Dedifferentiation Effects of Rabbit Regenerating Tissue on Partially Differentiated Cells.

abstract：:Induced pluripotent stem cells (iPSCs) are promising candidates for the study of disease models as well as for tissue engineering purposes. Part of a strategy to develop safe reprogramming technique is reducing the number of exogenous reprogramming factors. Some cells types are more prone to reprogramming than others....

journal_title：Cellular reprogramming

authors： Ma MS,Czepiel M,Krause T,Schäfer KH,Boddeke E,Copray S

更新日期：2014-10-01 00:00:00

abstract：:Demecolcine-assisted/induced enucleation has been used in nuclear transfer cloning procedures for many species, yet its mechanism of action remains unclear. Primarily because oocytoplasm protrusion induced by demecolcine is inhibited by the presence of cytochalasin, its use has had limited application. In this experim...

journal_title：Cellular reprogramming

authors： Meng Q,Wu X,Bunch TD,White K,Sessions BR,Davies CJ,Rickords L,Li GP

更新日期：2011-10-01 00:00:00

abstract：:Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of ...

journal_title：Cellular reprogramming

authors： Sekhavati MH,Shadanloo F,Hosseini MS,Tahmoorespur M,Nasiri MR,Hajian M,Nasr-Esfahani MH

更新日期：2012-08-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) remain a promising approach to target diseases with a loss of functional parenchyma. This technology comes with a number of concerns for clinical applications, including teratogenic potential and genomic instability. Here we focused on evaluating the safety of cross-species Senda...

journal_title：Cellular reprogramming

authors： Tarnawski L,Eugster E,DeCamp L,Jovinge S

更新日期：2019-04-01 00:00:00

abstract：:Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated wi...

journal_title：Cellular reprogramming

authors： Whitworth KM,Mao J,Lee K,Spollen WG,Samuel MS,Walters EM,Spate LD,Prather RS

更新日期：2015-08-01 00:00:00

abstract：:The differentiation of multipotent stem cells toward a pancreatic lineage provides us with an alternative cell-based therapeutic approach to type 1 diabetes and enables us to study pancreas development. The current study aims to study the effect of growth factors such as activin A or nicotinamide, alone and in combina...

journal_title：Cellular reprogramming

authors： Balaji S,Zhou Y,Opara EC,Soker S

更新日期：2017-08-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) play an important role in cell replacement therapy. Several studies have shown that keratinocytes are promising reprogrammed cells. We easily and efficiently enriched epidermal stem cells by attaching them for a limited time in culture dishes. Individual epidermal cells enriched ...

journal_title：Cellular reprogramming

authors： Yang YH,Zhang RZ,Cheng S,Xu B,Tian T,Shi HX,Xiao L,Chen RH

更新日期：2018-12-01 00:00:00

abstract：:A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons in vitro. It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small mol...

journal_title：Cellular reprogramming

authors： Qiu X,Liu Y,Xiao X,He J,Zhang H,Li Y

更新日期：2019-12-01 00:00:00

abstract：:The less differentiated the donor cells are used in nuclear transfer (NT), the more easily are they reprogrammed by the recipient cytoplasm. In this context, mesenchymal stem cells (MSCs) appear as an alternative to donor nuclei for NT. The amniotic fluid and adipose tissue are sources of MSCs that have not been teste...

journal_title：Cellular reprogramming

authors： da Silva CG,Martins CF,Cardoso TC,da Cunha ER,Bessler HC,Martins GH,Pivato I,Báo SN

更新日期：2016-04-01 00:00:00

abstract：:Animal cloning by nuclear transfer (NT) has made the production of transgenic animals using genetically modified donor cells possible and ensures the presence of the gene construct in the offspring. The identification of transgene insertion sites in donor cells before cloning may avoid the production of animals that c...

