Altered gene expression profiles in the brain, kidney, and lung of one-month-old cloned pigs.

abstract：:Induced pluripotent stem cell (iPSC) technology has become an important tool for disease modeling. Insufficient data on the variability among iPSC lines derived from a single somatic parental cell line have in practice led to generation and analysis of several, usually three, iPSC sister lines from each parental cell ...

journal_title：Cellular reprogramming

authors： Schuster J,Halvardson J,Pilar Lorenzo L,Ameur A,Sobol M,Raykova D,Annerén G,Feuk L,Dahl N

更新日期：2015-10-01 00:00:00

abstract：:Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndMT) describe complex changes in progenitor lineage, cell morphology, and gene expression. Stimulated by environmental cues, these cellular transitions are essential for elements of embryonic development and can be pathologically d...

journal_title：Cellular reprogramming

authors： Schindeler A,Kolind M,Little DG

更新日期：2013-04-01 00:00:00

abstract：:Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined ...

journal_title：Cellular reprogramming

authors： Peng X,Liu T,Yang B,Shi C,Sun Y,Jiang L,Jin H,Li L,Zhu H,Wu M,Qian Q

更新日期：2013-06-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) play an important role in cell replacement therapy. Several studies have shown that keratinocytes are promising reprogrammed cells. We easily and efficiently enriched epidermal stem cells by attaching them for a limited time in culture dishes. Individual epidermal cells enriched ...

journal_title：Cellular reprogramming

authors： Yang YH,Zhang RZ,Cheng S,Xu B,Tian T,Shi HX,Xiao L,Chen RH

更新日期：2018-12-01 00:00:00

abstract：:Abstract Professor Keith Campbell's critical contribution to the discovery that a somatic cell from an adult animal can be fully reprogrammed by oocyte factors to form a cloned individual following nuclear transfer (NT)(Wilmut et al., 1997 ) overturned a dogma concerning the reversibility of cell fate that many scient...

journal_title：Cellular reprogramming

authors： Wells DN

更新日期：2013-10-01 00:00:00

abstract：:As man's best friend, dogs have an important position in human society. Ten years ago, we reported the first cloned dog, and his birth has raised various scientific issues, such as those related to health, reproduction, and life span. He has developed without any unique health issues. In this article, we summarize and...

journal_title：Cellular reprogramming

authors： Jang G,Lee B

更新日期：2015-10-01 00:00:00

abstract：:Methyl-CpG-binding domain proteins (MBPs) connect DNA methylation and histone modification, which are the key changes of somatic cell reprogramming. Methyl-CpG-binding protein 2 (MeCP2) was the first discovered MBP that has been extensively studied in the neurodevelopmental disorder Rett syndrome. However, a role for ...

journal_title：Cellular reprogramming

authors： Wang ZD,Duan L,Zhang ZH,Song SH,Bai GY,Zhang N,Shen XH,Shen JL,Lei L

更新日期：2016-04-01 00:00:00

abstract：:The purpose of this study was to investigate the effects of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on transgene expression and development of porcine transgenic cloned embryos, specifically focusing on effects derived from TSA-treated donor cells or TSA-treated reconstructed embryos. The results...

journal_title：Cellular reprogramming

authors： Luo B,Ju S,Muneri CW,Rui R

更新日期：2015-02-01 00:00:00

abstract：:Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells as donors...

journal_title：Cellular reprogramming

authors： Huang X,Song L,Zhan Z,Gu H,Feng H,Li Y

更新日期：2017-12-01 00:00:00

abstract：:Male germline stem cells (mGSCs) are stem cells present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived in vitro and resemble embryonic stem cells (ESCs) properties both in the mouse and humans. However, little is know about these cells in domestic a...

journal_title：Cellular reprogramming

authors： Hua J,Zhu H,Pan S,Liu C,Sun J,Ma X,Dong W,Liu W,Li W

更新日期：2011-04-01 00:00:00

abstract：:Based on microarray data comparing gene expression of fibroblast donor cells and bovine somatic cell nuclear transfer (SCNT) and in vivo produced (AI) blastocysts, a group of genes including several transcription factors was selected for evaluation of transcript abundance. Using SYBR green-based real-time polymerase c...

