Acclimatized induction reveals the multipotency of adult human undifferentiated keratinocytes.

abstract：:Animal cloning by nuclear transfer (NT) has made the production of transgenic animals using genetically modified donor cells possible and ensures the presence of the gene construct in the offspring. The identification of transgene insertion sites in donor cells before cloning may avoid the production of animals that c...

journal_title：Cellular reprogramming

authors： Bressan FF,Dos Santos Miranda M,Perecin F,De Bem TH,Pereira FT,Russo-Carbolante EM,Alves D,Strauss B,Bajgelman M,Krieger JE,Binelli M,Meirelles FV

更新日期：2011-02-01 00:00:00

abstract：:The generation of induced pluripotent stem cells (iPSCs) from somatic cells by expressing ectopic reprogramming transcriptional factors such as Oct3/4, Sox2, Klf4, c-Myc, and Nanog is one of the cutting-edge discoveries in stem cell and cancer research. This discovery has raised several safety issues regarding the use...

journal_title：Cellular reprogramming

authors： Ramakrishna S,Kim KS,Baek KH

更新日期：2014-04-01 00:00:00

abstract：:Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of the donor cell. Previous studies suggest cellular heterogeneity among donor nuclei in regard to reprogramming potential, which precludes comparison among different strategies to increase cloning success...

journal_title：Cellular reprogramming

authors： Sá AL,Sampaio RV,da Costa Almeida NN,Sangalli JR,Brito KNL,Bressan FF,Rissino JD,do Socorro Damasceno Santos S,Meirelles FV,Ohashi OM,Dos Santos Miranda M

更新日期：2017-10-01 00:00:00

abstract：:The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-t...

journal_title：Cellular reprogramming

authors： Du F,Chen CH,Li Y,Hu Y,An LY,Yang L,Zhang J,Chen YE,Xu J

更新日期：2015-12-01 00:00:00

abstract：:Male germline stem cells (mGSCs) are stem cells present in male testis responsible for spermatogenesis during their whole life. Studies have shown that mGSCs can be derived in vitro and resemble embryonic stem cells (ESCs) properties both in the mouse and humans. However, little is know about these cells in domestic a...

journal_title：Cellular reprogramming

authors： Hua J,Zhu H,Pan S,Liu C,Sun J,Ma X,Dong W,Liu W,Li W

更新日期：2011-04-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) remain a promising approach to target diseases with a loss of functional parenchyma. This technology comes with a number of concerns for clinical applications, including teratogenic potential and genomic instability. Here we focused on evaluating the safety of cross-species Senda...

journal_title：Cellular reprogramming

authors： Tarnawski L,Eugster E,DeCamp L,Jovinge S

更新日期：2019-04-01 00:00:00

abstract：:Demecolcine-assisted/induced enucleation has been used in nuclear transfer cloning procedures for many species, yet its mechanism of action remains unclear. Primarily because oocytoplasm protrusion induced by demecolcine is inhibited by the presence of cytochalasin, its use has had limited application. In this experim...

journal_title：Cellular reprogramming

authors： Meng Q,Wu X,Bunch TD,White K,Sessions BR,Davies CJ,Rickords L,Li GP

更新日期：2011-10-01 00:00:00

abstract：:Somatic cell nuclear transfer (SCNT), commonly referred to as cloning, results in the generation of offspring that, except for mitochondrial DNA, are genetically identical to the nuclear donor. We previously used a genetically modified bovine cell line as the donor for SCNT and obtained a calf, named Daisy, that was b...

journal_title：Cellular reprogramming

authors： Wagner S,Cullum A,Wells DN,Laible G

更新日期：2017-12-01 00:00:00

abstract：:Previous studies have shown that the time interval between fusion and activation (FA interval) play an important role in nuclear remodeling and in vitro development of somatic cell nuclear transfer (SCNT) embryos. However, the effects of FA interval on the epigenetic reprogramming and in vivo developmental competence ...

journal_title：Cellular reprogramming

authors： Liu J,Wang Y,Su J,Wang L,Li R,Li Q,Wu Y,Hua S,Quan F,Guo Z,Zhang Y

更新日期：2013-04-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) play an important role in cell replacement therapy. Several studies have shown that keratinocytes are promising reprogrammed cells. We easily and efficiently enriched epidermal stem cells by attaching them for a limited time in culture dishes. Individual epidermal cells enriched ...

