Abstract:
:Methyl-CpG-binding domain proteins (MBPs) connect DNA methylation and histone modification, which are the key changes of somatic cell reprogramming. Methyl-CpG-binding protein 2 (MeCP2) was the first discovered MBP that has been extensively studied in the neurodevelopmental disorder Rett syndrome. However, a role for MeCP2 during cellular reprogramming associated with somatic cell nuclear transfer (SCNT) has not been examined. In this study, we discovered that MeCP2 expression was significantly lower in embryos generated by SCNT compared with those generated by intracytoplasmic sperm injection (ICSI). We genetically modified mouse embryonic fibroblasts (MEFs) to overexpress MeCP2 and serve as donor cells for nuclear transfer (NT) to investigate the effects of MeCP2 on preimplantation development of SCNT embryos. The blastocyst rate (35.71%) of MeCP2 overexpressed embryos (NT(+)) was significantly greater than in nontransgenic embryos (NT(-), 24.29%). Furthermore, immunofluorescence experiments revealed that 5-methylcytosine (5mC) was transferred to 5-hydroxymethylcytosine (5hmC) to a greater extent in NT(+) embryos than in NT(-) embryos. Real-time PCR evaluation of gene expression also showed that embryonic development-associated genes, such as Oct4 and Nanog, were significantly higher in the NT(+) group compared to the NT(-) group. Collectively, these results suggested that MeCP2 facilitated Tet3 activity, enhanced expression of pluripotency-related genes, and eventually improved the development of NT embryos. Finally, we performed chromatin immunoprecipitation to identify direct targets of MeCP2 and constructed a protein interaction network to elucidate several putative MeCP2 targets.
journal_name
Cell Reprogramjournal_title
Cellular reprogrammingauthors
Wang ZD,Duan L,Zhang ZH,Song SH,Bai GY,Zhang N,Shen XH,Shen JL,Lei Ldoi
10.1089/cell.2015.0060subject
Has Abstractpub_date
2016-04-01 00:00:00pages
78-86issue
2eissn
2152-4971issn
2152-4998journal_volume
18pub_type
杂志文章abstract::The generation of induced pluripotent stem cells (iPSCs) from somatic cells by expressing ectopic reprogramming transcriptional factors such as Oct3/4, Sox2, Klf4, c-Myc, and Nanog is one of the cutting-edge discoveries in stem cell and cancer research. This discovery has raised several safety issues regarding the use...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0077
更新日期:2014-04-01 00:00:00
abstract::Animal cloning by nuclear transfer (NT) has made the production of transgenic animals using genetically modified donor cells possible and ensures the presence of the gene construct in the offspring. The identification of transgene insertion sites in donor cells before cloning may avoid the production of animals that c...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0022
更新日期:2011-02-01 00:00:00
abstract::Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0065
更新日期:2013-06-01 00:00:00
abstract::Although numerous mammalian species have been successfully cloned by somatic cell nuclear transfer (SCNT), little is known about gene expression of cloned pigs by SCNT. In the present study, expression profiles of 1-month-old cloned pigs generated from fetal fibroblasts (n = 5) were compared to those of age-matched co...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0088
更新日期:2011-06-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0049
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abstract::Male germline stem cells (mGSCs) are stem cells present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived in vitro and resemble embryonic stem cells (ESCs) properties both in the mouse and humans. However, little is know about these cells in domestic a...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0047
更新日期:2011-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0034
更新日期:2018-02-01 00:00:00
abstract::Buffalo embryos were produced by hand-made cloning using skin fibroblasts from male and female buffaloes (n = 4 each) as donor cells for examining the effect of sex. Although the rate of blastocyst formation (43.8% ± 1.31% vs. 42.2% ± 1.22%) was similar, the total cell number (333 ± 10.4 vs. 270 ± 10.9) was higher (p ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0077
更新日期:2016-10-01 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndMT) describe complex changes in progenitor lineage, cell morphology, and gene expression. Stimulated by environmental cues, these cellular transitions are essential for elements of embryonic development and can be pathologically d...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2012.0054
更新日期:2013-04-01 00:00:00
abstract::Based on microarray data comparing gene expression of fibroblast donor cells and bovine somatic cell nuclear transfer (SCNT) and in vivo produced (AI) blastocysts, a group of genes including several transcription factors was selected for evaluation of transcript abundance. Using SYBR green-based real-time polymerase c...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0042
更新日期:2010-02-01 00:00:00
abstract::In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0087
更新日期:2015-06-01 00:00:00
abstract::The generation of human induced pluripotent stem cells (hiPSCs) derived from an autologous extraembryonic fetal source is an innovative personalized regenerative technology that can transform own-self cells into embryonic stem-like ones. These cells are regarded as a promising candidate for cell-based therapy, as well...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0003
