Development and gene expression of porcine cloned embryos derived from bone marrow stem cells with overexpressing Oct4 and Sox2.

Abstract:

:The present study compared the potential of porcine bone marrow mesenchymal stem cells (pBMSCs) at different passages as nuclear transfer (NT) donors and the developmental efficiency of NT embryos from donor cells transfected with/without Oct4 and Sox2. Early-passage pBMSCs showed higher proliferation and expression of Oct4 and Sox2 and differentiation potential into mesenchymal lineages than middle- and late-passage pBMSCs. Cleavage rate did not differ among pBMSCs at different passages, but NT embryos with early-passage pBMSCs and middle-passage pBMSCs transfected with Oct4 (Oct4-pBMSCs) had significantly (p<0.05) higher blastocyst development than those with middle-passage pBMSCs. The incidence of apoptotic bodies in NT blastocysts from late-passage pBMSCs and Sox2-transfected middle-passage pBMSCs (Sox2-pBMSCs) was significantly (p<0.05) higher than others. The transcriptional levels of Oct4, Sox2, Nanog, Cdx2, Dnmt3a, and Igf2r genes were significantly (p<0.05) higher in Oct4- and Sox2-pBMSCs NT embryos. Middle-passage pBMSCs NT embryos revealed lower transcriptional levels of Bcl2 than others, except Sox2-pBMSCs NT embryos. The transcriptional level of Bax increased gradually in NT embryos derived from pBMSCs following extended passages and was significantly (p<0.05) higher in Sox2-pBMSCs NT embryos. Our results demonstrated that early-passage pBMSCs are more potent in expressing transcription factors and displayed higher differentiation ability, and middle-passage pBMSCs transfected with Oct4 improved the developmental efficiency of NT embryos, suggesting that high Oct4 expression cells are more efficient as NT donors.

journal_name

Cell Reprogram

journal_title

Cellular reprogramming

authors

Lee JH,Lee WJ,Jeon RH,Lee YM,Jang SJ,Lee SL,Jeon BG,Ock SA,King WA,Rho GJ

doi

10.1089/cell.2014.0036

subject

Has Abstract

pub_date

2014-12-01 00:00:00

pages

428-38

issue

6

eissn

2152-4971

issn

2152-4998

journal_volume

16

pub_type

杂志文章
  • Ovine oocytes vitrified at germinal vesicle stage as cytoplast recipients for somatic cell nuclear transfer (SCNT).

    abstract::The development of embryos produced by somatic cell nuclear transfer (SCNT) using vitrified oocytes as cytoplast recipients has been reported in cattle but not in sheep. This study investigated the parthenogenetic development of ovine oocytes vitrified and thawed at the germinal vesicle (GV) stage, matured in vitro, a...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2010.0089

    authors: Moawad AR,Choi I,Zhu J,Campbell KH

    更新日期:2011-08-01 00:00:00

  • Improved bovine ICSI outcomes by sperm selected after combined heparin-glutathione treatment.

    abstract::Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of ...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2012.0014

    authors: Sekhavati MH,Shadanloo F,Hosseini MS,Tahmoorespur M,Nasiri MR,Hajian M,Nasr-Esfahani MH

    更新日期:2012-08-01 00:00:00

  • Gnotobiotic Miniature Pig Interbreed Somatic Cell Nuclear Transfer for Xenotransplantation.

    abstract::Transgenic animal producing technology has improved consistently over the last couple of decades. Among the available methods, somatic cell nuclear transfer (SCNT) technology was officially the most popular. However, SCNT has low efficiency and requires a highly skilled individual. Additionally, the allo-SCNT nuclear ...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2015.0065

    authors: Hwang JH,Kim SE,Gupta MK,Lee H

    更新日期:2016-08-01 00:00:00

  • Effect of Sex of Embryo on Developmental Competence, Epigenetic Status, and Gene Expression in Buffalo (Bubalus bubalis) Embryos Produced by Hand-Made Cloning.

    abstract::Buffalo embryos were produced by hand-made cloning using skin fibroblasts from male and female buffaloes (n = 4 each) as donor cells for examining the effect of sex. Although the rate of blastocyst formation (43.8% ± 1.31% vs. 42.2% ± 1.22%) was similar, the total cell number (333 ± 10.4 vs. 270 ± 10.9) was higher (p ...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2015.0077

    authors: Sandhu A,Mohapatra SK,Agrawal H,Singh MK,Palta P,Singla SK,Chauhan MS,Manik RS

