Abstract:
:Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndMT) describe complex changes in progenitor lineage, cell morphology, and gene expression. Stimulated by environmental cues, these cellular transitions are essential for elements of embryonic development and can be pathologically dysregulated in disease states. EMT occurs in biological processes such as gastrulation, cardiogenesis, and fibrosis. EndMT is involved in development and tissue fibrosis, but recent studies have implicated this process in musculoskeletal biology and pathology. Tissue engineering and regenerative medicine typically rely on endogenous progenitors or progenitors expanded ex vivo to repair damaged or impaired tissues or organs. The processes of EMT and EndMT may aid in elucidating new methods for reducing fibrosis and identifying novel plastic progenitor populations for tissue repair. This review will discuss the potential for EMT and EndMT to impact on tissue engineering and regenerative medicine.
journal_name
Cell Reprogramjournal_title
Cellular reprogrammingauthors
Schindeler A,Kolind M,Little DGdoi
10.1089/cell.2012.0054subject
Has Abstractpub_date
2013-04-01 00:00:00pages
101-6issue
2eissn
2152-4971issn
2152-4998journal_volume
15pub_type
杂志文章,评审abstract::Demecolcine-assisted/induced enucleation has been used in nuclear transfer cloning procedures for many species, yet its mechanism of action remains unclear. Primarily because oocytoplasm protrusion induced by demecolcine is inhibited by the presence of cytochalasin, its use has had limited application. In this experim...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2011.0012
更新日期:2011-10-01 00:00:00
abstract::Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0008
更新日期:2019-10-01 00:00:00
abstract::The online-ahead-of print e-pub version of the article entitled, Bone Mesenchymal Stem Cell-Conditioned Medium Regulates the Differentiation of Neural Stem Cells Via Notch Pathway Activation, by Li H-M, Tong Y, Xia X, Huang J, Song P-W, Zhang R-J, Shen C-L, utilizing the DOI number 10.1089/cell.2018.0042 is being offi...
journal_title:Cellular reprogramming
pub_type: 杂志文章,收录出版
doi:10.1089/cell.2018.0042
更新日期:2018-12-27 00:00:00
abstract::Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0018
更新日期:2017-12-01 00:00:00
abstract::Based on microarray data comparing gene expression of fibroblast donor cells and bovine somatic cell nuclear transfer (SCNT) and in vivo produced (AI) blastocysts, a group of genes including several transcription factors was selected for evaluation of transcript abundance. Using SYBR green-based real-time polymerase c...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0042
更新日期:2010-02-01 00:00:00
abstract::Animal cloning by nuclear transfer (NT) has made the production of transgenic animals using genetically modified donor cells possible and ensures the presence of the gene construct in the offspring. The identification of transgene insertion sites in donor cells before cloning may avoid the production of animals that c...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0022
更新日期:2011-02-01 00:00:00
abstract::Induced pluripotent stem cell (iPSC) technology has become an important tool for disease modeling. Insufficient data on the variability among iPSC lines derived from a single somatic parental cell line have in practice led to generation and analysis of several, usually three, iPSC sister lines from each parental cell ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0009
更新日期:2015-10-01 00:00:00
abstract::Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells as donors...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0021
更新日期:2017-12-01 00:00:00
abstract::The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by t...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0026
更新日期:2012-10-01 00:00:00
abstract::Previous studies have shown that the time interval between fusion and activation (FA interval) play an important role in nuclear remodeling and in vitro development of somatic cell nuclear transfer (SCNT) embryos. However, the effects of FA interval on the epigenetic reprogramming and in vivo developmental competence ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0052
更新日期:2013-04-01 00:00:00
abstract::As man's best friend, dogs have an important position in human society. Ten years ago, we reported the first cloned dog, and his birth has raised various scientific issues, such as those related to health, reproduction, and life span. He has developed without any unique health issues. In this article, we summarize and...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0033
更新日期:2015-10-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) are generated from somatic cells through ectopic expression of defined transcription factors. So far, many iPSC lines have been established in various species, including porcine. However, the molecular events during somatic cell reprogramming in pig are largely unknown. The aim o...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0025
更新日期:2012-08-01 00:00:00
abstract::Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lin...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0083
