In Vitro Induction of Human Embryonic Stem Cells into the Midbrain Dopaminergic Neurons and Transplantation in Cynomolgus Monkey.

abstract：:The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine (5-aza-dC), an inhibitor of DNA methyltransferases, are widely used for alteri...

journal_title：Cellular reprogramming

authors： Saini M,Selokar NL,Agrawal H,Singla SK,Chauhan MS,Manik RS,Palta P

更新日期：2017-06-01 00:00:00

abstract：:The generation of induced pluripotent stem cells (iPSCs) from somatic cells by expressing ectopic reprogramming transcriptional factors such as Oct3/4, Sox2, Klf4, c-Myc, and Nanog is one of the cutting-edge discoveries in stem cell and cancer research. This discovery has raised several safety issues regarding the use...

journal_title：Cellular reprogramming

authors： Ramakrishna S,Kim KS,Baek KH

更新日期：2014-04-01 00:00:00

abstract：:Male germline stem cells (mGSCs) are stem cells present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived in vitro and resemble embryonic stem cells (ESCs) properties both in the mouse and humans. However, little is know about these cells in domestic a...

journal_title：Cellular reprogramming

authors： Hua J,Zhu H,Pan S,Liu C,Sun J,Ma X,Dong W,Liu W,Li W

更新日期：2011-04-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) are generated from somatic cells through ectopic expression of defined transcription factors. So far, many iPSC lines have been established in various species, including porcine. However, the molecular events during somatic cell reprogramming in pig are largely unknown. The aim o...

journal_title：Cellular reprogramming

authors： Cheng D,Li Z,Liu Y,Gao Y,Wang H

更新日期：2012-08-01 00:00:00

abstract：:The potential clinical applications of hematopoietic stem cells (HSCs) derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid hematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available ...

journal_title：Cellular reprogramming

authors： Tesarova L,Simara P,Stejskal S,Koutna I

更新日期：2017-08-01 00:00:00

abstract：:Previous studies have shown that the time interval between fusion and activation (FA interval) play an important role in nuclear remodeling and in vitro development of somatic cell nuclear transfer (SCNT) embryos. However, the effects of FA interval on the epigenetic reprogramming and in vivo developmental competence ...

journal_title：Cellular reprogramming

authors： Liu J,Wang Y,Su J,Wang L,Li R,Li Q,Wu Y,Hua S,Quan F,Guo Z,Zhang Y

更新日期：2013-04-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) play an important role in cell replacement therapy. Several studies have shown that keratinocytes are promising reprogrammed cells. We easily and efficiently enriched epidermal stem cells by attaching them for a limited time in culture dishes. Individual epidermal cells enriched ...

journal_title：Cellular reprogramming

authors： Yang YH,Zhang RZ,Cheng S,Xu B,Tian T,Shi HX,Xiao L,Chen RH

更新日期：2018-12-01 00:00:00

abstract：:Methyl-CpG-binding domain proteins (MBPs) connect DNA methylation and histone modification, which are the key changes of somatic cell reprogramming. Methyl-CpG-binding protein 2 (MeCP2) was the first discovered MBP that has been extensively studied in the neurodevelopmental disorder Rett syndrome. However, a role for ...

journal_title：Cellular reprogramming

authors： Wang ZD,Duan L,Zhang ZH,Song SH,Bai GY,Zhang N,Shen XH,Shen JL,Lei L

更新日期：2016-04-01 00:00:00

abstract：:In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...

journal_title：Cellular reprogramming

authors： Cravero D,Martignani E,Miretti S,Accornero P,Pauciullo A,Sharma R,Donadeu FX,Baratta M

更新日期：2015-06-01 00:00:00

abstract：:Induced pluripotent stem cell (iPSC) technology has become an important tool for disease modeling. Insufficient data on the variability among iPSC lines derived from a single somatic parental cell line have in practice led to generation and analysis of several, usually three, iPSC sister lines from each parental cell ...

journal_title：Cellular reprogramming

authors： Schuster J,Halvardson J,Pilar Lorenzo L,Ameur A,Sobol M,Raykova D,Annerén G,Feuk L,Dahl N

