Abstract:
:The scope of this article is not to provide an exhaustive review of nuclear transfer research, because many authoritative reviews exist on the biological issues related to somatic and embryonic cell nuclear transfer. We shall instead provide an overview on the work done specifically on sheep and the value of this work on the greater nuclear transfer landscape.
journal_name
Cell Reprogramjournal_title
Cellular reprogrammingauthors
Loi P,Czernik M,Zacchini F,Iuso D,Scapolo PA,Ptak Gdoi
10.1089/cell.2013.0032subject
Has Abstractpub_date
2013-10-01 00:00:00pages
367-73issue
5eissn
2152-4971issn
2152-4998journal_volume
15pub_type
杂志文章,评审abstract::Methyl-CpG-binding domain proteins (MBPs) connect DNA methylation and histone modification, which are the key changes of somatic cell reprogramming. Methyl-CpG-binding protein 2 (MeCP2) was the first discovered MBP that has been extensively studied in the neurodevelopmental disorder Rett syndrome. However, a role for ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0060
更新日期:2016-04-01 00:00:00
abstract::Recent work has shown that mouse and human fibroblasts can be reprogrammed to cardiomyocyte-like cells with a combination of transcription factors. Current research has focused on improving the efficiency and mechanisms for fibroblast reprogramming. Previously, it has been reported that hypoxia enhances fibroblast cel...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0051
更新日期:2016-02-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) remain a promising approach to target diseases with a loss of functional parenchyma. This technology comes with a number of concerns for clinical applications, including teratogenic potential and genomic instability. Here we focused on evaluating the safety of cross-species Senda...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2018.0048
更新日期:2019-04-01 00:00:00
abstract::In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0087
更新日期:2015-06-01 00:00:00
abstract::The present study compared the potential of porcine bone marrow mesenchymal stem cells (pBMSCs) at different passages as nuclear transfer (NT) donors and the developmental efficiency of NT embryos from donor cells transfected with/without Oct4 and Sox2. Early-passage pBMSCs showed higher proliferation and expression o...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0036
更新日期:2014-12-01 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndMT) describe complex changes in progenitor lineage, cell morphology, and gene expression. Stimulated by environmental cues, these cellular transitions are essential for elements of embryonic development and can be pathologically d...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2012.0054
更新日期:2013-04-01 00:00:00
abstract::A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons in vitro. It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small mol...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0037
更新日期:2019-12-01 00:00:00
abstract::Chicken embryonic stem cells (cESCs) isolated from the egg at the stage X hold great promise for cell therapy, tissue engineering, pharmaceutical, and biotechnological applications. They are considered to be pluripotent cells with the capacity to self-renewal and differentiate into specialized cells. However, long-ter...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0080
更新日期:2020-04-01 00:00:00
abstract::Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated wi...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0022
更新日期:2015-08-01 00:00:00
abstract::The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulatio...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0033
更新日期:2013-02-01 00:00:00
abstract::Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells as donors...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0021
更新日期:2017-12-01 00:00:00
abstract::Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned embryos transferred to an individual surrogate are two major factors that affect the successful rate of somatic cell nuclear transfer (SCNT) in pigs. This study aimed to compare the influence of different donor fibroblast cell type...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0042
更新日期:2013-02-01 00:00:00
abstract::Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0018
更新日期:2017-12-01 00:00:00
abstract::The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by t...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0026
更新日期:2012-10-01 00:00:00
abstract::Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT. Co-culture with f...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0072
更新日期:2015-06-01 00:00:00
abstract::The generation of induced pluripotent stem cells (iPSCs) from somatic cells by expressing ectopic reprogramming transcriptional factors such as Oct3/4, Sox2, Klf4, c-Myc, and Nanog is one of the cutting-edge discoveries in stem cell and cancer research. This discovery has raised several safety issues regarding the use...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0077
更新日期:2014-04-01 00:00:00
abstract::The differentiation of multipotent stem cells toward a pancreatic lineage provides us with an alternative cell-based therapeutic approach to type 1 diabetes and enables us to study pancreas development. The current study aims to study the effect of growth factors such as activin A or nicotinamide, alone and in combina...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0043
更新日期:2017-08-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) are generated from somatic cells through ectopic expression of defined transcription factors. So far, many iPSC lines have been established in various species, including porcine. However, the molecular events during somatic cell reprogramming in pig are largely unknown. The aim o...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0025
更新日期:2012-08-01 00:00:00
abstract::The generation of human induced pluripotent stem cells (hiPSCs) derived from an autologous extraembryonic fetal source is an innovative personalized regenerative technology that can transform own-self cells into embryonic stem-like ones. These cells are regarded as a promising candidate for cell-based therapy, as well...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0003
更新日期:2015-08-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) are promising candidates for the study of disease models as well as for tissue engineering purposes. Part of a strategy to develop safe reprogramming technique is reducing the number of exogenous reprogramming factors. Some cells types are more prone to reprogramming than others....
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0018
更新日期:2014-10-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this species. But such tools do not yet exist for many rat strains, especially for di...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0028
更新日期:2017-06-01 00:00:00
abstract::It has been demonstrated that several types of somatic stem cells have the remarkable capacity to differentiate into other types of tissues. However, the promise of keratinocyte stem cells seems slim for generating nonepidermal tissues. Using our recently developed acclimatization induction strategy, we demonstrate th...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0095
更新日期:2010-06-01 00:00:00
abstract::Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of the donor cell. Previous studies suggest cellular heterogeneity among donor nuclei in regard to reprogramming potential, which precludes comparison among different strategies to increase cloning success...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0063
更新日期:2017-10-01 00:00:00
abstract::Abstract Professor Keith Campbell's critical contribution to the discovery that a somatic cell from an adult animal can be fully reprogrammed by oocyte factors to form a cloned individual following nuclear transfer (NT)(Wilmut et al., 1997 ) overturned a dogma concerning the reversibility of cell fate that many scient...
journal_title:Cellular reprogramming
pub_type: 传,历史文章,杂志文章
doi:10.1089/cell.2013.0038
更新日期:2013-10-01 00:00:00
abstract::The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-t...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0044
更新日期:2015-12-01 00:00:00
abstract::Various diseases, injuries, and congenital abnormalities may result in degeneration and loss of organs and tissues. Recently, tissue engineering has offered new treatment options for these common, severe, and costly problems in human health care. Its application is often based on the usage of differentiated stem cells...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0005
更新日期:2014-08-01 00:00:00
abstract::Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0014
更新日期:2012-08-01 00:00:00
abstract::The development of embryos produced by somatic cell nuclear transfer (SCNT) using vitrified oocytes as cytoplast recipients has been reported in cattle but not in sheep. This study investigated the parthenogenetic development of ovine oocytes vitrified and thawed at the germinal vesicle (GV) stage, matured in vitro, a...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0089
更新日期:2011-08-01 00:00:00
abstract::In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as recepto...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0034
更新日期:2018-02-01 00:00:00
abstract::The potential clinical applications of hematopoietic stem cells (HSCs) derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid hematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0042
更新日期:2017-08-01 00:00:00