Abstract:
:Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated with suberoylanilide hydroxamic acid (SAHA), 4-iodo-SAHA (ISAHA), or Scriptaid as compared to untreated SCNT, in vitro-fertilized (IVF), and in vivo (IVV) blastocyst-stage embryos. SAHA (10 μM) had the highest level of blastocyst development at 43.9%, and all treatments except 10 μM ISAHA had the same percentage of blastocyst development as Scriptaid (p<0.05). Two treatments, 1.0 μM ISAHA and 1.0 μM SAHA, had higher mean cell number than No HDACi treatment (p<0.021). Embryo transfers performed with 10 μM SAHA- and 1 μM ISAHA-treated embryos resulted in the birth of healthy piglets. GenBank accession numbers from up- and downregulated transcripts were loaded into the Database for Annotation, Visualization and Integrated Discovery to identify enriched biological themes. HDACi treatment yielded the highest enrichment for transcripts within the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway, lysosome. The mean intensity of LysoTracker was lower in IVV embryos compared to IVF and SCNT embryos (p<0.0001). SAHA and ISAHA can successfully be used to create healthy piglets from SCNT.
journal_name
Cell Reprogramjournal_title
Cellular reprogrammingauthors
Whitworth KM,Mao J,Lee K,Spollen WG,Samuel MS,Walters EM,Spate LD,Prather RSdoi
10.1089/cell.2015.0022subject
Has Abstractpub_date
2015-08-01 00:00:00pages
243-58issue
4eissn
2152-4971issn
2152-4998journal_volume
17pub_type
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0009
更新日期:2015-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 传,历史文章,杂志文章
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更新日期:2013-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0065
更新日期:2016-08-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0028
更新日期:2014-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0018
更新日期:2017-12-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0022
更新日期:2011-02-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2018.0048
更新日期:2019-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0083
更新日期:2020-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0064
更新日期:2016-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
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更新日期:2013-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
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更新日期:2013-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0065
更新日期:2013-06-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0061
更新日期:2017-06-01 00:00:00