Enucleation of demecolcine-treated bovine oocytes in cytochalasin-free medium: mechanism investigation and practical improvement.

abstract：:Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...

journal_title：Cellular reprogramming

authors： Wagner S,Cullum A,Wells DN,Laible G

更新日期：2017-12-01 00:00:00

abstract：:The generation of human induced pluripotent stem cells (hiPSCs) derived from an autologous extraembryonic fetal source is an innovative personalized regenerative technology that can transform own-self cells into embryonic stem-like ones. These cells are regarded as a promising candidate for cell-based therapy, as well...

journal_title：Cellular reprogramming

authors： Spitalieri P,Talarico RV,Botta A,Murdocca M,D'Apice MR,Orlandi A,Giardina E,Santoro M,Brancati F,Novelli G,Sangiuolo F

更新日期：2015-08-01 00:00:00

abstract：:Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of the donor cell. Previous studies suggest cellular heterogeneity among donor nuclei in regard to reprogramming potential, which precludes comparison among different strategies to increase cloning success...

journal_title：Cellular reprogramming

authors： Sá AL,Sampaio RV,da Costa Almeida NN,Sangalli JR,Brito KNL,Bressan FF,Rissino JD,do Socorro Damasceno Santos S,Meirelles FV,Ohashi OM,Dos Santos Miranda M

更新日期：2017-10-01 00:00:00

abstract：:The scope of this article is not to provide an exhaustive review of nuclear transfer research, because many authoritative reviews exist on the biological issues related to somatic and embryonic cell nuclear transfer. We shall instead provide an overview on the work done specifically on sheep and the value of this work...

journal_title：Cellular reprogramming

authors： Loi P,Czernik M,Zacchini F,Iuso D,Scapolo PA,Ptak G

更新日期：2013-10-01 00:00:00

abstract：:Chickens are vitally important in numerous countries as a primary food source and a major component of economic development. Efforts have been made to produce transgenic birds through pluripotent stem cell [primordial germ cells and embryonic stem cells (ESCs)] approaches to create animals with improved traits, such a...

journal_title：Cellular reprogramming

authors： Yu P,Lu Y,Jordan BJ,Liu Y,Yang JY,Hutcheson JM,Ethridge CL,Mumaw JL,Kinder HA,Beckstead RB,Stice SL,West FD

更新日期：2014-10-01 00:00:00

abstract：:Abstract Professor Keith Campbell's critical contribution to the discovery that a somatic cell from an adult animal can be fully reprogrammed by oocyte factors to form a cloned individual following nuclear transfer (NT)(Wilmut et al., 1997 ) overturned a dogma concerning the reversibility of cell fate that many scient...

journal_title：Cellular reprogramming

authors： Wells DN

更新日期：2013-10-01 00:00:00

abstract：:The purpose of this study was to investigate the effects of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on transgene expression and development of porcine transgenic cloned embryos, specifically focusing on effects derived from TSA-treated donor cells or TSA-treated reconstructed embryos. The results...

journal_title：Cellular reprogramming

authors： Luo B,Ju S,Muneri CW,Rui R

更新日期：2015-02-01 00:00:00

abstract：:Buffalo embryos were produced by hand-made cloning using skin fibroblasts from male and female buffaloes (n = 4 each) as donor cells for examining the effect of sex. Although the rate of blastocyst formation (43.8% ± 1.31% vs. 42.2% ± 1.22%) was similar, the total cell number (333 ± 10.4 vs. 270 ± 10.9) was higher (p ...

journal_title：Cellular reprogramming

authors： Sandhu A,Mohapatra SK,Agrawal H,Singh MK,Palta P,Singla SK,Chauhan MS,Manik RS

更新日期：2016-10-01 00:00:00

abstract：:A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons in vitro. It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small mol...

journal_title：Cellular reprogramming

authors： Qiu X,Liu Y,Xiao X,He J,Zhang H,Li Y

更新日期：2019-12-01 00:00:00

abstract：:The generation of induced pluripotent stem cells (iPSCs) from somatic cells by expressing ectopic reprogramming transcriptional factors such as Oct3/4, Sox2, Klf4, c-Myc, and Nanog is one of the cutting-edge discoveries in stem cell and cancer research. This discovery has raised several safety issues regarding the use...