journal_title：Cellular reprogramming

authors： Bressan FF,Dos Santos Miranda M,Perecin F,De Bem TH,Pereira FT,Russo-Carbolante EM,Alves D,Strauss B,Bajgelman M,Krieger JE,Binelli M,Meirelles FV

更新日期：2011-02-01 00:00:00

abstract：:Methyl-CpG-binding domain proteins (MBPs) connect DNA methylation and histone modification, which are the key changes of somatic cell reprogramming. Methyl-CpG-binding protein 2 (MeCP2) was the first discovered MBP that has been extensively studied in the neurodevelopmental disorder Rett syndrome. However, a role for ...

journal_title：Cellular reprogramming

authors： Wang ZD,Duan L,Zhang ZH,Song SH,Bai GY,Zhang N,Shen XH,Shen JL,Lei L

更新日期：2016-04-01 00:00:00

abstract：:The development of embryos produced by somatic cell nuclear transfer (SCNT) using vitrified oocytes as cytoplast recipients has been reported in cattle but not in sheep. This study investigated the parthenogenetic development of ovine oocytes vitrified and thawed at the germinal vesicle (GV) stage, matured in vitro, a...

journal_title：Cellular reprogramming

authors： Moawad AR,Choi I,Zhu J,Campbell KH

更新日期：2011-08-01 00:00:00

abstract：:In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as recepto...

journal_title：Cellular reprogramming

authors： Naaman H,Rabinski T,Yizhak A,Mizrahi S,Avni YS,Taube R,Rager B,Weinstein Y,Rall G,Gopas J,Ofir R

更新日期：2018-02-01 00:00:00

abstract：:Transgenic animal producing technology has improved consistently over the last couple of decades. Among the available methods, somatic cell nuclear transfer (SCNT) technology was officially the most popular. However, SCNT has low efficiency and requires a highly skilled individual. Additionally, the allo-SCNT nuclear ...

journal_title：Cellular reprogramming

authors： Hwang JH,Kim SE,Gupta MK,Lee H

更新日期：2016-08-01 00:00:00

abstract：:Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT. Co-culture with f...

journal_title：Cellular reprogramming

authors： Park MJ,Lee SE,Kim EY,Lee JB,Jeong CJ,Park SP

更新日期：2015-06-01 00:00:00

abstract：:Male germline stem cells (mGSCs) are stem cells present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived in vitro and resemble embryonic stem cells (ESCs) properties both in the mouse and humans. However, little is know about these cells in domestic a...

journal_title：Cellular reprogramming

authors： Hua J,Zhu H,Pan S,Liu C,Sun J,Ma X,Dong W,Liu W,Li W

更新日期：2011-04-01 00:00:00

abstract：:The purpose of this study was to investigate the effects of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on transgene expression and development of porcine transgenic cloned embryos, specifically focusing on effects derived from TSA-treated donor cells or TSA-treated reconstructed embryos. The results...

journal_title：Cellular reprogramming

authors： Luo B,Ju S,Muneri CW,Rui R

更新日期：2015-02-01 00:00:00

abstract：:Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...

journal_title：Cellular reprogramming

authors： Wagner S,Cullum A,Wells DN,Laible G

更新日期：2017-12-01 00:00:00

abstract：:It has been demonstrated that several types of somatic stem cells have the remarkable capacity to differentiate into other types of tissues. However, the promise of keratinocyte stem cells seems slim for generating nonepidermal tissues. Using our recently developed acclimatization induction strategy, we demonstrate th...

journal_title：Cellular reprogramming

authors： Han W,Chen M,Li M,Wu Z,Zhao Y,Wang Y,Wang L,Yu L,Fu X

更新日期：2010-06-01 00:00:00

abstract：:In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...

journal_title：Cellular reprogramming

authors： Cravero D,Martignani E,Miretti S,Accornero P,Pauciullo A,Sharma R,Donadeu FX,Baratta M

更新日期：2015-06-01 00:00:00

abstract：:The scope of this article is not to provide an exhaustive review of nuclear transfer research, because many authoritative reviews exist on the biological issues related to somatic and embryonic cell nuclear transfer. We shall instead provide an overview on the work done specifically on sheep and the value of this work...