    更新日期:2016-10-01 00:00:00

  • Overexpression of MBD3 Improves Reprogramming of Cloned Pig Embryos.

    abstract::Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2019.0008

    authors: Wang X,Shi J,Cai G,Zheng E,Liu D,Wu Z,Li Z

    更新日期:2019-10-01 00:00:00

  • Nonviral minicircle generation of induced pluripotent stem cells compatible with production of chimeric chickens.

    abstract::Chickens are vitally important in numerous countries as a primary food source and a major component of economic development. Efforts have been made to produce transgenic birds through pluripotent stem cell [primordial germ cells and embryonic stem cells (ESCs)] approaches to create animals with improved traits, such a...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2014.0028

    authors: Yu P,Lu Y,Jordan BJ,Liu Y,Yang JY,Hutcheson JM,Ethridge CL,Mumaw JL,Kinder HA,Beckstead RB,Stice SL,West FD

    更新日期:2014-10-01 00:00:00

  • Generation and characterization of neurospheres from canine adipose tissue-derived stromal cells.

    abstract::Adipose tissue-derived stromal cells (ADSCs) have been identified as a powerful stem cell source for cellular transplantation therapy. The dog is increasingly used as a model of human neurological disease; however, few studies have reported induction of canine ADSCs to neural lineages. We characterized canine ADSCs an...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2009.0093

    authors: Lim JH,Boozer L,Mariani CL,Piedrahita JA,Olby NJ

    更新日期:2010-08-01 00:00:00

  • Intermediate Standstill Clones Trapped in the Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells.

    abstract::Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lin...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2019.0083

    authors: Zhang L,Wang Y,Zhang Y,Wang L,Huang H

    更新日期:2020-04-01 00:00:00

  • Genetic Manipulation by Zinc-Finger Nucleases in Rat-Induced Pluripotent Stem Cells.

    abstract::Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this species. But such tools do not yet exist for many rat strains, especially for di...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2016.0028

    authors: Yang S,Ding S,Xu Q,Li X,Xiong Q

    更新日期:2017-06-01 00:00:00

  • Effect of POU5F1 Expression Level in Clonal Subpopulations of Bovine Fibroblasts Used as Nuclear Donors for Somatic Cell Nuclear Transfer.

    abstract::Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of the donor cell. Previous studies suggest cellular heterogeneity among donor nuclei in regard to reprogramming potential, which precludes comparison among different strategies to increase cloning success...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2016.0063

    authors: Sá AL,Sampaio RV,da Costa Almeida NN,Sangalli JR,Brito KNL,Bressan FF,Rissino JD,do Socorro Damasceno Santos S,Meirelles FV,Ohashi OM,Dos Santos Miranda M

    更新日期:2017-10-01 00:00:00

  • Taillessness in a Cloned Cow is Not Genetically Transmitted.

    abstract::Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2017.0018

    authors: Wagner S,Cullum A,Wells DN,Laible G

    更新日期:2017-12-01 00:00:00

  • Transcriptome Analysis of Pig In Vivo, In Vitro-Fertilized, and Nuclear Transfer Blastocyst-Stage Embryos Treated with Histone Deacetylase Inhibitors Postfusion and Activation Reveals Changes in the Lysosomal Pathway.

    abstract::Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated wi...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2015.0022

    authors: Whitworth KM,Mao J,Lee K,Spollen WG,Samuel MS,Walters EM,Spate LD,Prather RS

    更新日期:2015-08-01 00:00:00

  • Improved production of genetically modified fetuses with homogeneous transgene expression after transgene integration site analysis and recloning in cattle.

    abstract::Animal cloning by nuclear transfer (NT) has made the production of transgenic animals using genetically modified donor cells possible and ensures the presence of the gene construct in the offspring. The identification of transgene insertion sites in donor cells before cloning may avoid the production of animals that c...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2010.0022

    authors: Bressan FF,Dos Santos Miranda M,Perecin F,De Bem TH,Pereira FT,Russo-Carbolante EM,Alves D,Strauss B,Bajgelman M,Krieger JE,Binelli M,Meirelles FV