journal_title：Cellular reprogramming

authors： Yang YH,Zhang RZ,Cheng S,Xu B,Tian T,Shi HX,Xiao L,Chen RH

更新日期：2018-12-01 00:00:00

abstract：:The purpose of this study was to investigate the effects of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on transgene expression and development of porcine transgenic cloned embryos, specifically focusing on effects derived from TSA-treated donor cells or TSA-treated reconstructed embryos. The results...

journal_title：Cellular reprogramming

authors： Luo B,Ju S,Muneri CW,Rui R

更新日期：2015-02-01 00:00:00

abstract：:Methyl-CpG-binding domain proteins (MBPs) connect DNA methylation and histone modification, which are the key changes of somatic cell reprogramming. Methyl-CpG-binding protein 2 (MeCP2) was the first discovered MBP that has been extensively studied in the neurodevelopmental disorder Rett syndrome. However, a role for ...

journal_title：Cellular reprogramming

authors： Wang ZD,Duan L,Zhang ZH,Song SH,Bai GY,Zhang N,Shen XH,Shen JL,Lei L

更新日期：2016-04-01 00:00:00

abstract：:Although numerous mammalian species have been successfully cloned by somatic cell nuclear transfer (SCNT), little is known about gene expression of cloned pigs by SCNT. In the present study, expression profiles of 1-month-old cloned pigs generated from fetal fibroblasts (n = 5) were compared to those of age-matched co...

journal_title：Cellular reprogramming

authors： Park J,Lai L,Samuel M,Wax D,Bruno RS,French R,Prather RS,Yang X,Tian XC

更新日期：2011-06-01 00:00:00

abstract：:Induced pluripotent stem cell (iPSC) technology has become an important tool for disease modeling. Insufficient data on the variability among iPSC lines derived from a single somatic parental cell line have in practice led to generation and analysis of several, usually three, iPSC sister lines from each parental cell ...

journal_title：Cellular reprogramming

authors： Schuster J,Halvardson J,Pilar Lorenzo L,Ameur A,Sobol M,Raykova D,Annerén G,Feuk L,Dahl N

更新日期：2015-10-01 00:00:00

abstract：:The goals of the current study were to (1) improve culture conditions and (2) chemical passaging of bovine embryonic stem cell-like (bESC-like) cells. Specifically, the effects of human leukemia inhibitory factor (hLIF), two types of feeders, mouse embryonic fibroblast (MEF) and bovine embryonic fibroblast (BEF), as w...

journal_title：Cellular reprogramming

authors： Gong G,Roach ML,Jiang L,Yang X,Tian XC

更新日期：2010-04-01 00:00:00

abstract：:In contrast to adult stem cells, induced pluripotent stem cells (iPSCs) can be grown robustly in vitro and differentiated into virtually any tissue, thus providing an attractive alternative for biomedical applications. Although iPSC technology is already being used in human biomedicine, its potential in animal product...

journal_title：Cellular reprogramming

authors： Cravero D,Martignani E,Miretti S,Accornero P,Pauciullo A,Sharma R,Donadeu FX,Baratta M

更新日期：2015-06-01 00:00:00

abstract：:Adipose tissue-derived stromal cells (ADSCs) have been identified as a powerful stem cell source for cellular transplantation therapy. The dog is increasingly used as a model of human neurological disease; however, few studies have reported induction of canine ADSCs to neural lineages. We characterized canine ADSCs an...

journal_title：Cellular reprogramming

authors： Lim JH,Boozer L,Mariani CL,Piedrahita JA,Olby NJ

更新日期：2010-08-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) are promising candidates for the study of disease models as well as for tissue engineering purposes. Part of a strategy to develop safe reprogramming technique is reducing the number of exogenous reprogramming factors. Some cells types are more prone to reprogramming than others....

journal_title：Cellular reprogramming

authors： Ma MS,Czepiel M,Krause T,Schäfer KH,Boddeke E,Copray S

更新日期：2014-10-01 00:00:00

abstract：:The less differentiated the donor cells are used in nuclear transfer (NT), the more easily are they reprogrammed by the recipient cytoplasm. In this context, mesenchymal stem cells (MSCs) appear as an alternative to donor nuclei for NT. The amniotic fluid and adipose tissue are sources of MSCs that have not been teste...

