Abstract:
:Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells as donors and rabbit oocytes as recipients, we show that Oct4 and other pluripotency related genes were reactivated in some of mouse-rabbit iSCNT embryos, which could also activate Oct4 promoter-driven GFP reporter gene expression. Series nuclear transfer improved the efficiency of Oct4 reactivation. DNA demethylation of Oct4 promoter was detected in GFP positive iSCNT blastocysts, whereas GFP negative iSCNT embryos showed a low efficiency. Our results demonstrate that Oct4-GFP can well label the embryos with epigenetic remodeling and reactivation of pluripotent gene expression. Abundant rabbit mitochondria specific DNAs were identified in reconstructed mouse-rabbit embryos throughout preimplantation stages. Our data demonstrate that epigenetic remodeling and the complete mitochondrial match are not necessary for successful iSCNT embryo development before implantation.
journal_name
Cell Reprogramjournal_title
Cellular reprogrammingauthors
Huang X,Song L,Zhan Z,Gu H,Feng H,Li Ydoi
10.1089/cell.2017.0021subject
Has Abstractpub_date
2017-12-01 00:00:00pages
344-353issue
6eissn
2152-4971issn
2152-4998journal_volume
19pub_type
杂志文章abstract::Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of the donor cell. Previous studies suggest cellular heterogeneity among donor nuclei in regard to reprogramming potential, which precludes comparison among different strategies to increase cloning success...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0063
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journal_title:Cellular reprogramming
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1089/cell.2015.0009
更新日期:2015-10-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0032
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abstract::Previous studies have shown that the time interval between fusion and activation (FA interval) play an important role in nuclear remodeling and in vitro development of somatic cell nuclear transfer (SCNT) embryos. However, the effects of FA interval on the epigenetic reprogramming and in vivo developmental competence ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0025
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0008
更新日期:2016-10-01 00:00:00
abstract::Chicken embryonic stem cells (cESCs) isolated from the egg at the stage X hold great promise for cell therapy, tissue engineering, pharmaceutical, and biotechnological applications. They are considered to be pluripotent cells with the capacity to self-renewal and differentiate into specialized cells. However, long-ter...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0080
更新日期:2020-04-01 00:00:00
abstract::Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lin...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0083
更新日期:2020-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0077
更新日期:2014-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0028
更新日期:2017-06-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0065
更新日期:2016-08-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0037
更新日期:2019-12-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2011.0067
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abstract::In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0042
更新日期:2010-02-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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journal_title:Cellular reprogramming
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doi:10.1089/cell.2020.0055
更新日期:2021-01-05 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
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journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
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更新日期:2013-04-01 00:00:00
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pub_type: 传,历史文章,杂志文章
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