Overexpression of MBD3 Improves Reprogramming of Cloned Pig Embryos.

Abstract:

:Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression level of MBD3 was demonstrated to be higher in somatic cell nuclear transfer-generated cloned pig embryos than in fertilization-derived porcine embryos. However, the functions of MBD3 in nuclear transfer-mediated somatic cell reprogramming are unknown. In this study, MBD3 was overexpressed in cloned pig embryos, and the effects of MBD3 overexpression on gene transcription, DNA methylation, and in vitro developmental competence of cloned pig embryos were analyzed. Results indicated that overexpression of MBD3 in cloned pig embryos not only increased blastocyst rate and number of cells per blastocyst but also upregulated mRNA expression levels and decreased the DNA methylation of NANOG, OCT4, and LINE1 genes to the levels close to those in in vivo fertilization-produced pig embryos. These findings suggest that overexpression of MBD3 improves reprogramming of cloned pig embryos.

journal_name

Cell Reprogram

journal_title

Cellular reprogramming

authors

Wang X,Shi J,Cai G,Zheng E,Liu D,Wu Z,Li Z

doi

10.1089/cell.2019.0008

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

221-228

issue

5

eissn

2152-4971

issn

2152-4998

journal_volume

21

pub_type

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