Abstract:
:Skin-derived progenitors (SKP) are neural crest derived and can generate neural and mesodermal progeny in vitro, corresponding to the multipotency of neural crest stem cells. Likewise, neural stem/progenitor cells (displaying as neurospheres) have the capacity of self-renewing, and can produce most phenotypes in the nervous system. Both form spheres when cultured with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). Although the "stemness" of neural stem/progenitor cells has been extensively investigated, the molecular comparison of SKP spheres and neurospheres has not been elucidated. Here, SKP spheres and neurospheres from the same individual porcine fetuses were isolated with the same culture medium, and the multipotency was tested by in vitro differentiation assays. Microarray analysis was used to illustrate the "stemness" of SKP spheres and neurospheres. The upregulated genes that were in common in the SKP spheres and neurospheres are involved in ribosome, tight junction, gap junction, cell communication, calcium signaling, ErbB signaling, JAK-STAT signaling, MAPK signaling, etc. The differentially expressed genes between SKP spheres and neurospheres are mainly involved in ECM-receptor interaction and the transforming growth factor-beta (TGF-b) signaling pathway. Finally, treatment with leukemia inhibitory factor (LIF) or MEK inhibitor results in a distinctive impact on the "stemness" and differentiation genes of SKP spheres and neurospheres. Thus, the cell-intrinsic genetic program may contribute to the innate "stemness" of SKP spheres and neurospheres in a similar local microenvironment.
journal_name
Cell Reprogramjournal_title
Cellular reprogrammingauthors
Zhao MT,Whitworth KM,Lin H,Zhang X,Isom SC,Dobbs KB,Bauer B,Zhang Y,Prather RSdoi
10.1089/cell.2009.0116subject
Has Abstractpub_date
2010-06-01 00:00:00pages
329-45issue
3eissn
2152-4971issn
2152-4998journal_volume
12pub_type
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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abstract::The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-t...
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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abstract::Induced pluripotent stem cells (iPSCs) are promising candidates for the study of disease models as well as for tissue engineering purposes. Part of a strategy to develop safe reprogramming technique is reducing the number of exogenous reprogramming factors. Some cells types are more prone to reprogramming than others....
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0018
更新日期:2014-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0032
更新日期:2013-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0021
更新日期:2017-12-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0036
更新日期:2014-12-01 00:00:00
abstract::The development of embryos produced by somatic cell nuclear transfer (SCNT) using vitrified oocytes as cytoplast recipients has been reported in cattle but not in sheep. This study investigated the parthenogenetic development of ovine oocytes vitrified and thawed at the germinal vesicle (GV) stage, matured in vitro, a...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0089
更新日期:2011-08-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0042
更新日期:2013-02-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0047
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0060
更新日期:2016-04-01 00:00:00
abstract::In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0087
更新日期:2015-06-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0042
更新日期:2010-02-01 00:00:00
abstract::Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0018
更新日期:2017-12-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0005
更新日期:2014-08-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0034
更新日期:2018-02-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章,收录出版
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更新日期:2018-12-27 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
abstract::Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of the donor cell. Previous studies suggest cellular heterogeneity among donor nuclei in regard to reprogramming potential, which precludes comparison among different strategies to increase cloning success...
journal_title:Cellular reprogramming
pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0077
更新日期:2014-04-01 00:00:00
abstract::The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulatio...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0033
更新日期:2013-02-01 00:00:00
abstract::Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT. Co-culture with f...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0072
更新日期:2015-06-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0052
更新日期:2013-04-01 00:00:00
abstract::Demecolcine-assisted/induced enucleation has been used in nuclear transfer cloning procedures for many species, yet its mechanism of action remains unclear. Primarily because oocytoplasm protrusion induced by demecolcine is inhibited by the presence of cytochalasin, its use has had limited application. In this experim...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2011.0012
更新日期:2011-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2020.0055
更新日期:2021-01-05 00:00:00