journal_title：Cellular reprogramming

authors： da Silva CG,Martins CF,Cardoso TC,da Cunha ER,Bessler HC,Martins GH,Pivato I,Báo SN

更新日期：2016-04-01 00:00:00

abstract：:Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined ...

journal_title：Cellular reprogramming

authors： Peng X,Liu T,Yang B,Shi C,Sun Y,Jiang L,Jin H,Li L,Zhu H,Wu M,Qian Q

更新日期：2013-06-01 00:00:00

abstract：:Successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos depends on compatibilities between ooplasmic and nuclear components. However, the mechanisms by which the compatibilities are regulated are still unknown. In this study, using mouse Oct4-green fluorescent protein (GFP) cells as donors...

journal_title：Cellular reprogramming

authors： Huang X,Song L,Zhan Z,Gu H,Feng H,Li Y

更新日期：2017-12-01 00:00:00

abstract：:Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of ...

journal_title：Cellular reprogramming

authors： Sekhavati MH,Shadanloo F,Hosseini MS,Tahmoorespur M,Nasiri MR,Hajian M,Nasr-Esfahani MH

更新日期：2012-08-01 00:00:00

abstract：:The differentiation of multipotent stem cells toward a pancreatic lineage provides us with an alternative cell-based therapeutic approach to type 1 diabetes and enables us to study pancreas development. The current study aims to study the effect of growth factors such as activin A or nicotinamide, alone and in combina...

journal_title：Cellular reprogramming

authors： Balaji S,Zhou Y,Opara EC,Soker S

更新日期：2017-08-01 00:00:00

abstract：:Factor-induced reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) as a powerful tool for regenerative medicine has gained wide attention in recent years. However, there are certain concerns regarding the efficiency of this reprogramming. Partially reprogrammed iPSCs (piPSCs) are stable cell lin...

journal_title：Cellular reprogramming

authors： Zhang L,Wang Y,Zhang Y,Wang L,Huang H

更新日期：2020-04-01 00:00:00

abstract：:As man's best friend, dogs have an important position in human society. Ten years ago, we reported the first cloned dog, and his birth has raised various scientific issues, such as those related to health, reproduction, and life span. He has developed without any unique health issues. In this article, we summarize and...

journal_title：Cellular reprogramming

authors： Jang G,Lee B

更新日期：2015-10-01 00:00:00

abstract：:Genetically modified pigs are commonly created via somatic cell nuclear transfer (SCNT). Treatment of reconstructed embryos with histone deacetylase inhibitors (HDACi) immediately after activation improves cloning efficiency. The objective of this experiment was to evaluate the transcriptome of SCNT embryos treated wi...

journal_title：Cellular reprogramming

authors： Whitworth KM,Mao J,Lee K,Spollen WG,Samuel MS,Walters EM,Spate LD,Prather RS

更新日期：2015-08-01 00:00:00

abstract：:Chickens are vitally important in numerous countries as a primary food source and a major component of economic development. Efforts have been made to produce transgenic birds through pluripotent stem cell [primordial germ cells and embryonic stem cells (ESCs)] approaches to create animals with improved traits, such a...

journal_title：Cellular reprogramming

authors： Yu P,Lu Y,Jordan BJ,Liu Y,Yang JY,Hutcheson JM,Ethridge CL,Mumaw JL,Kinder HA,Beckstead RB,Stice SL,West FD

更新日期：2014-10-01 00:00:00

abstract：:The generation of defined somatic cell types from pluripotent stem cells represents a promising system for many applications for regenerative therapy or developmental studies. Certain key developmental genes have been shown to be able to influence the fate determination of differentiating stem cells suggesting an alte...

journal_title：Cellular reprogramming

authors： Thoma EC,Maurus K,Wagner TU,Schartl M

更新日期：2012-04-01 00:00:00

abstract：:In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as recepto...

journal_title：Cellular reprogramming

authors： Naaman H,Rabinski T,Yizhak A,Mizrahi S,Avni YS,Taube R,Rager B,Weinstein Y,Rall G,Gopas J,Ofir R

更新日期：2018-02-01 00:00:00

abstract：:The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine (5-aza-dC), an inhibitor of DNA methyltransferases, are widely used for alteri...

journal_title：Cellular reprogramming

authors： Saini M,Selokar NL,Agrawal H,Singla SK,Chauhan MS,Manik RS,Palta P

更新日期：2017-06-01 00:00:00