journal_title：Cellular reprogramming

authors： Loi P,Czernik M,Zacchini F,Iuso D,Scapolo PA,Ptak G

更新日期：2013-10-01 00:00:00

abstract：:Although numerous mammalian species have been successfully cloned by somatic cell nuclear transfer (SCNT), little is known about gene expression of cloned pigs by SCNT. In the present study, expression profiles of 1-month-old cloned pigs generated from fetal fibroblasts (n = 5) were compared to those of age-matched co...

journal_title：Cellular reprogramming

authors： Park J,Lai L,Samuel M,Wax D,Bruno RS,French R,Prather RS,Yang X,Tian XC

更新日期：2011-06-01 00:00:00

abstract：:Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned embryos transferred to an individual surrogate are two major factors that affect the successful rate of somatic cell nuclear transfer (SCNT) in pigs. This study aimed to compare the influence of different donor fibroblast cell type...

journal_title：Cellular reprogramming

authors： Li Z,Shi J,Liu D,Zhou R,Zeng H,Zhou X,Mai R,Zeng S,Luo L,Yu W,Zhang S,Wu Z

更新日期：2013-02-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this species. But such tools do not yet exist for many rat strains, especially for di...

journal_title：Cellular reprogramming

authors： Yang S,Ding S,Xu Q,Li X,Xiong Q

更新日期：2017-06-01 00:00:00

abstract：:Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndMT) describe complex changes in progenitor lineage, cell morphology, and gene expression. Stimulated by environmental cues, these cellular transitions are essential for elements of embryonic development and can be pathologically d...

journal_title：Cellular reprogramming

authors： Schindeler A,Kolind M,Little DG

更新日期：2013-04-01 00:00:00

abstract：:Based on microarray data comparing gene expression of fibroblast donor cells and bovine somatic cell nuclear transfer (SCNT) and in vivo produced (AI) blastocysts, a group of genes including several transcription factors was selected for evaluation of transcript abundance. Using SYBR green-based real-time polymerase c...

journal_title：Cellular reprogramming

authors： Aston KI,Li GP,Hicks BA,Sessions BR,Davis AP,Rickords LF,Stevens JR,White KL

更新日期：2010-02-01 00:00:00

abstract：:Various diseases, injuries, and congenital abnormalities may result in degeneration and loss of organs and tissues. Recently, tissue engineering has offered new treatment options for these common, severe, and costly problems in human health care. Its application is often based on the usage of differentiated stem cells...

journal_title：Cellular reprogramming

authors： Dastagir K,Reimers K,Lazaridis A,Jahn S,Maurer V,Strauß S,Dastagir N,Radtke C,Kampmann A,Bucan V,Vogt PM

更新日期：2014-08-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) are generated from somatic cells through ectopic expression of defined transcription factors. So far, many iPSC lines have been established in various species, including porcine. However, the molecular events during somatic cell reprogramming in pig are largely unknown. The aim o...

journal_title：Cellular reprogramming

authors： Cheng D,Li Z,Liu Y,Gao Y,Wang H

更新日期：2012-08-01 00:00:00

abstract：:Induced pluripotent stem cell (iPSC) technology has become an important tool for disease modeling. Insufficient data on the variability among iPSC lines derived from a single somatic parental cell line have in practice led to generation and analysis of several, usually three, iPSC sister lines from each parental cell ...

journal_title：Cellular reprogramming

authors： Schuster J,Halvardson J,Pilar Lorenzo L,Ameur A,Sobol M,Raykova D,Annerén G,Feuk L,Dahl N

更新日期：2015-10-01 00:00:00

abstract：:Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lin...

journal_title：Cellular reprogramming

authors： Zhang L,Wang Y,Zhang Y,Wang L,Huang H

更新日期：2020-04-01 00:00:00