Abstract:
:Adult cardiomyocytes have little ability to regenerate, thus cardiac regeneration therapy represents a potential method for treating severe heart failure. Human amniotic mesenchymal cells (hAMCs) have the potential to be a useful cell source for cardiac regeneration therapy. We attempted to isolate stem cells from hAMCs and differentiate them into cardiomyocytes. Nanog promoter-Cre plasmid and cytomegalovirus (CMV) promoter-loxP-STOP-loxP-Red-puro(r) plasmid were co-transfected into immortalized hAMCs (iHAMs). Nanog-positive iHAMs were treated with 5-azacytidine (5-aza), trichostatin A (TA), activin A (AA), and bone morphogenetic protein-4 (BMP-4), or co-cultured with murine fetal cardiomyocytes for cardiomyocytes differentiation. Isolated Nanog-positive iHAMs were analyzed by quantitative RT-PCR and immunofluorescent staining before and after differentiation. Expression of Nanog, Oct3/4, Sox2, and Klf4 was significantly higher in Nanog-positive than in Nanog-negative iHAMs. Nanog-positive iHAMs were stained for Nanog and Oct3/4 in the nucleus. Nanog-positive iHAMs treated with 5-aza expressed Nkx2.5, GATA-4, human atrial natriuretic peptide (hANP), cardiac troponin T (cTnT), myocin light chain (Mlc)-2a, Mlc-2v, β-myosin heavy chain (β-MHC), hyperpolarization-activated cyclic nucleotide gated channels (HCN)-4, and inwardly rectifying potassium channels (Kir)-2.1. Although Nanog-positive iHAMs treated with TA, AA, or BMP-4 expressed several cardiac markers, no contraction was observed. Co-cultured Nanog-positive iHAMs with murine fetal cardiomyocytes spontaneously contracted in a synchronized manner and expressed the cardiac markers. In conclusion, Nanog-positive hAMCs with characteristics of stem cells were isolated and differentiated into cardiomyocyte-like cells, suggesting that these isolated hAMCs could be a useful cell source for cardiac regeneration therapy.
journal_name
Cell Reprogramjournal_title
Cellular reprogrammingauthors
Otaka S,Nagura S,Koike C,Okabe M,Yoshida T,Fathy M,Yanagi K,Misaki T,Nikaido Tdoi
10.1089/cell.2012.0028subject
Has Abstractpub_date
2013-02-01 00:00:00pages
80-91issue
1eissn
2152-4971issn
2152-4998journal_volume
15pub_type
杂志文章abstract::The differentiation of multipotent stem cells toward a pancreatic lineage provides us with an alternative cell-based therapeutic approach to type 1 diabetes and enables us to study pancreas development. The current study aims to study the effect of growth factors such as activin A or nicotinamide, alone and in combina...
journal_title:Cellular reprogramming
pub_type: 杂志文章
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abstract::The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-t...
journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2018-12-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0018
更新日期:2014-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0041
更新日期:2015-02-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0047
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0077
更新日期:2014-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0064
更新日期:2016-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0042
更新日期:2010-02-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0065
更新日期:2013-06-01 00:00:00
abstract::In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0087
更新日期:2015-06-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0025
更新日期:2012-08-01 00:00:00
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0077
更新日期:2016-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2011.0067
更新日期:2012-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章,收录出版
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
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