journal_title：Cellular reprogramming

authors： Ramakrishna S,Kim KS,Baek KH

更新日期：2014-04-01 00:00:00

abstract：:Edaravone can induce differentiation of bone marrow mesenchymal stem cells (BMSCs) into neuron-like cells and replace lost cells by transplanting neuron-like cells to repair spinal cord injury (SCI). In this study, BMSCs were derived from the bone marrow of male Wistar rats (4 weeks old) through density gradient centr...

journal_title：Cellular reprogramming

authors： Li Y,Liu L,Yu Z,Yu Y,Sun B,Xiao C,Luo S,Li L

更新日期：2021-01-05 00:00:00

abstract：:The less differentiated the donor cells are used in nuclear transfer (NT), the more easily are they reprogrammed by the recipient cytoplasm. In this context, mesenchymal stem cells (MSCs) appear as an alternative to donor nuclei for NT. The amniotic fluid and adipose tissue are sources of MSCs that have not been teste...

journal_title：Cellular reprogramming

authors： da Silva CG,Martins CF,Cardoso TC,da Cunha ER,Bessler HC,Martins GH,Pivato I,Báo SN

更新日期：2016-04-01 00:00:00

abstract：:The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by t...

journal_title：Cellular reprogramming

authors： Rødgaard T,Skovgaard K,Stagsted J,Heegaard PM

更新日期：2012-10-01 00:00:00

abstract：:The present study compared the potential of porcine bone marrow mesenchymal stem cells (pBMSCs) at different passages as nuclear transfer (NT) donors and the developmental efficiency of NT embryos from donor cells transfected with/without Oct4 and Sox2. Early-passage pBMSCs showed higher proliferation and expression o...

journal_title：Cellular reprogramming

authors： Lee JH,Lee WJ,Jeon RH,Lee YM,Jang SJ,Lee SL,Jeon BG,Ock SA,King WA,Rho GJ

更新日期：2014-12-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this species. But such tools do not yet exist for many rat strains, especially for di...

journal_title：Cellular reprogramming

authors： Yang S,Ding S,Xu Q,Li X,Xiong Q

更新日期：2017-06-01 00:00:00

abstract：:Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lin...

journal_title：Cellular reprogramming

authors： Zhang L,Wang Y,Zhang Y,Wang L,Huang H

更新日期：2020-04-01 00:00:00

abstract：:Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression...

journal_title：Cellular reprogramming

authors： Wang X,Shi J,Cai G,Zheng E,Liu D,Wu Z,Li Z

更新日期：2019-10-01 00:00:00

abstract：:Methyl-CpG-binding domain proteins (MBPs) connect DNA methylation and histone modification, which are the key changes of somatic cell reprogramming. Methyl-CpG-binding protein 2 (MeCP2) was the first discovered MBP that has been extensively studied in the neurodevelopmental disorder Rett syndrome. However, a role for ...

journal_title：Cellular reprogramming

authors： Wang ZD,Duan L,Zhang ZH,Song SH,Bai GY,Zhang N,Shen XH,Shen JL,Lei L

更新日期：2016-04-01 00:00:00

abstract：:Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated wi...

journal_title：Cellular reprogramming

authors： Whitworth KM,Mao J,Lee K,Spollen WG,Samuel MS,Walters EM,Spate LD,Prather RS

更新日期：2015-08-01 00:00:00

abstract：:Skin-derived progenitors (SKP) are neural crest derived and can generate neural and mesodermal progeny in vitro, corresponding to the multipotency of neural crest stem cells. Likewise, neural stem/progenitor cells (displaying as neurospheres) have the capacity of self-renewing, and can produce most phenotypes in the n...

journal_title：Cellular reprogramming

authors： Zhao MT,Whitworth KM,Lin H,Zhang X,Isom SC,Dobbs KB,Bauer B,Zhang Y,Prather RS