journal_title：Cellular reprogramming

authors： Aston KI,Li GP,Hicks BA,Sessions BR,Davis AP,Rickords LF,Stevens JR,White KL

更新日期：2010-02-01 00:00:00

abstract：:Chicken embryonic stem cells (cESCs) isolated from the egg at the stage X hold great promise for cell therapy, tissue engineering, pharmaceutical, and biotechnological applications. They are considered to be pluripotent cells with the capacity to self-renewal and differentiate into specialized cells. However, long-ter...

journal_title：Cellular reprogramming

authors： Xiong C,Wang M,Ling W,Xie D,Chu X,Li Y,Huang Y,Li T,Otieno E,Qiu X,Xiao X

更新日期：2020-04-01 00:00:00

abstract：:Adipose tissue-derived stromal cells (ADSCs) have been identified as a powerful stem cell source for cellular transplantation therapy. The dog is increasingly used as a model of human neurological disease; however, few studies have reported induction of canine ADSCs to neural lineages. We characterized canine ADSCs an...

journal_title：Cellular reprogramming

authors： Lim JH,Boozer L,Mariani CL,Piedrahita JA,Olby NJ

更新日期：2010-08-01 00:00:00

abstract：:The generation of induced pluripotent stem cells (iPSCs) from somatic cells by expressing ectopic reprogramming transcriptional factors such as Oct3/4, Sox2, Klf4, c-Myc, and Nanog is one of the cutting-edge discoveries in stem cell and cancer research. This discovery has raised several safety issues regarding the use...

journal_title：Cellular reprogramming

authors： Ramakrishna S,Kim KS,Baek KH

更新日期：2014-04-01 00:00:00

abstract：:Transgenic animal producing technology has improved consistently over the last couple of decades. Among the available methods, somatic cell nuclear transfer (SCNT) technology was officially the most popular. However, SCNT has low efficiency and requires a highly skilled individual. Additionally, the allo-SCNT nuclear ...

journal_title：Cellular reprogramming

authors： Hwang JH,Kim SE,Gupta MK,Lee H

更新日期：2016-08-01 00:00:00

abstract：:The scope of this article is not to provide an exhaustive review of nuclear transfer research, because many authoritative reviews exist on the biological issues related to somatic and embryonic cell nuclear transfer. We shall instead provide an overview on the work done specifically on sheep and the value of this work...

journal_title：Cellular reprogramming

authors： Loi P,Czernik M,Zacchini F,Iuso D,Scapolo PA,Ptak G

更新日期：2013-10-01 00:00:00

abstract：:Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optimize the survival and cryopreservation of VT oocytes for SCNT. Co-culture with f...

journal_title：Cellular reprogramming

authors： Park MJ,Lee SE,Kim EY,Lee JB,Jeong CJ,Park SP

更新日期：2015-06-01 00:00:00

abstract：:Edaravone can induce differentiation of bone marrow mesenchymal stem cells (BMSCs) into neuron-like cells and replace lost cells by transplanting neuron-like cells to repair spinal cord injury (SCI). In this study, BMSCs were derived from the bone marrow of male Wistar rats (4 weeks old) through density gradient centr...

journal_title：Cellular reprogramming

authors： Li Y,Liu L,Yu Z,Yu Y,Sun B,Xiao C,Luo S,Li L

更新日期：2021-01-05 00:00:00

abstract：:Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression...

journal_title：Cellular reprogramming

authors： Wang X,Shi J,Cai G,Zheng E,Liu D,Wu Z,Li Z

更新日期：2019-10-01 00:00:00

abstract：:Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of ...

journal_title：Cellular reprogramming

authors： Sekhavati MH,Shadanloo F,Hosseini MS,Tahmoorespur M,Nasiri MR,Hajian M,Nasr-Esfahani MH