    更新日期:2011-02-01 00:00:00

  • Generation of Induced Pluripotent Stem Cells from Human Epidermal Keratinocytes.

    abstract::Induced pluripotent stem cells (iPSCs) play an important role in cell replacement therapy. Several studies have shown that keratinocytes are promising reprogrammed cells. We easily and efficiently enriched epidermal stem cells by attaching them for a limited time in culture dishes. Individual epidermal cells enriched ...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2018.0035

    authors: Yang YH,Zhang RZ,Cheng S,Xu B,Tian T,Shi HX,Xiao L,Chen RH

    更新日期:2018-12-01 00:00:00

  • Abnormal levels of transcript abundance of developmentally important genes in various stages of preimplantation bovine somatic cell nuclear transfer embryos.

    abstract::Based on microarray data comparing gene expression of fibroblast donor cells and bovine somatic cell nuclear transfer (SCNT) and in vivo produced (AI) blastocysts, a group of genes including several transcription factors was selected for evaluation of transcript abundance. Using SYBR green-based real-time polymerase c...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2009.0042

    authors: Aston KI,Li GP,Hicks BA,Sessions BR,Davis AP,Rickords LF,Stevens JR,White KL

    更新日期:2010-02-01 00:00:00

  • Selective isolation of nanog-positive human amniotic mesenchymal cells and differentiation into cardiomyocytes.

    abstract::Adult cardiomyocytes have little ability to regenerate, thus cardiac regeneration therapy represents a potential method for treating severe heart failure. Human amniotic mesenchymal cells (hAMCs) have the potential to be a useful cell source for cardiac regeneration therapy. We attempted to isolate stem cells from hAM...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2012.0028

    authors: Otaka S,Nagura S,Koike C,Okabe M,Yoshida T,Fathy M,Yanagi K,Misaki T,Nikaido T

    更新日期:2013-02-01 00:00:00

  • Methyl-CpG-Binding Protein 2 Improves the Development of Mouse Somatic Cell Nuclear Transfer Embryos.

    abstract::Methyl-CpG-binding domain proteins (MBPs) connect DNA methylation and histone modification, which are the key changes of somatic cell reprogramming. Methyl-CpG-binding protein 2 (MeCP2) was the first discovered MBP that has been extensively studied in the neurodevelopmental disorder Rett syndrome. However, a role for ...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2015.0060

    authors: Wang ZD,Duan L,Zhang ZH,Song SH,Bai GY,Zhang N,Shen XH,Shen JL,Lei L

    更新日期:2016-04-01 00:00:00

  • Effect of the time interval between fusion and activation on epigenetic reprogramming and development of bovine somatic cell nuclear transfer embryos.

    abstract::Previous studies have shown that the time interval between fusion and activation (FA interval) play an important role in nuclear remodeling and in vitro development of somatic cell nuclear transfer (SCNT) embryos. However, the effects of FA interval on the epigenetic reprogramming and in vivo developmental competence ...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2012.0052

    authors: Liu J,Wang Y,Su J,Wang L,Li R,Li Q,Wu Y,Hua S,Quan F,Guo Z,Zhang Y

    更新日期:2013-04-01 00:00:00

  • Enucleation of demecolcine-treated bovine oocytes in cytochalasin-free medium: mechanism investigation and practical improvement.

    abstract::Demecolcine-assisted/induced enucleation has been used in nuclear transfer cloning procedures for many species, yet its mechanism of action remains unclear. Primarily because oocytoplasm protrusion induced by demecolcine is inhibited by the presence of cytochalasin, its use has had limited application. In this experim...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2011.0012

    authors: Meng Q,Wu X,Bunch TD,White K,Sessions BR,Davies CJ,Rickords L,Li GP

    更新日期:2011-10-01 00:00:00

  • Treatment of Donor Cells and Reconstructed Embryos with a Combination of Trichostatin-A and 5-aza-2'-Deoxycytidine Improves the Developmental Competence and Quality of Buffalo Embryos Produced by Handmade Cloning and Alters Their Epigenetic Status and Gen

    abstract::The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine (5-aza-dC), an inhibitor of DNA methyltransferases, are widely used for alteri...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2016.0061

    authors: Saini M,Selokar NL,Agrawal H,Singla SK,Chauhan MS,Manik RS,Palta P

    更新日期:2017-06-01 00:00:00

  • Cellular transitions and tissue engineering.

