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Angiostrongylus cantonensis: identification and characterization of microRNAs in male and female adults.

Abstract：

:Angiostrongylus cantonensis causes eosinophilic meningitis and eosinophilic pleocytosis in humans and is of significant socio-economic importance globally. microRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in gene expression regulation, cellular function and defense, homeostasis and pathogenesis. They have been identified in a diverse range of organisms. The objective of this study was to determine and characterize miRNAs of female and male adults of A. cantonensis by Solexa deep sequencing. A total of 8,861,260 and 10,957,957 high quality reads with 20 and 23 conserved miRNAs were obtained in females and males, respectively. No new miRNA sequence was found. Nucleotide bias analysis showed that uracil was the prominent nucleotide, particularly at positions of 1, 10, 14, 17 and 22, approximately at the beginning, middle and the end of the conserved miRNAs. To our knowledge, this is the first report of miRNA profiles in A. cantonensis, which may represent a new platform for studying regulation of genes and their networks in A. cantonensis.

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

Chen MX,Ai L,Xu MJ,Zhang RL,Chen SH,Zhang YN,Guo J,Cai YC,Tian LG,Zhang LL,Zhu XQ,Chen JX

doi

10.1016/j.exppara.2011.02.019

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

116-20

issue

2

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(11)00081-6

journal_volume

128

pub_type

杂志文章

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