更新日期：2015-10-01 00:00:00

abstract：:The online-ahead-of print e-pub version of the article entitled, Bone Mesenchymal Stem Cell-Conditioned Medium Regulates the Differentiation of Neural Stem Cells Via Notch Pathway Activation, by Li H-M, Tong Y, Xia X, Huang J, Song P-W, Zhang R-J, Shen C-L, utilizing the DOI number 10.1089/cell.2018.0042 is being offi...

journal_title：Cellular reprogramming

authors： Li HM,Tong Y,Xia X,Huang J,Song PW,Zhang RJ,Shen CL

更新日期：2018-12-27 00:00:00

abstract：:Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined ...

journal_title：Cellular reprogramming

authors： Peng X,Liu T,Yang B,Shi C,Sun Y,Jiang L,Jin H,Li L,Zhu H,Wu M,Qian Q

更新日期：2013-06-01 00:00:00

abstract：:Skin-derived progenitors (SKP) are neural crest derived and can generate neural and mesodermal progeny in vitro, corresponding to the multipotency of neural crest stem cells. Likewise, neural stem/progenitor cells (displaying as neurospheres) have the capacity of self-renewing, and can produce most phenotypes in the n...

journal_title：Cellular reprogramming

authors： Zhao MT,Whitworth KM,Lin H,Zhang X,Isom SC,Dobbs KB,Bauer B,Zhang Y,Prather RS

更新日期：2010-06-01 00:00:00

abstract：:The generation of human induced pluripotent stem cells (hiPSCs) derived from an autologous extraembryonic fetal source is an innovative personalized regenerative technology that can transform own-self cells into embryonic stem-like ones. These cells are regarded as a promising candidate for cell-based therapy, as well...

journal_title：Cellular reprogramming

authors： Spitalieri P,Talarico RV,Botta A,Murdocca M,D'Apice MR,Orlandi A,Giardina E,Santoro M,Brancati F,Novelli G,Sangiuolo F

更新日期：2015-08-01 00:00:00

abstract：:Adult cardiomyocytes have little ability to regenerate, thus cardiac regeneration therapy represents a potential method for treating severe heart failure. Human amniotic mesenchymal cells (hAMCs) have the potential to be a useful cell source for cardiac regeneration therapy. We attempted to isolate stem cells from hAM...

journal_title：Cellular reprogramming

authors： Otaka S,Nagura S,Koike C,Okabe M,Yoshida T,Fathy M,Yanagi K,Misaki T,Nikaido T

更新日期：2013-02-01 00:00:00

abstract：:Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated wi...

journal_title：Cellular reprogramming

authors： Whitworth KM,Mao J,Lee K,Spollen WG,Samuel MS,Walters EM,Spate LD,Prather RS

更新日期：2015-08-01 00:00:00

abstract：:Chicken embryonic stem cells (cESCs) isolated from the egg at the stage X hold great promise for cell therapy, tissue engineering, pharmaceutical, and biotechnological applications. They are considered to be pluripotent cells with the capacity to self-renewal and differentiate into specialized cells. However, long-ter...

journal_title：Cellular reprogramming

authors： Xiong C,Wang M,Ling W,Xie D,Chu X,Li Y,Huang Y,Li T,Otieno E,Qiu X,Xiao X

更新日期：2020-04-01 00:00:00

abstract：:Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT. Co-culture with f...

journal_title：Cellular reprogramming

authors： Park MJ,Lee SE,Kim EY,Lee JB,Jeong CJ,Park SP

更新日期：2015-06-01 00:00:00

abstract：:Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...

journal_title：Cellular reprogramming

authors： Wagner S,Cullum A,Wells DN,Laible G

更新日期：2017-12-01 00:00:00

abstract：:Adipose tissue-derived stromal cells (ADSCs) have been identified as a powerful stem cell source for cellular transplantation therapy. The dog is increasingly used as a model of human neurological disease; however, few studies have reported induction of canine ADSCs to neural lineages. We characterized canine ADSCs an...

journal_title：Cellular reprogramming

authors： Lim JH,Boozer L,Mariani CL,Piedrahita JA,Olby NJ

更新日期：2010-08-01 00:00:00

abstract：:Edaravone can induce differentiation of bone marrow mesenchymal stem cells (BMSCs) into neuron-like cells and replace lost cells by transplanting neuron-like cells to repair spinal cord injury (SCI). In this study, BMSCs were derived from the bone marrow of male Wistar rats (4 weeks old) through density gradient centr...