    abstract::Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndMT) describe complex changes in progenitor lineage, cell morphology, and gene expression. Stimulated by environmental cues, these cellular transitions are essential for elements of embryonic development and can be pathologically d...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章,评审

    doi:10.1089/cell.2012.0054

    authors: Schindeler A,Kolind M,Little DG

    更新日期:2013-04-01 00:00:00

  • The Efficacy and Safety of Sendai Viral Reprograming of Mouse Primary Cells Using Human Vectors.

    abstract::Induced pluripotent stem cells (iPSCs) remain a promising approach to target diseases with a loss of functional parenchyma. This technology comes with a number of concerns for clinical applications, including teratogenic potential and genomic instability. Here we focused on evaluating the safety of cross-species Senda...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2018.0048

    authors: Tarnawski L,Eugster E,DeCamp L,Jovinge S

    更新日期:2019-04-01 00:00:00

  • Hematopoietic Developmental Potential of Human Pluripotent Stem Cell Lines Is Accompanied by the Morphology of Embryoid Bodies and the Expression of Endodermal and Hematopoietic Markers.

    abstract::The potential clinical applications of hematopoietic stem cells (HSCs) derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid hematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available ...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2016.0042

    authors: Tesarova L,Simara P,Stejskal S,Koutna I

    更新日期:2017-08-01 00:00:00

  • Cloned sheep blastocysts derived from oocytes enucleated manually using a pulled pasteur pipette.

    abstract::The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulatio...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2012.0033

    authors: Hosseini SM,Hajian M,Moulavi F,Asgari V,Forouzanfar M,Nasr-Esfahani MH

    更新日期:2013-02-01 00:00:00

  • In Vitro Induction of Human Embryonic Stem Cells into the Midbrain Dopaminergic Neurons and Transplantation in Cynomolgus Monkey.

    abstract::A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons in vitro. It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small mol...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2019.0037

    authors: Qiu X,Liu Y,Xiao X,He J,Zhang H,Li Y

    更新日期:2019-12-01 00:00:00

  • Factors Affecting Mouse Somatic Cell Nuclear Reprogramming by Rabbit Ooplasms.

    abstract::Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells as donors...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2017.0021

    authors: Huang X,Song L,Zhan Z,Gu H,Feng H,Li Y

    更新日期:2017-12-01 00:00:00

  • Derivation of Rabbit Embryonic Stem Cells from Vitrified-Thawed Embryos.

    abstract::The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-t...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2015.0044

    authors: Du F,Chen CH,Li Y,Hu Y,An LY,Yang L,Zhang J,Chen YE,Xu J

    更新日期:2015-12-01 00:00:00

  • Generation of Induced Pluripotent Stem Cells from Bovine Epithelial Cells and Partial Redirection Toward a Mammary Phenotype In Vitro.

    abstract::In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2014.0087

    authors: Cravero D,Martignani E,Miretti S,Accornero P,Pauciullo A,Sharma R,Donadeu FX,Baratta M

    更新日期:2015-06-01 00:00:00

  • Expression of innate immune response genes in liver and three types of adipose tissue in cloned pigs.

    abstract::The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by t...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2012.0026

    authors: Rødgaard T,Skovgaard K,Stagsted J,Heegaard PM

    更新日期:2012-10-01 00:00:00

  • Transcriptome Profiling Reveals Degree of Variability in Induced Pluripotent Stem Cell Lines: Impact for Human Disease Modeling.

    abstract::Induced pluripotent stem cell (iPSC) technology has become an important tool for disease modeling. Insufficient data on the variability among iPSC lines derived from a single somatic parental cell line have in practice led to generation and analysis of several, usually three, iPSC sister lines from each parental cell ...

    journal_title:Cellular reprogramming

    pub_type: 杂志文章

    doi:10.1089/cell.2015.0009

    authors: Schuster J,Halvardson J,Pilar Lorenzo L,Ameur A,Sobol M,Raykova D,Annerén G,Feuk L,Dahl N

    更新日期:2015-10-01 00:00:00