Abstract:
:Recent work has shown that mouse and human fibroblasts can be reprogrammed to cardiomyocyte-like cells with a combination of transcription factors. Current research has focused on improving the efficiency and mechanisms for fibroblast reprogramming. Previously, it has been reported that hypoxia enhances fibroblast cell reprogramming to pluripotent stem cells. In this study, we observed that 6 h of hypoxic conditions (2% oxygen) on newborn mouse dermal fibroblasts can improve the efficiency of reprogramming to cardiomyocyte-like cells. Expression of cardiac-related genes and proteins increased at 4 weeks after transfer of three transcription factors (Gata4/Mef2c/Tbx5 [GMT]). However, beating cardiomyocyte cells were not detected. The epigenetic mechanism of hypoxia-induced fibroblast reprogramming to cardiomyocyte cells requires further study.
journal_name
Cell Reprogramjournal_title
Cellular reprogrammingauthors
Wang Y,Shi S,Liu H,Meng Ldoi
10.1089/cell.2015.0051subject
Has Abstractpub_date
2016-02-01 00:00:00pages
1-7issue
1eissn
2152-4971issn
2152-4998journal_volume
18pub_type
杂志文章abstract::The generation of induced pluripotent stem cells (iPSCs) from somatic cells by expressing ectopic reprogramming transcriptional factors such as Oct3/4, Sox2, Klf4, c-Myc, and Nanog is one of the cutting-edge discoveries in stem cell and cancer research. This discovery has raised several safety issues regarding the use...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0077
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abstract::Male germline stem cells (mGSCs) are stem cells present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived in vitro and resemble embryonic stem cells (ESCs) properties both in the mouse and humans. However, little is know about these cells in domestic a...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0047
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abstract::The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-t...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0044
更新日期:2015-12-01 00:00:00
abstract::Cell Stemness can be achieved by various reprogramming techniques namely, somatic cell nuclear transfer, cell fusion, cell extracts, and introduction of transcription factors from which induced pluripotent stem cells (iPSCs) are obtained. iPSCs are valuable cell sources for drug screening and human disease modeling. A...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0008
更新日期:2016-10-01 00:00:00
abstract::Based on microarray data comparing gene expression of fibroblast donor cells and bovine somatic cell nuclear transfer (SCNT) and in vivo produced (AI) blastocysts, a group of genes including several transcription factors was selected for evaluation of transcript abundance. Using SYBR green-based real-time polymerase c...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0042
更新日期:2010-02-01 00:00:00
abstract::Animal cloning by nuclear transfer (NT) has made the production of transgenic animals using genetically modified donor cells possible and ensures the presence of the gene construct in the offspring. The identification of transgene insertion sites in donor cells before cloning may avoid the production of animals that c...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0022
更新日期:2011-02-01 00:00:00
abstract::Abstract Professor Keith Campbell's critical contribution to the discovery that a somatic cell from an adult animal can be fully reprogrammed by oocyte factors to form a cloned individual following nuclear transfer (NT)(Wilmut et al., 1997 ) overturned a dogma concerning the reversibility of cell fate that many scient...
journal_title:Cellular reprogramming
pub_type: 传,历史文章,杂志文章
doi:10.1089/cell.2013.0038
更新日期:2013-10-01 00:00:00
abstract::Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated wi...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0022
更新日期:2015-08-01 00:00:00
abstract::Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned embryos transferred to an individual surrogate are two major factors that affect the successful rate of somatic cell nuclear transfer (SCNT) in pigs. This study aimed to compare the influence of different donor fibroblast cell type...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0042
更新日期:2013-02-01 00:00:00
abstract::Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2017.0018
更新日期:2017-12-01 00:00:00
abstract::The differentiation of multipotent stem cells toward a pancreatic lineage provides us with an alternative cell-based therapeutic approach to type 1 diabetes and enables us to study pancreas development. The current study aims to study the effect of growth factors such as activin A or nicotinamide, alone and in combina...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0043
更新日期:2017-08-01 00:00:00
abstract::Methyl-CpG-binding domain protein 3 (MBD3) is a core component of the nucleosome remodeling and deacetylase (NuRD) complex, which is crucial for pluripotent stem cell differentiation and embryonic development. MBD3 was shown to play important roles in transcription factor-induced somatic cell reprogramming. Expression...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0008
更新日期:2019-10-01 00:00:00
abstract::Chickens are vitally important in numerous countries as a primary food source and a major component of economic development. Efforts have been made to produce transgenic birds through pluripotent stem cell [primordial germ cells and embryonic stem cells (ESCs)] approaches to create animals with improved traits, such a...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0028