journal_title：Cellular reprogramming

authors： Li Y,Liu L,Yu Z,Yu Y,Sun B,Xiao C,Luo S,Li L

更新日期：2021-01-05 00:00:00

abstract：:The differentiation of multipotent stem cells toward a pancreatic lineage provides us with an alternative cell-based therapeutic approach to type 1 diabetes and enables us to study pancreas development. The current study aims to study the effect of growth factors such as activin A or nicotinamide, alone and in combina...

journal_title：Cellular reprogramming

authors： Balaji S,Zhou Y,Opara EC,Soker S

更新日期：2017-08-01 00:00:00

abstract：:Animal cloning by nuclear transfer (NT) has made the production of transgenic animals using genetically modified donor cells possible and ensures the presence of the gene construct in the offspring. The identification of transgene insertion sites in donor cells before cloning may avoid the production of animals that c...

journal_title：Cellular reprogramming

authors： Bressan FF,Dos Santos Miranda M,Perecin F,De Bem TH,Pereira FT,Russo-Carbolante EM,Alves D,Strauss B,Bajgelman M,Krieger JE,Binelli M,Meirelles FV

更新日期：2011-02-01 00:00:00

abstract：:Cell Stemness can be achieved by various reprogramming techniques namely, somatic cell nuclear transfer, cell fusion, cell extracts, and introduction of transcription factors from which induced pluripotent stem cells (iPSCs) are obtained. iPSCs are valuable cell sources for drug screening and human disease modeling. A...

journal_title：Cellular reprogramming

authors： Mirahmadi M,Nakhaei-Rad S,Matin MM,Shahriyari M,Saeinasab M,Mahmoudi Z,Haghighitalab A,Mahdavi-Shahri N,Bahrami AR

更新日期：2016-10-01 00:00:00

abstract：:In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as recepto...

journal_title：Cellular reprogramming

authors： Naaman H,Rabinski T,Yizhak A,Mizrahi S,Avni YS,Taube R,Rager B,Weinstein Y,Rall G,Gopas J,Ofir R

更新日期：2018-02-01 00:00:00

abstract：:Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned embryos transferred to an individual surrogate are two major factors that affect the successful rate of somatic cell nuclear transfer (SCNT) in pigs. This study aimed to compare the influence of different donor fibroblast cell type...

journal_title：Cellular reprogramming

authors： Li Z,Shi J,Liu D,Zhou R,Zeng H,Zhou X,Mai R,Zeng S,Luo L,Yu W,Zhang S,Wu Z

更新日期：2013-02-01 00:00:00

abstract：:The goals of the current study were to (1) improve culture conditions and (2) chemical passaging of bovine embryonic stem cell-like (bESC-like) cells. Specifically, the effects of human leukemia inhibitory factor (hLIF), two types of feeders, mouse embryonic fibroblast (MEF) and bovine embryonic fibroblast (BEF), as w...

journal_title：Cellular reprogramming

authors： Gong G,Roach ML,Jiang L,Yang X,Tian XC

更新日期：2010-04-01 00:00:00

abstract：:Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndMT) describe complex changes in progenitor lineage, cell morphology, and gene expression. Stimulated by environmental cues, these cellular transitions are essential for elements of embryonic development and can be pathologically d...

journal_title：Cellular reprogramming

authors： Schindeler A,Kolind M,Little DG

更新日期：2013-04-01 00:00:00

abstract：:Various diseases, injuries, and congenital abnormalities may result in degeneration and loss of organs and tissues. Recently, tissue engineering has offered new treatment options for these common, severe, and costly problems in human health care. Its application is often based on the usage of differentiated stem cells...

journal_title：Cellular reprogramming

authors： Dastagir K,Reimers K,Lazaridis A,Jahn S,Maurer V,Strauß S,Dastagir N,Radtke C,Kampmann A,Bucan V,Vogt PM

更新日期：2014-08-01 00:00:00

abstract：:Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression...

journal_title：Cellular reprogramming

authors： Wang X,Shi J,Cai G,Zheng E,Liu D,Wu Z,Li Z

更新日期：2019-10-01 00:00:00