更新日期：2012-08-01 00:00:00

abstract：:Demecolcine-assisted/induced enucleation has been used in nuclear transfer cloning procedures for many species, yet its mechanism of action remains unclear. Primarily because oocytoplasm protrusion induced by demecolcine is inhibited by the presence of cytochalasin, its use has had limited application. In this experim...

journal_title：Cellular reprogramming

authors： Meng Q,Wu X,Bunch TD,White K,Sessions BR,Davies CJ,Rickords L,Li GP

更新日期：2011-10-01 00:00:00

abstract：:Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of the donor cell. Previous studies suggest cellular heterogeneity among donor nuclei in regard to reprogramming potential, which precludes comparison among different strategies to increase cloning success...

journal_title：Cellular reprogramming

authors： Sá AL,Sampaio RV,da Costa Almeida NN,Sangalli JR,Brito KNL,Bressan FF,Rissino JD,do Socorro Damasceno Santos S,Meirelles FV,Ohashi OM,Dos Santos Miranda M

更新日期：2017-10-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) remain a promising approach to target diseases with a loss of functional parenchyma. This technology comes with a number of concerns for clinical applications, including teratogenic potential and genomic instability. Here we focused on evaluating the safety of cross-species Senda...

journal_title：Cellular reprogramming

authors： Tarnawski L,Eugster E,DeCamp L,Jovinge S

更新日期：2019-04-01 00:00:00

abstract：:A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons in vitro. It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small mol...

journal_title：Cellular reprogramming

authors： Qiu X,Liu Y,Xiao X,He J,Zhang H,Li Y

更新日期：2019-12-01 00:00:00

abstract：:In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as recepto...

journal_title：Cellular reprogramming

authors： Naaman H,Rabinski T,Yizhak A,Mizrahi S,Avni YS,Taube R,Rager B,Weinstein Y,Rall G,Gopas J,Ofir R

更新日期：2018-02-01 00:00:00

abstract：:Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated wi...

journal_title：Cellular reprogramming

authors： Whitworth KM,Mao J,Lee K,Spollen WG,Samuel MS,Walters EM,Spate LD,Prather RS

更新日期：2015-08-01 00:00:00

abstract：:The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine (5-aza-dC), an inhibitor of DNA methyltransferases, are widely used for alteri...

journal_title：Cellular reprogramming

authors： Saini M,Selokar NL,Agrawal H,Singla SK,Chauhan MS,Manik RS,Palta P

更新日期：2017-06-01 00:00:00

abstract：:Recent work has shown that mouse and human fibroblasts can be reprogrammed to cardiomyocyte-like cells with a combination of transcription factors. Current research has focused on improving the efficiency and mechanisms for fibroblast reprogramming. Previously, it has been reported that hypoxia enhances fibroblast cel...

journal_title：Cellular reprogramming

authors： Wang Y,Shi S,Liu H,Meng L

更新日期：2016-02-01 00:00:00

abstract：:Chickens are vitally important in numerous countries as a primary food source and a major component of economic development. Efforts have been made to produce transgenic birds through pluripotent stem cell [primordial germ cells and embryonic stem cells (ESCs)] approaches to create animals with improved traits, such a...

journal_title：Cellular reprogramming

authors： Yu P,Lu Y,Jordan BJ,Liu Y,Yang JY,Hutcheson JM,Ethridge CL,Mumaw JL,Kinder HA,Beckstead RB,Stice SL,West FD

更新日期：2014-10-01 00:00:00

abstract：:Various diseases, injuries, and congenital abnormalities may result in degeneration and loss of organs and tissues. Recently, tissue engineering has offered new treatment options for these common, severe, and costly problems in human health care. Its application is often based on the usage of differentiated stem cells...

journal_title：Cellular reprogramming

authors： Dastagir K,Reimers K,Lazaridis A,Jahn S,Maurer V,Strauß S,Dastagir N,Radtke C,Kampmann A,Bucan V,Vogt PM

更新日期：2014-08-01 00:00:00