更新日期:2015-08-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) remain a promising approach to target diseases with a loss of functional parenchyma. This technology comes with a number of concerns for clinical applications, including teratogenic potential and genomic instability. Here we focused on evaluating the safety of cross-species Senda...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2018.0048
更新日期:2019-04-01 00:00:00
abstract::As man's best friend, dogs have an important position in human society. Ten years ago, we reported the first cloned dog, and his birth has raised various scientific issues, such as those related to health, reproduction, and life span. He has developed without any unique health issues. In this article, we summarize and...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0033
更新日期:2015-10-01 00:00:00
abstract::The potential clinical applications of hematopoietic stem cells (HSCs) derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid hematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0042
更新日期:2017-08-01 00:00:00
abstract::Previous studies have shown that the time interval between fusion and activation (FA interval) play an important role in nuclear remodeling and in vitro development of somatic cell nuclear transfer (SCNT) embryos. However, the effects of FA interval on the epigenetic reprogramming and in vivo developmental competence ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0052
更新日期:2013-04-01 00:00:00
abstract::The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-t...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0044
更新日期:2015-12-01 00:00:00
abstract::The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by t...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0026
更新日期:2012-10-01 00:00:00
abstract::Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned embryos transferred to an individual surrogate are two major factors that affect the successful rate of somatic cell nuclear transfer (SCNT) in pigs. This study aimed to compare the influence of different donor fibroblast cell type...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0042
更新日期:2013-02-01 00:00:00
abstract::Chicken embryonic stem cells (cESCs) isolated from the egg at the stage X hold great promise for cell therapy, tissue engineering, pharmaceutical, and biotechnological applications. They are considered to be pluripotent cells with the capacity to self-renewal and differentiate into specialized cells. However, long-ter...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0080
更新日期:2020-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2011.0067
更新日期:2012-04-01 00:00:00
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journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0089
更新日期:2011-08-01 00:00:00
abstract::The purpose of this study was to investigate the effects of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on transgene expression and development of porcine transgenic cloned embryos, specifically focusing on effects derived from TSA-treated donor cells or TSA-treated reconstructed embryos. The results...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0041
更新日期:2015-02-01 00:00:00
abstract::The present study compared the potential of porcine bone marrow mesenchymal stem cells (pBMSCs) at different passages as nuclear transfer (NT) donors and the developmental efficiency of NT embryos from donor cells transfected with/without Oct4 and Sox2. Early-passage pBMSCs showed higher proliferation and expression o...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0036
更新日期:2014-12-01 00:00:00
abstract::The scope of this article is not to provide an exhaustive review of nuclear transfer research, because many authoritative reviews exist on the biological issues related to somatic and embryonic cell nuclear transfer. We shall instead provide an overview on the work done specifically on sheep and the value of this work...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0032
更新日期:2013-10-01 00:00:00
abstract::The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine (5-aza-dC), an inhibitor of DNA methyltransferases, are widely used for alteri...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0061
更新日期:2017-06-01 00:00:00
abstract::Edaravone can induce differentiation of bone marrow mesenchymal stem cells (BMSCs) into neuron-like cells and replace lost cells by transplanting neuron-like cells to repair spinal cord injury (SCI). In this study, BMSCs were derived from the bone marrow of male Wistar rats (4 weeks old) through density gradient centr...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2020.0055
更新日期:2021-01-05 00:00:00
abstract::The online-ahead-of print e-pub version of the article entitled, Bone Mesenchymal Stem Cell-Conditioned Medium Regulates the Differentiation of Neural Stem Cells Via Notch Pathway Activation, by Li H-M, Tong Y, Xia X, Huang J, Song P-W, Zhang R-J, Shen C-L, utilizing the DOI number 10.1089/cell.2018.0042 is being offi...
journal_title:Cellular reprogramming
pub_type: 杂志文章,收录出版
doi:10.1089/cell.2018.0042
更新日期:2018-12-27 00:00:00
abstract::Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0008
更新日期:2019-10-01 00:00:00
abstract::Chickens are vitally important in numerous countries as a primary food source and a major component of economic development. Efforts have been made to produce transgenic birds through pluripotent stem cell [primordial germ cells and embryonic stem cells (ESCs)] approaches to create animals with improved traits, such a...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0028
更新日期:2014-10-01 00:00:00