更新日期：2010-06-01 00:00:00

abstract：:It has been demonstrated that several types of somatic stem cells have the remarkable capacity to differentiate into other types of tissues. However, the promise of keratinocyte stem cells seems slim for generating nonepidermal tissues. Using our recently developed acclimatization induction strategy, we demonstrate th...

journal_title：Cellular reprogramming

authors： Han W,Chen M,Li M,Wu Z,Zhao Y,Wang Y,Wang L,Yu L,Fu X

更新日期：2010-06-01 00:00:00

abstract：:Various diseases, injuries, and congenital abnormalities may result in degeneration and loss of organs and tissues. Recently, tissue engineering has offered new treatment options for these common, severe, and costly problems in human health care. Its application is often based on the usage of differentiated stem cells...

journal_title：Cellular reprogramming

authors： Dastagir K,Reimers K,Lazaridis A,Jahn S,Maurer V,Strauß S,Dastagir N,Radtke C,Kampmann A,Bucan V,Vogt PM

更新日期：2014-08-01 00:00:00

abstract：:The potential clinical applications of hematopoietic stem cells (HSCs) derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid hematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available ...

journal_title：Cellular reprogramming

authors： Tesarova L,Simara P,Stejskal S,Koutna I

更新日期：2017-08-01 00:00:00

abstract：:The development of embryos produced by somatic cell nuclear transfer (SCNT) using vitrified oocytes as cytoplast recipients has been reported in cattle but not in sheep. This study investigated the parthenogenetic development of ovine oocytes vitrified and thawed at the germinal vesicle (GV) stage, matured in vitro, a...

journal_title：Cellular reprogramming

authors： Moawad AR,Choi I,Zhu J,Campbell KH

更新日期：2011-08-01 00:00:00

abstract：:The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulatio...

journal_title：Cellular reprogramming

authors： Hosseini SM,Hajian M,Moulavi F,Asgari V,Forouzanfar M,Nasr-Esfahani MH

更新日期：2013-02-01 00:00:00

abstract：:In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...

journal_title：Cellular reprogramming

authors： Cravero D,Martignani E,Miretti S,Accornero P,Pauciullo A,Sharma R,Donadeu FX,Baratta M

更新日期：2015-06-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) play an important role in cell replacement therapy. Several studies have shown that keratinocytes are promising reprogrammed cells. We easily and efficiently enriched epidermal stem cells by attaching them for a limited time in culture dishes. Individual epidermal cells enriched ...

journal_title：Cellular reprogramming

authors： Yang YH,Zhang RZ,Cheng S,Xu B,Tian T,Shi HX,Xiao L,Chen RH

更新日期：2018-12-01 00:00:00

abstract：:Cell Stemness can be achieved by various reprogramming techniques namely, somatic cell nuclear transfer, cell fusion, cell extracts, and introduction of transcription factors from which induced pluripotent stem cells (iPSCs) are obtained. iPSCs are valuable cell sources for drug screening and human disease modeling. A...

journal_title：Cellular reprogramming

authors： Mirahmadi M,Nakhaei-Rad S,Matin MM,Shahriyari M,Saeinasab M,Mahmoudi Z,Haghighitalab A,Mahdavi-Shahri N,Bahrami AR

更新日期：2016-10-01 00:00:00

abstract：:In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as recepto...

journal_title：Cellular reprogramming

authors： Naaman H,Rabinski T,Yizhak A,Mizrahi S,Avni YS,Taube R,Rager B,Weinstein Y,Rall G,Gopas J,Ofir R

更新日期：2018-02-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) are promising candidates for the study of disease models as well as for tissue engineering purposes. Part of a strategy to develop safe reprogramming technique is reducing the number of exogenous reprogramming factors. Some cells types are more prone to reprogramming than others....

journal_title：Cellular reprogramming

authors： Ma MS,Czepiel M,Krause T,Schäfer KH,Boddeke E,Copray S

更新日期：2014-10-01 00:00:00