更新日期:2020-04-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) remain a promising approach to target diseases with a loss of functional parenchyma. This technology comes with a number of concerns for clinical applications, including teratogenic potential and genomic instability. Here we focused on evaluating the safety of cross-species Senda...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2018.0048
更新日期:2019-04-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this species. But such tools do not yet exist for many rat strains, especially for di...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0028
更新日期:2017-06-01 00:00:00
abstract::The scope of this article is not to provide an exhaustive review of nuclear transfer research, because many authoritative reviews exist on the biological issues related to somatic and embryonic cell nuclear transfer. We shall instead provide an overview on the work done specifically on sheep and the value of this work...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0032
更新日期:2013-10-01 00:00:00
abstract::Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0065
更新日期:2013-06-01 00:00:00
abstract::In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0087
更新日期:2015-06-01 00:00:00
abstract::Transgenic animal producing technology has improved consistently over the last couple of decades. Among the available methods, somatic cell nuclear transfer (SCNT) technology was officially the most popular. However, SCNT has low efficiency and requires a highly skilled individual. Additionally, the allo-SCNT nuclear ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0065
更新日期:2016-08-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) play an important role in cell replacement therapy. Several studies have shown that keratinocytes are promising reprogrammed cells. We easily and efficiently enriched epidermal stem cells by attaching them for a limited time in culture dishes. Individual epidermal cells enriched ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2018.0035
更新日期:2018-12-01 00:00:00
abstract::Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of the donor cell. Previous studies suggest cellular heterogeneity among donor nuclei in regard to reprogramming potential, which precludes comparison among different strategies to increase cloning success...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0063
更新日期:2017-10-01 00:00:00
abstract::A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons in vitro. It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small mol...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0037
更新日期:2019-12-01 00:00:00
abstract::The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine (5-aza-dC), an inhibitor of DNA methyltransferases, are widely used for alteri...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0061
更新日期:2017-06-01 00:00:00
abstract::The goals of the current study were to (1) improve culture conditions and (2) chemical passaging of bovine embryonic stem cell-like (bESC-like) cells. Specifically, the effects of human leukemia inhibitory factor (hLIF), two types of feeders, mouse embryonic fibroblast (MEF) and bovine embryonic fibroblast (BEF), as w...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0049
更新日期:2010-04-01 00:00:00
abstract::Chickens are vitally important in numerous countries as a primary food source and a major component of economic development. Efforts have been made to produce transgenic birds through pluripotent stem cell [primordial germ cells and embryonic stem cells (ESCs)] approaches to create animals with improved traits, such a...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0028
更新日期:2014-10-01 00:00:00
abstract::The potential clinical applications of hematopoietic stem cells (HSCs) derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid hematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0042
更新日期:2017-08-01 00:00:00
abstract::The generation of defined somatic cell types from pluripotent stem cells represents a promising system for many applications for regenerative therapy or developmental studies. Certain key developmental genes have been shown to be able to influence the fate determination of differentiating stem cells suggesting an alte...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2011.0067
更新日期:2012-04-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) are promising candidates for the study of disease models as well as for tissue engineering purposes. Part of a strategy to develop safe reprogramming technique is reducing the number of exogenous reprogramming factors. Some cells types are more prone to reprogramming than others....
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0018
更新日期:2014-10-01 00:00:00
abstract::Recent work has shown that mouse and human fibroblasts can be reprogrammed to cardiomyocyte-like cells with a combination of transcription factors. Current research has focused on improving the efficiency and mechanisms for fibroblast reprogramming. Previously, it has been reported that hypoxia enhances fibroblast cel...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0051
更新日期:2016-02-01 00:00:00
abstract::The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-t...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0044
更新日期:2015-12-01 00:00:00