::Plants release hundreds of volatiles that are important in interactions with insects or other organisms. However, knowledge is scarce as to which of the compounds are detected by the organism's olfactory receptor neurons. In the present study, single receptor neurons on the antennae of the tobacco budworm moth, Heliot...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/25.2.141

    authors: Røstelien T,Borg-Karlson AK,Fäldt J,Jacobsson U,Mustaparta H

    更新日期:2000-04-01 00:00:00

  • Ontogeny of the solitary chemosensory cells in the zebrafish, Danio rerio.

    abstract::Secondary epidermal solitary chemosensory cells (SCCs) are widespread among the primary aquatic vertebrates. They resemble taste bud sensory cells in fine structure and may be innervated from facial or spinal nerves. According to previous studies, SCCs may constitute a water sampling system in the contexts of predator...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/22.2.111

    authors: Kotrschal K,Krautgartner WD,Hansen A

    更新日期:1997-04-01 00:00:00

  • Taste solution preferences of C57BL/6J and 129X1/SvJ mice: influence of age, sex, and diet.

    abstract::To examine whether age influences taste solution preferences, we measured taste preferences of C57BL/6J and 129X1/SvJ mice given a series of 48-h 2-bottle tests with a choice between water and one of the following taste solutions: 2 mM saccharin, 5 mM citric acid, 30 microM quinine hydrochloride, 75 mM sodium chloride...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjm034

    authors: Tordoff MG

    更新日期:2007-09-01 00:00:00

  • Enhancement of Combined Umami and Salty Taste by Glutathione in the Human Tongue and Brain.

    abstract::Glutathione, a natural substance, acts on calcium receptors on the tongue and is known to enhance basic taste sensations. However, the effects of glutathione on brain activity associated with taste sensation on the tongue have not been determined under standardized taste delivery conditions. In this study, we investig...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjw066

    authors: Goto TK,Yeung AW,Tanabe HC,Ito Y,Jung HS,Ninomiya Y

    更新日期:2016-09-01 00:00:00

  • Distortion of olfactory perception: diagnosis and treatment.

    abstract::Clinically, olfaction can fail in any of three ways: (i) decreased sensitivity (hyposmia, anosmia) and two types of distortion (dysosmia); (ii) distorted quality of an odorant stimulation (troposmia); (iii) perceived odor when no odorant is present (phantosmia, hallucination). The distortions are usually much more ups...

    journal_title:Chemical senses

    pub_type: 杂志文章,评审

    doi:10.1093/chemse/27.7.611

    authors: Leopold D

    更新日期:2002-09-01 00:00:00

  • Distinct projections of two populations of olfactory receptor axons in the antennal lobe of the sphinx moth Manduca sexta.

    abstract::The central projections of olfactory receptor cells associated with two distinct types of antennal sensilla in the sphinx moth Manduca sexta were revealed by anterograde staining. In both sexes, receptor axons that arise from sexually isomorphic, type-II trichoid sensilla (and possibly some basiconic sensilla) project...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/20.3.313

    authors: Christensen TA,Harrow ID,Cuzzocrea C,Randolph PW,Hildebrand JG

    更新日期:1995-06-01 00:00:00

  • Derivation and evaluation of a labeled hedonic scale.

    abstract::The objective of this study was to develop a semantically labeled hedonic scale (LHS) that would yield ratio-level data on the magnitude of liking/disliking of sensation equivalent to that produced by magnitude estimation (ME). The LHS was constructed by having 49 subjects who were trained in ME rate the semantic magn...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjp054

    authors: Lim J,Wood A,Green BG

    更新日期:2009-11-01 00:00:00