更新日期:2014-10-01 00:00:00
abstract::A simple, rapid, efficient, and specialized culture system was successfully developed in this study to induce human embryonic stem cells into dopaminergic neurons in vitro. It only took 5 days to generate quickly and directly a large number of homogeneous neural stem cell (NSC) spheres by the introduction of small mol...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0037
更新日期:2019-12-01 00:00:00
abstract::The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulatio...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0033
更新日期:2013-02-01 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndMT) describe complex changes in progenitor lineage, cell morphology, and gene expression. Stimulated by environmental cues, these cellular transitions are essential for elements of embryonic development and can be pathologically d...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2012.0054
更新日期:2013-04-01 00:00:00
abstract::Adipose tissue-derived stromal cells (ADSCs) have been identified as a powerful stem cell source for cellular transplantation therapy. The dog is increasingly used as a model of human neurological disease; however, few studies have reported induction of canine ADSCs to neural lineages. We characterized canine ADSCs an...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2009.0093
更新日期:2010-08-01 00:00:00
abstract::Adult cardiomyocytes have little ability to regenerate, thus cardiac regeneration therapy represents a potential method for treating severe heart failure. Human amniotic mesenchymal cells (hAMCs) have the potential to be a useful cell source for cardiac regeneration therapy. We attempted to isolate stem cells from hAM...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0028
更新日期:2013-02-01 00:00:00
abstract::The present study compared the potential of porcine bone marrow mesenchymal stem cells (pBMSCs) at different passages as nuclear transfer (NT) donors and the developmental efficiency of NT embryos from donor cells transfected with/without Oct4 and Sox2. Early-passage pBMSCs showed higher proliferation and expression o...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0036
更新日期:2014-12-01 00:00:00
abstract::Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0065
更新日期:2013-06-01 00:00:00
abstract::Previous studies have shown that the time interval between fusion and activation (FA interval) play an important role in nuclear remodeling and in vitro development of somatic cell nuclear transfer (SCNT) embryos. However, the effects of FA interval on the epigenetic reprogramming and in vivo developmental competence ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0052
更新日期:2013-04-01 00:00:00
abstract::Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lin...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2019.0083
更新日期:2020-04-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) play an important role in cell replacement therapy. Several studies have shown that keratinocytes are promising reprogrammed cells. We easily and efficiently enriched epidermal stem cells by attaching them for a limited time in culture dishes. Individual epidermal cells enriched ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2018.0035
更新日期:2018-12-01 00:00:00
abstract::Although numerous mammalian species have been successfully cloned by somatic cell nuclear transfer (SCNT), little is known about gene expression of cloned pigs by SCNT. In the present study, expression profiles of 1-month-old cloned pigs generated from fetal fibroblasts (n = 5) were compared to those of age-matched co...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2010.0088
更新日期:2011-06-01 00:00:00
abstract::Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0014
更新日期:2012-08-01 00:00:00
abstract::The scope of this article is not to provide an exhaustive review of nuclear transfer research, because many authoritative reviews exist on the biological issues related to somatic and embryonic cell nuclear transfer. We shall instead provide an overview on the work done specifically on sheep and the value of this work...
journal_title:Cellular reprogramming
pub_type: 杂志文章,评审
doi:10.1089/cell.2013.0032
更新日期:2013-10-01 00:00:00
abstract::Induced pluripotent stem cell (iPSC) technology has become an important tool for disease modeling. Insufficient data on the variability among iPSC lines derived from a single somatic parental cell line have in practice led to generation and analysis of several, usually three, iPSC sister lines from each parental cell ...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2015.0009
更新日期:2015-10-01 00:00:00
abstract::The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression of innate immune factors and that their expression would remain unaffected by t...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2012.0026
更新日期:2012-10-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this species. But such tools do not yet exist for many rat strains, especially for di...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2016.0028
更新日期:2017-06-01 00:00:00
abstract::Various diseases, injuries, and congenital abnormalities may result in degeneration and loss of organs and tissues. Recently, tissue engineering has offered new treatment options for these common, severe, and costly problems in human health care. Its application is often based on the usage of differentiated stem cells...
journal_title:Cellular reprogramming
pub_type: 杂志文章
doi:10.1089/cell.2014.0005
更新日期:2014-08-01 00:00:00